Ice clara health-care tea and preparation method thereof

Abstract

The invention discloses ice clara health-care tea and a preparation method thereof, and belongs to the technical field of health-care tea. The ice clara health-care tea comprises an ice clara extract, an aloe extract, an American ginseng extract, composite ferment powder and composite enzymolysis powder, the composite ferment powder is obtained by fermenting a schisandra chinensis extract, an acanthopanax senticosus extract and a folium cortex eucommiae extract through a composite fermentation inoculant and drying; the compound fermentation inoculant comprises clostridium butyricum, bacillus coagulans and aspergillus oryzae; the compound enzymolysis powder is obtained by mixing radix polygonati officinalis, burdock and echinacea purpurea and then performing enzymolysis with compound enzyme; the compound enzyme comprises pectinase, acid protease, glucose oxidase, malate dehydrogenase and thermolysin. According to the ice clara health-care tea disclosed by the invention, the solubility and bioavailability of the product are improved, and the stability and functionality of the ice clara health-care tea are also enhanced, so that the product has a remarkable advantage in the aspect of improving the immunity, and the product can better meet the requirements of consumers on health care through the elaborately designed process steps.

Description

Technical Field

[0001] The invention belongs to the technical field of health-care tea, and particularly relates to snow cherry fruit health-care tea and a preparation method thereof. Background Art

[0002] Snow cherry is a unique plant, belonging to the annual Amaranthaceae family. It is not only ornamental, but also has high nutritional and medicinal value. The height of the snow cherry plant is about 1.5 meters. The stems are bright red when the seedlings are young, and gradually turn light green as the plant grows. The leaves of the snow cherry can be eaten as a vegetable and have a unique taste. Snow cherry prefers a warm, humid and sunny environment. It can grow in a variety of soils, but it is best in fertile, well-drained soil.

[0003] The nutritional components of snow cherry seeds include protein, fat, carbohydrates, carotene, B vitamins, minerals and plant hormones. In addition to the main functions mentioned above, snow cherry seeds can also aid digestion, strengthen the stomach, open the chest, soothe the liver, promote salivation, quench thirst, replenish blood, strengthen sperm, nourish the heart, and calm the nerves. Therefore, snow cherry seeds have more comprehensive effects.

[0004] At present, the research on snow cherry seeds mainly focuses on planting technology, analysis of pharmacologically active ingredients, and extraction of active ingredients. However, there are relatively few studies on processing snow cherry seeds into instant tea or beverages. Developing such products can not only increase the value chain of snow cherry seeds, but also allow more people to enjoy its unique health benefits. To achieve this goal, key issues that need to be addressed include how to keep the active ingredients of snow cherry seeds from being destroyed, how to improve the aroma and solubility of the product, and how to maximize the health benefits of snow cherry seeds. Summary of the invention

[0005] The purpose of the present invention is to provide a snow cherry fruit health tea and a preparation method thereof, so as to solve the problems that the effective ingredients of the snow cherry fruit health tea are easily destroyed, the solubility is poor, and the health care effect is not good. The snow cherry fruit health tea of ​​the present invention not only improves the solubility and bioavailability of the product, but also enhances its stability and functionality, so that the product has significant advantages in improving immunity. Through these carefully designed process steps, the product can better meet consumers' needs for health care.

[0006] To achieve the above object, the first aspect of the present invention provides a snow cherry health tea, which comprises the following raw materials in parts by weight:

[0007] 40-60 parts of Snow Cherry Extract

[0008] 5-10 parts of aloe vera extract

[0009] 1-5 portions of American ginseng extract

[0010] 10-15 parts of compound baking powder

[0011] 15-20 portions of compound enzymatic hydrolysis powder;

[0012] The composite fermentation powder is obtained by fermenting and drying schisandra chinensis extract, acanthopanax senticosus extract and eucommia leaf extract through a composite fermentation agent; the mass ratio of schisandra chinensis extract, acanthopanax senticosus extract and eucommia leaf extract is (3-5):(7-10):1; the composite fermentation agent includes Clostridium butyricum, Bacillus coagulans and Aspergillus oryzae in a mass ratio of 1:(2-4):(6-8);

[0013] The composite enzymatic hydrolysis powder comprises enzymatic hydrolysis powder of polygonatum odoratum, enzymatic hydrolysis powder of burdock and enzymatic hydrolysis powder of echinacea. The enzymatic hydrolysis powder of polygonatum odoratum, enzymatic hydrolysis powder of burdock and enzymatic hydrolysis powder of echinacea are obtained by mixing polygonatum odoratum, burdock and echinacea and then enzymolyzing them with composite enzyme. The added mass of composite enzyme is 5-10% of the total mass of polygonatum odoratum, burdock and echinacea, and the mass ratio of polygonatum odoratum, burdock and echinacea is (5-8):1:(2-4); the composite enzyme comprises pectinase, acid protease, glucose oxidase, malate dehydrogenase and thermophilic protease according to the mass ratio of (8-10):(2-4):(3-5):1:1.

[0014] Clostridium butyricum was purchased from Beijing Kezhan Biotechnology Co., Ltd., model number BNCC185375.

[0015] Bacillus coagulans was purchased from Beijing Kezhan Biotechnology Co., Ltd., model number BNCC132591.

[0016] Aspergillus oryzae was purchased from Beijing Kezhan Biotechnology Co., Ltd., model number BNCC144211.

[0017] Pectinase was purchased from Shanghai Yuanye Biotechnology Co., Ltd., model number S10007.

[0018] Acidic protease was purchased from Shanghai Yuanye Biotechnology Co., Ltd., model number S10012.

[0019] Glucose oxidase was purchased from Shanghai Yuanye Biotechnology Co., Ltd., model number S10020.

[0020] Malate dehydrogenase was purchased from Shanghai Yuanye Biotechnology Co., Ltd., model number S10168.

[0021] Thermolysin was purchased from Shanghai Yuanye Biotechnology Co., Ltd., model number S10236.

[0022] Health tea refers to a drink that has specific health-effect ingredients added to ordinary tea leaves, and is usually made from plant leaves, flowers, roots, fruits, etc. There are many types of health teas, and their effects and tastes are also different. Although the current health tea has certain effects, the real effect is not satisfactory, and it also has the disadvantages of low solubility in water, unstable properties, and easy turbidity after being placed for a period of time. The present invention uses snow cherry fruit extract as the main ingredient, and uses aloe extract American ginseng extract, composite fermentation powder and composite enzymatic hydrolysis powder to improve the effect of health tea products in enhancing immunity. The snow cherry fruit in the present invention contains a variety of immunomodulatory factors, which can enhance the body's immunity. The snow cherry fruit extract prepared by the extraction method is rich in these active ingredients and is the basis for enhancing immunity. Aloe extract has good anti-inflammatory and antioxidant properties, which can help reduce inflammatory reactions, reduce free radical damage, and then support the normal functioning of the immune system. American ginseng extract can help relieve stress and fatigue, enhance physical strength, and thus indirectly support the function of the immune system. The Chinese medicinal materials such as Schisandra chinensis, Acanthopanax senticosus and Eucommia ulmoides in the composite fermentation powder can produce more biologically active metabolites through fermentation, which help to enhance immune function. The active ingredients in plants such as Polygonatum odoratum, Burdock and Echinacea purpurea in the composite enzymatic hydrolysis powder are released through enzymatic hydrolysis, and these ingredients have antioxidant and immunomodulatory effects. In the present invention, by mixing these ingredients in a certain proportion, a synergistic system can be formed, in which the snow cherry fruit extract is used as the core ingredient, and the other ingredients play a role in assisting and enhancing the efficacy of the snow cherry fruit.

[0023] The present invention continues to study and find that although the synergistic effect of various substances can improve the effect of health tea products in enhancing immunity, the solubility of health tea dissolved in water is affected, precipitation is easy to occur, and turbidity is easy to occur after being placed for a period of time, and the stability is poor. In view of this problem, the present invention continues to study and find that when preparing the composite fermentation powder, the mass ratio of the three extracts of Schisandra chinensis extract, Acanthopanax senticosus extract and Eucommia ulmoides leaf extract is limited, and the mass ratio of the three fermentation bacteria of Clostridium butyricum, Bacillus coagulans and Aspergillus oryzae is limited. At the same time, in the preparation process of the composite enzymatic hydrolysis powder, the mass ratio of Polygonatum odoratum, Burdock and Echinacea is limited, and the mass ratio of pectinase, acid protease, glucose oxidase, malate dehydrogenase and thermophilic protease is limited, which can improve the solubility and stability of the health tea product. Analysis of the reasons: During the fermentation process of the three extracts, the action of microorganisms can decompose macromolecules (such as polysaccharides, proteins, etc.) into smaller molecules, which are more soluble in water, thereby improving solubility. The metabolites produced during the fermentation process will change the surface properties of the substances, making them more easily dispersed in water and improving solubility. Fermentation can make the ingredients more uniform and consistent, reducing the precipitation or turbidity caused by uneven ingredients. The enzymes produced during the fermentation process can help decompose those difficult-to-dissolve ingredients, thereby reducing the turbidity or precipitation that may occur after dissolution. The various enzymes in the composite enzymatic hydrolysis powder can efficiently decompose cellulose and other macromolecules in the plant cell wall, converting them into smaller molecules, thereby improving solubility. The products after enzymatic hydrolysis usually have better water solubility, which means that they can be better dispersed in water, reducing the problems that may occur during the dissolution process. During the enzymatic hydrolysis process, macromolecules that are difficult to dissolve in water are decomposed into smaller molecules, which are more soluble in water, thereby reducing the risk of precipitation and turbidity. Enzymatic hydrolysis can make the active ingredients more stable and not easy to aggregate in water to form precipitation or turbidity. The present invention can not only improve the solubility of the effective ingredients of health tea by using composite fermentation powder and composite enzymatic hydrolysis powder, but also reduce the turbidity that may occur during the dissolution process, thereby improving the overall quality of the product and the experience of consumers.

[0024] Preferably, the preparation method of the snow cherry fruit extract is:

[0025] (1) Pretreatment: fresh leaves are picked from snow cherry plants as raw materials, mixed with an 8-10% vitamin C aqueous solution to obtain a mixture, the mixture is placed at -10-5°C for 60-90 minutes, thawed at room temperature, a 3-5% sodium bicarbonate aqueous solution is added, heated in a water bath at 50-60°C for 5-10 hours, and filtered to obtain a pretreated solution;

[0026] (2) Resin treatment: Add the pre-treated solution to a loaded macroporous adsorption resin column, filter through the resin column, elute with ethanol-water solution, and collect and combine all the eluates;

[0027] (3) Concentration and drying: The eluate is concentrated under reduced pressure and dried to obtain the snow cherry fruit extract.

[0028] Preferably, the mass ratio of the leaf raw material to the vitamin C aqueous solution is 1:8-10.

[0029] Preferably, the volume ratio of the mixture to the sodium bicarbonate aqueous solution is 1:0.01-0.05.

[0030] Preferably, the macroporous adsorption resin column is packed with ADS-800 grade adsorption resin.

[0031] ADS-800 grade adsorption resin was purchased from Kehaisi (Beijing) Technology Co., Ltd.

[0032] The present invention uses a specific extraction method to extract the effective ingredients in the snow cherry fruit, which not only improves the efficacy, solubility and stability of the snow cherry fruit extract, but also improves the overall quality of the health tea, making the product more competitive in the market. Analysis of the reasons: In the pretreatment stage, a vitamin C aqueous solution is used to mix with the snow cherry fruit, and the freezing treatment is carried out at a low temperature, which can effectively protect the active ingredients in the snow cherry fruit from being destroyed. Vitamin C, as an antioxidant, can prevent the effective ingredients from being oxidized during the treatment process, thereby ensuring its biological activity. Sodium bicarbonate water bath treatment can change the physicochemical properties of the effective ingredients of the snow cherry fruit to a certain extent, making it easier to be absorbed and utilized by the human body, thereby enhancing the efficacy of the health tea. Through sodium bicarbonate water bath treatment, the solubility of the snow cherry fruit extract can be changed, so that it can be dissolved faster and more completely in water, which is very beneficial for improving the solubility of the health tea. The improvement of solubility also means that the health tea will be more uniform when brewing, without obvious granularity, thereby improving the taste. The present invention uses a macroporous adsorption resin for filtration, which can remove impurities in the extract and improve the purity of the effective ingredients, which is very critical to improving the stability of the product. The present invention uses an ethanol aqueous solution of a specific concentration for elution, which can ensure the stable extraction of effective components and avoid unstable factors that may be caused by excessive extraction.

[0033] Preferably, the specific elution process of the resin column is: firstly eluting with 1 to 3 times the volume of the column with a volume fraction of 5 to 8% ethanol aqueous solution, and then eluting with 5 to 8 times the volume of the column with a volume fraction of 50 to 65% ethanol aqueous solution.

[0034] Preferably, the preparation method of the composite baking powder is:

[0035] (1) mixing the Schisandra chinensis extract, the Acanthopanax senticosus extract and the Eucommia ulmoides leaf extract in proportion to obtain a mixture;

[0036] (2) inoculating 6 to 8 wt% of a composite fermentation agent into the mixture, continuously introducing air, fermenting at 25 to 35° C. and 50 to 75% humidity for 5 to 10 days, and filtering to obtain a fermentation liquid;

[0037] (3) Concentrating and drying the fermentation liquid to obtain composite fermentation powder.

[0038] Preferably, the preparation method of the composite enzymatic hydrolysis powder is:

[0039] (1) Washing polygonatum odoratum, burdock and echinacea, chopping them, air-drying them, drying them at 55-65° C. for 1-3 hours, baking them at 160-180° C. for 20-40 minutes, and ultrafine grinding them to obtain raw material powder;

[0040] (2) adding water to the raw material powder in an amount of 8 to 12 times the mass of the raw material powder, mixing evenly, adjusting the pH value to 6 to 7, adding a composite enzyme at 30 to 45° C., and performing enzymolysis for 5 to 8 hours to obtain an enzymolysis solution;

[0041] (3) The enzymatic hydrolysate is concentrated, dried, and ultrafinely ground to obtain a composite enzymatic hydrolysate powder.

[0042] The second aspect of the present invention provides a method for preparing snow cherry health tea, comprising the following steps:

[0043] a) grinding and mixing the snow cherry fruit extract, aloe extract, American ginseng extract, composite fermentation powder and composite enzymatic hydrolysis powder in proportion to obtain a material;

[0044] b) adding water to the material and stirring at 100-120° C. for 2-5 hours, wherein the amount of water added is 5-8 times of the material, cooling to room temperature, standing for 5-10 hours, and filtering to obtain a mixed solution;

[0045] c) the mixed solution is concentrated and dried to obtain the snow cherry health tea.

[0046] Therefore, the present invention adopts the above-mentioned snow cherry health tea and its preparation method, which has the following beneficial effects:

[0047] 1. The present invention uses a vitamin C aqueous solution for mixing and freezing treatment at low temperature, which can effectively protect the active ingredients in the snow cherry fruit from being destroyed. Vitamin C is a powerful antioxidant that can prevent the plant extract from being oxidized during the treatment process, thereby protecting the integrity of the active ingredients. The subsequent sodium bicarbonate water bath treatment further improves the solubility of these ingredients, making them more easily absorbed and utilized by the human body, and improving the solubility and bioavailability of the active ingredients of the snow cherry fruit.

[0048] 2. The present invention uses a macroporous adsorption resin to filter and elute the pretreatment liquid, which can remove impurities and concentrate effective ingredients. This method can not only improve the purity of the effective ingredients, but also enhance the stability of the product, which helps to extend the shelf life of the product. Using a specific concentration of ethanol aqueous solution for elution can not only improve the purity of the effective ingredients, but also further stabilize these ingredients by changing the solvent environment, ensuring the quality of the product under long-term storage. The present invention helps to enhance the stability of the product and extend the shelf life.

[0049] 3. The present invention can produce a variety of beneficial metabolites, such as polysaccharides, organic acids, etc., through the fermentation of Chinese medicinal materials such as Schisandra chinensis, Acanthopanax senticosus and Eucommia ulmoides leaves. These ingredients help to enhance human immunity. The metabolites produced during the fermentation process often have higher biological activity, can better stimulate the response of the immune system, and help improve the body's resistance.

[0050] 4. The complex enzyme used in the enzymatic hydrolysis process of the present invention can more effectively release the active ingredients in Polygonatum odoratum, Burdock and Echinacea purpurea, which have antioxidant and immunomodulatory effects. The enzymatic hydrolysis technology can efficiently decompose cell walls and release more bioactive substances, thereby enhancing the function of the immune system.

[0051] 5. The formula of the present invention contains a variety of natural plant extracts such as snow cherry, aloe vera, American ginseng, etc. These ingredients can work together to exert a synergistic effect, thereby enhancing the overall health-promoting effect. For example, American ginseng helps relieve fatigue, while aloe vera has good anti-inflammatory and repair effects. These ingredients work together to improve physical condition. BRIEF DESCRIPTION OF THE DRAWINGS

[0052] Figure 1 This is a comparison of the fluorescence intensities of the blank control group, model control group, positive control group and the sample treatment group of Example 1. In the figure, * means P<0.05, ** means P<0.01, *** means P<0.001, and the more *, the smaller the P value, the more significant the difference;

[0053] Figure 2 It is the phenotype diagram of the blank control group, model control group, positive control group and the sample treatment group of Example 1 (magnification: 40 times). DETAILED DESCRIPTION

[0054] The present invention will be further described below. It should be noted that this embodiment is based on the technical solution and provides a detailed implementation method and a specific operation process, but the present invention is not limited to this embodiment.

[0055] Example 1

[0056] This embodiment provides a snow cherry health tea, which includes the following raw materials in parts by weight:

[0057] Snow cherry fruit extract 50 parts

[0058] 8 parts of aloe vera extract

[0059] 3 parts American ginseng extract

[0060] 12 servings of compound baking powder

[0061] 18 servings of compound enzymatic powder.

[0062] The preparation method of the snow cherry fruit extract is as follows:

[0063] (1) Pretreatment: fresh leaves were picked from snow cherry plants as raw materials, mixed with a 9% vitamin C aqueous solution to obtain a mixture, the mass ratio of the leaf raw material to the vitamin C aqueous solution was 1:8, the mixture was frozen at -8°C for 80 minutes, thawed at room temperature, a 4% sodium bicarbonate aqueous solution was added, the volume ratio of the mixture to the sodium bicarbonate aqueous solution was 1:0.03, heated in a water bath at 55°C for 8 hours, and filtered to obtain a pretreated solution;

[0064] (2) Resin treatment: The pretreatment liquid was added to a loaded macroporous adsorption resin column, which was loaded with ADS-800 grade adsorption resin. After filtering through the resin column, it was first eluted with a 6% ethanol aqueous solution with a volume fraction of 2 times the column volume, and then eluted with a 60% ethanol aqueous solution with a volume fraction of 6 times the column volume. All the eluates were collected and combined; ADS-800 grade adsorption resin was purchased from Kehaisi (Beijing) Technology Co., Ltd.

[0065] (3) Concentration and drying: The eluate is concentrated under reduced pressure and dried to obtain the snow cherry fruit extract.

[0066] The preparation method of composite baking powder is:

[0067] (1) mixing the schisandra extract, the acanthopanax extract and the eucommia leaf extract in a proportion such that the mass ratio of the schisandra extract, the acanthopanax extract and the eucommia leaf extract is 4:8:1 to obtain a mixture;

[0068] (2) inoculating the mixture with 7 wt% of a composite fermentation agent, wherein the composite fermentation agent includes Clostridium butyricum, Bacillus coagulans and Aspergillus oryzae in a mass ratio of 1:3:7, continuously introducing air, fermenting at 30° C. and 65% humidity for 8 days, and filtering to obtain a fermentation liquid;

[0069] Clostridium butyricum was purchased from Beijing Kezhan Biotechnology Co., Ltd., model number BNCC185375.

[0070] Bacillus coagulans was purchased from Beijing Kezhan Biotechnology Co., Ltd., model number BNCC132591.

[0071] Aspergillus oryzae was purchased from Beijing Kezhan Biotechnology Co., Ltd., model number BNCC144211.

[0072] (3) Concentrating and drying the fermentation liquid to obtain composite fermentation powder.

[0073] The preparation method of the composite enzymatic hydrolysis powder is as follows:

[0074] (1) Washing polygonatum odoratum, burdock and echinacea, chopping them, air-drying them, drying them at 60°C for 2 hours, baking them at 165°C for 30 minutes, and ultrafine grinding them to obtain raw material powder; the mass ratio of polygonatum odoratum, burdock and echinacea is 6:1:3;

[0075] (2) adding water to the raw material powder in an amount of 10 times the weight of the raw material powder, mixing evenly, adjusting the pH value to 7, adding a composite enzyme at 40° C., wherein the added weight of the composite enzyme is 8% of the total weight of Polygonatum odoratum, Arctium lappa and Echinacea purpurea, and the composite enzyme includes pectinase, acid protease, glucose oxidase, malate dehydrogenase and thermolysin in a mass ratio of 9:3:4:1:1, and performing enzymolysis for 6 hours to obtain an enzymolysis solution;

[0076] Pectinase was purchased from Shanghai Yuanye Biotechnology Co., Ltd., model number S10007.

[0077] Acidic protease was purchased from Shanghai Yuanye Biotechnology Co., Ltd., model number S10012.

[0078] Glucose oxidase was purchased from Shanghai Yuanye Biotechnology Co., Ltd., model number S10020.

[0079] Malate dehydrogenase was purchased from Shanghai Yuanye Biotechnology Co., Ltd., model number S10168.

[0080] Thermolysin was purchased from Shanghai Yuanye Biotechnology Co., Ltd., model number S10236.

[0081] (3) The enzymatic hydrolysate is concentrated, dried, and ultrafinely ground to obtain a composite enzymatic hydrolysate powder.

[0082] A method for preparing snow cherry fruit health tea comprises the following steps:

[0083] a) grinding and mixing the snow cherry fruit extract, aloe extract, American ginseng extract, composite fermentation powder and composite enzymatic hydrolysis powder in proportion to obtain a material;

[0084] b) adding water to the material and stirring at 110° C. for 3 h, wherein the amount of water added is 6 times that of the material, cooling to room temperature, standing for 8 h, and filtering to obtain a mixed solution;

[0085] c) the mixed solution is concentrated and dried to obtain the snow cherry health tea.

[0086] Example 2

[0087] The difference between this embodiment and embodiment 1 is that the formula of the snow cherry health tea and the preparation process of each raw material are different.

[0088] This embodiment provides a kind of snow cherry health tea, which comprises the following raw materials by weight:

[0089] Snow cherry extract 40 parts

[0090] 6 parts of aloe vera extract

[0091] 2 parts American ginseng extract

[0092] 11 parts of compound baking powder

[0093] 16 servings of compound enzymatic powder.

[0094] The preparation method of the snow cherry fruit extract is as follows:

[0095] (1) Pretreatment: fresh leaves were picked from snow cherry plants as raw materials, mixed with an 8% vitamin C aqueous solution to obtain a mixture, the mass ratio of the leaf raw material to the vitamin C aqueous solution was 1:8, the mixture was frozen at -5°C for 60 minutes, thawed at room temperature, a 3% sodium bicarbonate aqueous solution was added, the volume ratio of the mixture to the sodium bicarbonate aqueous solution was 1:0.02, heated in a water bath at 50-60°C for 5 hours, and filtered to obtain a pretreated solution;

[0096] (2) Resin treatment: the pretreatment solution is added to a macroporous adsorption resin column filled with ADS-800 grade adsorption resin. After filtering through the resin column, it is first eluted with 3 column volumes of 6% ethanol aqueous solution, and then eluted with 5 to 8 column volumes of 55% ethanol aqueous solution, and all the eluates are collected and combined;

[0097] (3) Concentration and drying: The eluate is concentrated under reduced pressure and dried to obtain the snow cherry fruit extract.

[0098] The preparation method of composite baking powder is:

[0099] (1) mixing the schisandra chinensis extract, the acanthopanax senticosus extract and the eucommia leaf extract in a proportion such that the mass ratio of the schisandra chinensis extract, the acanthopanax senticosus extract and the eucommia leaf extract is 3:8:1 to obtain a mixture;

[0100] (2) inoculating the mixture with 6 wt % of a composite fermentation agent, wherein the composite fermentation agent includes Clostridium butyricum, Bacillus coagulans and Aspergillus oryzae in a mass ratio of 1:2:6, continuously introducing air, fermenting at 28° C. and 70% humidity for 6 days, and filtering to obtain a fermentation liquid;

[0101] (3) Concentrating and drying the fermentation liquid to obtain composite fermentation powder.

[0102] The preparation method of the composite enzymatic hydrolysis powder is as follows:

[0103] (1) Washing polygonatum odoratum, burdock and echinacea, chopping them, air-drying them, drying them at 65°C for 1 hour, baking them at 160°C for 25 minutes, and ultrafine grinding them to obtain raw material powder; the mass ratio of polygonatum odoratum, burdock and echinacea is 5:1:3;

[0104] (2) adding water to the raw material powder in an amount of 9 times the weight of the raw material powder, mixing evenly, adjusting the pH value to 6, adding a composite enzyme at 35° C., wherein the added weight of the composite enzyme is 6% of the total weight of Polygonatum odoratum, Arctium lappa and Echinacea purpurea, and the composite enzyme includes pectinase, acid protease, glucose oxidase, malate dehydrogenase and thermolysin in a weight ratio of 9:3:4:1:1, and performing enzymolysis for 5 hours to obtain an enzymolysis solution;

[0105] (3) The enzymatic hydrolysate is concentrated, dried, and ultrafinely ground to obtain a composite enzymatic hydrolysate powder.

[0106] A method for preparing snow cherry fruit health tea comprises the following steps:

[0107] a) grinding and mixing the snow cherry fruit extract, aloe extract, American ginseng extract, composite fermentation powder and composite enzymatic hydrolysis powder in proportion to obtain a material;

[0108] b) adding water to the material and stirring at 100° C. for 4 h, wherein the amount of water added is 7 times that of the material, cooling to room temperature, standing for 6 h, and filtering to obtain a mixed solution;

[0109] c) the mixed solution is concentrated and dried to obtain the snow cherry health tea.

[0110] Comparative Example 1

[0111] The difference between this comparative example and Example 1 is that the added amount of the snow cherry fruit extract is 80 parts.

[0112] Comparative Example 2

[0113] The difference between this comparative example and Example 1 is that the macroporous resin column is filled with D101 resin purchased from Shanghai Hongshun Biotechnology Co., Ltd.

[0114] Comparative Example 3

[0115] The difference between this comparative example and Example 1 is that after filtering through the resin column, elution is performed with 10 times the column volume and a volume fraction of 60% ethanol aqueous solution.

[0116] Comparative Example 4

[0117] The difference between this comparative example and Example 1 is that the mass ratio of the Schisandra chinensis extract, the Acanthopanax senticosus extract and the Eucommia ulmoides leaf extract is 10:8:1.

[0118] Comparative Example 5

[0119] The difference between this comparative example and Example 1 is that the mass ratio of Clostridium butyricum, Bacillus coagulans and Aspergillus oryzae is 1:10:7.

[0120] Comparative Example 6

[0121] The difference between this comparative example and Example 1 is that Bacillus coagulans is replaced by Bacillus licheniformis, and the Bacillus licheniformis is purchased from Beijing Kezhan Biotechnology Co., Ltd., model number BNCC132665.

[0122] Comparative Example 7

[0123] The difference between this comparative example and Example 1 is that Clostridium butyricum is replaced by Clostridium tyrobutyricum, and Clostridium tyrobutyricum is purchased from Beijing Kezhan Biotechnology Co., Ltd., model number BNCC336696.

[0124] Comparative Example 8

[0125] The difference between this comparative example and Example 1 is that the mass ratio of Polygonatum odoratum, Arctium lappa and Echinacea purpurea is 6:5:3.

[0126] Comparative Example 9

[0127] The difference between this comparative example and Example 1 is that the mass ratio of pectinase, acid protease, glucose oxidase, malate dehydrogenase and thermolysin is 9:8:4:1:1.

[0128] Comparative Example 10

[0129] The difference between this comparative example and Example 1 is that the acidic protease is replaced by a neutral protease, and the neutral protease is purchased from Shanghai Yuanye Biotechnology Co., Ltd., model number S10013.

[0130] Performance Testing

[0131] (1) Sensory evaluation

[0132] The snow cherry health tea prepared in the embodiment and the comparative example was used for sensory evaluation. An evaluation team was composed of 15 professionals who received relevant training on project content and evaluation indicators, and sensory evaluation was performed according to the sensory indicator requirements of the product. The evaluation criteria are shown in Table 1. The main evaluation indicators are taste, color and flavor. The specific scoring criteria are as follows, and the final sensory evaluation score is the sum of the scores of each item.

[0133] 10 g of the snow cherry health tea prepared in the example and the comparative example was added with 150 mL of boiling water and brewed, and then cooled to a drinkable temperature before drinking. The evaluation results are shown in Table 2.

[0134] Table 1 Sensory evaluation standards

[0135]

[0136]

[0137] Table 2 Sensory evaluation results

[0138] Total evaluation score Example 1 95 Example 2 93 Comparative Example 1 88 Comparative Example 2 86 Comparative Example 3 83 Comparative Example 4 82 Comparative Example 5 80 Comparative Example 6 78 Comparative Example 7 76 Comparative Example 8 81 Comparative Example 9 84 Comparative Example 10 79

[0139] 15 g of the snow cherry health tea of ​​the embodiment and the comparative example was brewed with 150 mL of boiling water, cooled to room temperature, and the color was observed and scored. After being left at room temperature for 24 h, the color was observed and scored again. The scoring results are shown in Table 3.

[0140] Table 3 Stability test results

[0141] Color (after brewing) Color (after 24 hours) Example 1 27 24 Example 2 25 23 Comparative Example 1 22 16 Comparative Example 2 20 13 Comparative Example 3 21 15 Comparative Example 4 20 14 Comparative Example 5 21 15 Comparative Example 6 19 10 Comparative Example 7 20 11 Comparative Example 8 21 14 Comparative Example 9 22 15 Comparative Example 10 19 12

[0142] As can be seen from Table 3, the snow cherry health tea of ​​Examples 1-2 can still maintain its color after being placed at room temperature for 24 hours, and has good room temperature stability, while the color scores of Comparative Examples 1 to 10 all dropped significantly, indicating that the room temperature stability of Comparative Examples 1 to 10 is poor.

[0143] After brewing 15 g of the snow cherry health tea of ​​the examples and comparative examples with 150 mL of boiling water, the tea was cooled to room temperature and the color was observed. The tea was then placed at -20°C for 24 hours, returned to room temperature and the color of the tea was observed. It was found that the color of the tea of ​​Examples 1-2 was still a uniform reddish brown, while Comparative Examples 1 to 10 all darkened and became turbid to varying degrees, indicating that the snow cherry health tea prepared by the present invention has good stability at low temperatures.

[0144] After brewing 15 g of the snow cherry health tea of ​​the examples and comparative examples with 150 mL of boiling water, the tea was cooled to room temperature and the color was observed. The tea was then placed at 100° C. for 24 hours, returned to room temperature and the color of the tea was observed. It was found that the color of the tea of ​​Examples 1-2 was still a uniform reddish brown, while Comparative Examples 1 to 10 all darkened and became turbid to varying degrees, indicating that the snow cherry health tea prepared by the present invention also has good stability at high temperatures.

[0145] (2) Evaluation of immunity enhancement

[0146] ①Detection principle

[0147] Zebrafish is a small tropical freshwater fish native to South Asia. It has become a widely used vertebrate experimental animal worldwide because of its easy breeding, strong fertility, fast development, and transparent and easy-to-observe fertilized eggs. As a representative of lower vertebrates, zebrafish has 87% genetic homology with humans. It is now increasingly widely used in disease model creation, gene function evaluation, drug screening, toxicity testing, and environmental health risk assessment. At the same time, zebrafish experiments have also contributed to the research and development of traditional Chinese medicine, natural medicine, health food, and functional food. Humans and zebrafish are very similar in the cellular composition of the immune system, and the number of zebrafish neutrophils in samples with immune-regulating effects will also change accordingly.

[0148] ② Experimental instruments and reagents

[0149] Stereo microscope (SZX7, Olympus, Japan), chloramphenicol, berbamine hydrochloride

[0150] ③Detection system and sample size

[0151] Experimental system: Transgenic neutrophil fluorescent strain zebrafish

[0152] Zebrafish age: 3 days after fertilization

[0153] Sample size per group: 30 strips per well, 1 well per group

[0154] Adult fish rearing and breeding methods: According to standard rearing and breeding methods.

[0155] ④ Experimental process

[0156] The zebrafish were divided into 15 groups, namely a blank control group, a model control group, a positive control group and a sample treatment group, wherein the sample treatment group included the snow cherry health tea of ​​Examples 1-2 and Comparative Examples 1-10. The test substance stock solution was diluted in an isotropic manner with the aid of buffered water to form a set of gradient solutions of appropriate concentrations, with a dilution multiple of not less than 10 times, and kept for later use.

[0157] Among them, the blank control group was not treated, chloramphenicol was added to the model control group, and berbamine hydrochloride and the sample solution to be tested (the snow cherry health tea prepared in the embodiment and the comparative example) were added to the positive control group and the sample treatment group while chloramphenicol was added. After a period of cultivation, the fluorescence intensity of the neutrophils of the zebrafish was detected.

[0158] ⑤Data processing method

[0159] SPSS26 software was used for analysis, GraphPad Prism8 was used for drawing, and data statistics were expressed as mean ± standard deviation. SPSS software was used for normal distribution test of experimental results. If the data conformed to normal distribution, T parameter test or analysis of variance (ANOVA) was used for significance. If it did not conform to normal distribution, non-parametric test was used (result P value < 0.05 indicated statistically significant difference)

[0160] ⑥Judgment basis

[0161] On the basis of the effectiveness of the test, the model control group was compared with the blank control group, and the number of neutrophils (or fluorescence intensity) in the model control group was significantly reduced and P < 0.05, which means that the modeling was successful. The sample treatment group was compared with the model control group, and the number of neutrophils (or fluorescence intensity) in the sample treatment group was significantly increased and P < 0.05, indicating that the sample has the effect of enhancing immunity.

[0162] ⑦Preliminary experimental results

[0163] There is no significant difference between the sample treatment group with 1% concentration and the blank control group, so 1% concentration is the maximum safe concentration. The test results are shown in Table 4-5.

[0164] Table 4 Test results of blank control group, model control group, positive control group and treatment group of Example 1

[0165]

[0166] Figure 1 and Figure 2 The fluorescence intensity comparison diagram and phenotype diagram of the blank control group, model control group, positive control group and sample treatment group of Example 1 are shown respectively. Figure 1 As can be seen from Table 4, the fluorescence intensity of the sample treatment group in Example 1 is significantly higher than that of the model control group, and the fluorescence intensity of Example 1 is also higher than that of the positive control group, indicating that the snow cherry health tea prepared in Example 1 has an excellent effect of enhancing immunity. Figure 2 It can be seen that the phenotype diagram of Example 1 has bright fluorescence, indicating that its effect of enhancing immunity is very significant.

[0167] Table 5 Test results of the immune enhancement efficacy of the embodiments and comparative examples

[0168]

[0169] The 1% snow cherry health tea prepared in the example and comparative example was placed at -20°C and 100°C for 20 hours respectively, restored to room temperature, and zebrafish animal experiments were conducted again to evaluate the temperature stability of the snow cherry health tea. The test results are shown in Table 6.

[0170] Table 6 Stability test results

[0171]

[0172]

[0173] It can be seen from the above performance test results that the sensory evaluation, stability and immunity-enhancing effects of Examples 1-2 are good, especially the comprehensive performance of Example 1 is the most outstanding. This is mainly because the preparation process of the snow cherry fruit extract has been designed, and the overall formula uses composite fermentation powder and composite enzymatic hydrolysis powder. The synergistic effect of multiple substances improves the solubility and health-care effects of the snow cherry fruit health tea.

[0174] The comparative example, because it did not adopt the necessary technical solutions, was significantly worse than the embodiment in the corresponding performance test. In comparative example 1, the amount of the snow cherry extract was changed. It can be seen that the stability of the health tea and the effect of enhancing immunity decreased, proving that the amount of the snow cherry extract added has an important influence on the comprehensive effect of the health tea. Each raw material has a synergistic effect with other raw materials. Once the amount of one raw material added increases, it will affect the synergistic effect and reduce the sensory and efficacy of the health tea. The type of resin in the macroporous resin column was changed in comparative example 2. From the results, it can be seen that the resin type affects the effective ingredients in the snow cherry extract, and then affects the comprehensive performance of the health tea, indicating that the resin type has an important influence on improving the comprehensive performance of the health tea. Comparative example 3 changes the resin elution method, using a concentration of ethanol for elution, which will also affect the effective ingredients of the snow cherry extract, and then affect the comprehensive performance of the entire snow cherry health tea product. Comparative Examples 4 to 5 respectively changed the mass ratio of the three extracts and the mass ratio of the three fermentation strains in the preparation process of the composite fermentation powder. It can be seen from the results that the stability of the prepared snow cherry health tea and the effect of enhancing immunity decreased to varying degrees, indicating that the mass ratio of the three extracts and the mass ratio of the three fermentation strains have a very important influence on the performance of the health tea. Comparative Examples 6 to 7 changed the type of one of the three fermentation strains. It can be seen from the results that the stability of the prepared health tea and the effect of enhancing immunity decreased further than Comparative Examples 4 to 5, indicating that the type of fermentation agent directly affects the composition of the composite fermentation powder, thereby affecting the efficacy and sensory evaluation of the health tea. Comparative Examples 8 to 9 respectively changed the mass ratio of Polygonatum odoratum, Burdock and Echinacea in the composite enzymatic hydrolysis powder and the mass ratio of several enzymes. It can be seen from the results that the comprehensive performance of the prepared health tea deteriorated, indicating that the above mass ratio directly affects the effective ingredients of the composite enzymatic hydrolysis powder, and the mass ratio of the above raw materials and several enzymes plays an important role in improving the comprehensive performance of the health tea. Comparative Example 10 changes the type of enzyme, replacing the acidic protease with a neutral protease. From the results, it can be seen that the comprehensive performance of the prepared health tea is further reduced compared with Comparative Example 9, indicating that the type of enzyme has an important influence on the comprehensive performance of the health tea. The above experimental results further prove the importance of the technical solution defined in the present invention for its technical effect.

[0175] Therefore, the present invention adopts a snow cherry health tea with the above structure and a preparation method thereof. The prepared snow cherry health tea not only improves the solubility and bioavailability of the product, but also enhances its stability and functionality, so that the product has significant advantages in improving immunity. Through these carefully designed process steps, the product can better meet consumers' needs for health care.

[0176] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that they can still modify or replace the technical solution of the present invention with equivalents, and these modifications or equivalent replacements cannot cause the modified technical solution to deviate from the spirit and scope of the technical solution of the present invention.

Claims

1. A snow cherry fruit health tea, characterized in that: The following raw materials are included in parts by weight: 40-60 parts of Snow Cherry Extract 5-10 parts of aloe vera extract 1-5 portions of American ginseng extract 10-15 parts of compound baking powder 15-20 portions of compound enzymatic hydrolysis powder; The composite fermentation powder is obtained by fermenting and drying schisandra chinensis extract, acanthopanax senticosus extract and eucommia leaf extract through a composite fermentation agent; the mass ratio of schisandra chinensis extract, acanthopanax senticosus extract and eucommia leaf extract is (3-5):(7-10):1; the composite fermentation agent includes Clostridium butyricum, Bacillus coagulans and Aspergillus oryzae in a mass ratio of 1:(2-4):(6-8); The composite enzymatic hydrolysis powder comprises enzymatic hydrolysis powder of polygonatum odoratum, enzymatic hydrolysis powder of burdock and enzymatic hydrolysis powder of echinacea. The enzymatic hydrolysis powder of polygonatum odoratum, enzymatic hydrolysis powder of burdock and enzymatic hydrolysis powder of echinacea are obtained by mixing polygonatum odoratum, burdock and echinacea and then enzymolyzing them with composite enzyme. The added mass of composite enzyme is 5-10% of the total mass of polygonatum odoratum, burdock and echinacea, and the mass ratio of polygonatum odoratum, burdock and echinacea is (5-8):1:(2-4); the composite enzyme comprises pectinase, acid protease, glucose oxidase, malate dehydrogenase and thermophilic protease according to the mass ratio of (8-10):(2-4):(3-5):1:

1.

2. The snow cherry health tea according to claim 1, characterized in that: The preparation method of the snow cherry fruit extract is as follows: (1) Pretreatment: fresh leaves are picked from snow cherry plants as raw materials, mixed with an 8-10% vitamin C aqueous solution to obtain a mixture, the mixture is placed at -10-5°C for 60-90 minutes, thawed at room temperature, a 3-5% sodium bicarbonate aqueous solution is added, heated in a water bath at 50-60°C for 5-10 hours, and filtered to obtain a pretreated solution; (2) Resin treatment: Add the pre-treated solution to a loaded macroporous adsorption resin column, filter through the resin column, elute with ethanol-water solution, and collect and combine all the eluates; (3) Concentration and drying: The eluate is concentrated under reduced pressure and dried to obtain the snow cherry fruit extract.

3. The snow cherry health tea according to claim 2, characterized in that: The mass ratio of the leaf raw material to the vitamin C aqueous solution is 1:8-10.

4. The snow cherry health tea according to claim 2, characterized in that: The volume ratio of the mixture to the sodium bicarbonate aqueous solution is 1:0.01-0.

05.

5. The snow cherry health tea according to claim 2, characterized in that: The macroporous adsorption resin column is packed with ADS-800 grade adsorption resin.

6. The snow cherry health tea according to claim 2, characterized in that: The specific elution process of the resin column is: firstly use 1 to 3 times the volume of the column with a volume fraction of 5 to 8% ethanol aqueous solution for elution, and then use 5 to 8 times the volume of the column with a volume fraction of 50 to 65% ethanol aqueous solution.

7. The snow cherry health tea according to claim 1, characterized in that: The preparation method of composite baking powder is: (1) mixing the Schisandra chinensis extract, the Acanthopanax senticosus extract and the Eucommia ulmoides leaf extract in proportion to obtain a mixture; (2) inoculating 6 to 8 wt% of a composite fermentation agent into the mixture, continuously introducing air, fermenting at 25 to 35° C. and 50 to 75% humidity for 5 to 10 days, and filtering to obtain a fermentation liquid; (3) Concentrating and drying the fermentation liquid to obtain composite fermentation powder.

8. The snow cherry health tea according to claim 1, characterized in that: The preparation method of the composite enzymatic hydrolysis powder is as follows: (1) Washing polygonatum odoratum, burdock and echinacea, chopping them, air-drying them, drying them at 55-65° C. for 1-3 hours, baking them at 160-180° C. for 20-40 minutes, and ultrafine grinding them to obtain raw material powder; (2) adding water to the raw material powder in an amount of 8 to 12 times the mass of the raw material powder, mixing evenly, adjusting the pH value to 6 to 7, adding a composite enzyme at 30 to 45° C., and performing enzymolysis for 5 to 8 hours to obtain an enzymolysis solution; (3) The enzymatic hydrolysate is concentrated, dried, and ultrafinely ground to obtain a composite enzymatic hydrolysate powder.

9. The method for preparing the snow cherry health tea according to any one of claims 1 to 8, characterized in that: The following steps are involved: a) grinding and mixing the snow cherry fruit extract, aloe extract, American ginseng extract, composite fermentation powder and composite enzymatic hydrolysis powder in proportion to obtain a material; b) adding water to the material and stirring at 100-120° C. for 2-5 hours, wherein the amount of water added is 5-8 times of the material, cooling to room temperature, standing for 5-10 hours, and filtering to obtain a mixed solution; c) the mixed solution is concentrated and dried to obtain the snow cherry fruit health tea.

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