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Process for producing arginine by microbial fermentation

A microbial fermentation and arginine technology, applied in the direction of microorganisms, microorganism-based methods, fermentation, etc., can solve the problems of product quality insecurity, labor and time-consuming, low yield, etc., to reduce product damage and improve filtrate quality , The effect of improving product yield

Inactive Publication Date: 2014-04-02
滨州市生物技术研究院有限责任公司
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AI Technical Summary

Benefits of technology

This patented technical effect described by this patents relates to replacing conventional methods with new techniques like filter or membranes rather than just removing impurities during production processes. By utilizing these technologies, better purification results are achieved without increasing costs associated therewith. Additionally, it provides an improved efficiency compared to existing systems due to reduced space requirements needed per unit volume processed. Overall, this innovative approach helps improve the overall performance and output of biological manufacturing processes such as cell culture growth media.

Problems solved by technology

The technical problem addressed in this patents relates to improving the efficiency or producing high purity arginines from natural sources like plants that have potential uses in medications, dietary supplements, cosmetic compositions, feedstock materials, etc., while reducing their impact upon human bodies through negative effects associated with excessive consumption.

Method used

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Embodiment １

[0030] The preparation of fermented liquid comprises the following steps:

[0031] (1) Slant culture: insert the MQA121 strain into the sterilized slant medium (formula glucose 2.0-2.5%, (NH 4 ) 2 SO 4 0.5-0.8%, KH 2 PO 4 0.1-0.3%, K 2 HPO 4 ·3H 2 O0.3-0.6%, MgSO 4 ·7H 2 O0.05-0.1%, FeSO 4 ·7H 2 O 0.002-0.01%, MnSO 4 ·H 2 O0.002-0.008%, agar powder 2.0%, pH7.0~7.4, cultivated at 28°C, and obtained slant bacteria for 28 hours;

[0032] (2) Shake flask strain: put the cultured slant strain into the shake flask sterilized seed medium and cultivate at 28°C for 22 hours to obtain the shake flask seed liquid;

[0033] (3) First-level seed culture: put the cultivated shake flask seed solution into the sterilized first-level seed medium, and cultivate it at 28°C for 14 hours to obtain the first-level seed solution;

[0034] (4) Secondary seed culture: put the cultivated primary seed liquid into the sterilized secondary seed medium and cultivate it at 28°C for 4 hours to ...

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Abstract

The invention discloses a process for producing arginine by microbial fermentation. Brevibacterium flavum MQA121 is adopted. The method comprises the following steps: preparing fermentation liquid, namely performing slant culture, performing shake-flask culture, performing primary seed culture, performing secondary seed culture, and continuing to ferment; extracting and refining a fermented product, namely preprocessing the fermentation liquid, performing secondary crystallization, performing color and impurity removal, performing membrane concentration and separation, drying, and packaging to obtain arginine as a finished product. The method has the benefits that organic and inorganic matters and bacteria in subsequent extracting and refining liquid of arginine can be remarkably reduced and the arginine yield can be increased; in addition, the product quality is improved and the production cost is reduced.

Description

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Claims

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Application Information

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Owner 滨州市生物技术研究院有限责任公司
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