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Brevibacterium strain and method for in-situ remediation of heavy metal polluted farmland using same

A Brevibacterium and farmland technology, applied in the field of applied microbiology, can solve problems such as fertility decline, pollution, and soil compaction

Active Publication Date: 2018-07-03
有研资源环境技术研究院(北京)有限公司
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Problems solved by technology

[0002] Heavy metal pollution in my country's farmland is serious, and the existing treatment methods are mainly chemical passivation, and a considerable part of chemical passivation agents (such as lime, sodium sulfide, etc.) Indigenous microbial communities in the soil, causing problems such as soil compaction and fertility decline
On the other hand, 74% of the cultivated land in my country is deficient in phosphorus. Because the applied phosphorus fertilizer is easily solidified, 95% or more of the phosphorus exists in an ineffective form that is difficult to use. The excessive addition of phosphorus fertilizer in farmland further causes soil pollution compaction

Method used

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  • Brevibacterium strain and method for in-situ remediation of heavy metal polluted farmland using same
  • Brevibacterium strain and method for in-situ remediation of heavy metal polluted farmland using same

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Embodiment 1

[0032] The Brevibacterium sp. provided by the present invention has been preserved, and the classification of the bacterium is named Brevibacterium sp. GRINM L2. The depository unit: General Microbiology Center of China Microbiological Culture Collection Management Committee, and the address is: Beichen West Road, Chaoyang District, Beijing No. 1 Courtyard No. 3, Institute of Microbiology, Chinese Academy of Sciences, date of deposit: September 29, 2016, deposit number: CGMCC No.13064.

[0033] The culture method of Brevibacterium described in the present invention is as follows:

[0034] First prepare the medium (liquid medium) for enriching and cultivating Brevibacteria: 10g / L glucose, 5g / L calcium phosphate, 0.3g / L magnesium sulfate heptahydrate, 0.5g / L ammonium sulfate, 0.3g / L sodium chloride , 0.3g / L potassium chloride, 0.002g / L manganese sulfate, 0.03g / L ferrous sulfate heptahydrate, 1g / L yeast extract, 1L water, pH7.3.

[0035] Solid medium: 10g / L glucose, 5g / L calcium...

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Abstract

The invention provides a brevibacterium strain and a method for in-situ remediation of heavy metal polluted farmland by using the same. The class name of the brevibacterium is named: Brevibacterium spGRINM L2; and the brevibacterium is collected by the China General Microbiological Culture Collection Center, Institute of Microbiology, Chinese Academy of Sciences, 3#, No.1 Courtyard, West BeichenRoad, Chaoyang District, Beijing, on September 29, 2016 and with a collection number: CGMCC No.13064. In the presence of high-concentration heavy metal ions, the bacteria can efficiently convert insoluble phosphate into soluble phosphate and can be applied to the remediation of heavy metal polluted farmland.

Description

technical field [0001] The invention belongs to the field of applied microbiology, and relates to a strain of brevibacterium, which can survive in the presence of high-concentration heavy metal ions, convert insoluble phosphate into soluble phosphate, and the brevibacterium is used for in-situ repair of farmland heavy metal pollution method. Background technique [0002] Heavy metal pollution in my country's farmland is serious, and the existing treatment methods are mainly chemical passivation, and a considerable part of the chemical passivation agents (such as lime, sodium sulfide, etc.) The indigenous microbial community in the soil causes problems such as soil compaction and fertility decline. On the other hand, 74% of the cultivated land in my country is deficient in phosphorus. Because the applied phosphorus fertilizer is easily solidified, 95% or more of the phosphorus exists in an ineffective form that is difficult to use. of hardening. Therefore, if the phosphorus-...

Claims

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Application Information

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IPC IPC(8): C12N1/20C09K17/14B09C1/10C09K101/00C12R1/13
CPCB09C1/10C09K17/14C09K2101/00C12N1/20C12N1/205C12R2001/13
Inventor 刘兴宇张明江谷启源温建康
Owner 有研资源环境技术研究院(北京)有限公司
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