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Calcium carbonate producing actinomycetes and application thereof

A technology of actinomycetes and calcium carbonate, which is applied in the restoration of bacteria and contaminated soil, water/sludge/sewage treatment, etc., can solve the problems of non-degradable and difficult treatment, achieve strong tolerance and improve alkalinity Effect

Inactive Publication Date: 2017-05-10
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The problem of heavy metal pollution has become an important environmental problem that threatens the survival and development of human beings, and it is relatively stable in the environment and cannot be degraded, making it very difficult to treat

Method used

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  • Calcium carbonate producing actinomycetes and application thereof
  • Calcium carbonate producing actinomycetes and application thereof
  • Calcium carbonate producing actinomycetes and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1: Isolation and identification of the center bacterial strain used in the present invention

[0031] After the sediment samples taken from the Yellow Sea of ​​China were serially diluted with normal saline, 100uL of the suspension was coated with solid 2216E medium, cultured at 28°C for 24 hours, single clones were picked, and re-stretched on the 2216E plate for separation and purification. After the purified single clone is obtained, the strain is preserved. The obtained purified strain was first tested positive for urease with 9219 medium (peptone 1g, potassium dihydrogen phosphate 2g, sodium chloride 5g, urea 20g, glucose 1g, pink 0.012g, agar powder 15g, old seawater 1000ml, pH 6.8±0.2) The screening of bacterial strain, the bacterial strain that screens out is further used to produce calcium carbonate medium (nutrient broth 3g, sodium bicarbonate 2.12g, ammonium chloride 10g, anhydrous calcium chloride 16.65g, urea 20g, agar powder 15g, old seawater 100...

Embodiment 2

[0032] Example 2: Ability of bacterial strain BS258 to precipitate calcium carbonate and tolerance to calcium ions

[0033]The morphology and ability of the strain BS258 precipitated calcium carbonate were identified. Select 9219-base liquid medium (peptone 10g, yeast powder 5g, anhydrous calcium chloride 3.33g, sodium acetate 2g, potassium dihydrogen phosphate 2g, nickel sulfate 0.005g, manganese sulfate 0.005g, sodium chloride 5g, urea 5g , glucose 20g, old seawater 1000ml) induced BS258 to precipitate calcium carbonate, and after cultivating at 28°C and 160rpm for 5 days, the precipitated calcium carbonate was collected for optical microscope, scanning electron microscope and transmission electron microscope observation, as figure 2 As shown, calcium carbonate particles precipitated by BS258, bacterial cell imprints left on larger calcium carbonate particles, and bacterial cells encapsulated by calcium carbonate can be observed. At the same time, the pH change of the cult...

Embodiment 3

[0035] Example 3: Ability of strain BS258 to co-precipitate heavy metals

[0036] Add 5mM different metal ions (Cu 2+ ,Zn 2+ ,Mn 2+ ,Sr 2 + ), inoculate the BS258 seed solution at a volume ratio of 1:100, and at 28° C., after cultivating at 160 rpm for 5 days, collect the precipitate and carry out scanning electron microscope-X-ray energy spectrum analysis, such as Figure 5 As shown, in addition to carbon, oxygen, and calcium elements, the sediment also contains corresponding heavy metal elements, indicating that heavy metal ions in the solution can co-precipitate with calcium carbonate, so as to achieve the purpose of remediation of heavy metal pollution. At the same time, the coprecipitation of the BS258 bacterial strain was measured to remove heavy metal ions. As shown in Table 1 below, BS258 had the strongest removal ability for manganese ions and strontium ions in the solution, and more than 99% of the manganese ions and strontium ions were Calcium carbonate was co-...

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Abstract

The invention belongs to the technical field of functional microbiological screening and application, and particularly relates to calcium carbonate producing actinomycetes and application thereof. The calcium carbonate producing actinomycetes is brevibacterium linens BS258, a strain is preserved in the Common Microorganism Center of the China Committee for Culture Collection of Microorganisms in Number 3 (the Institute of Microbiology) of the Number 1 courtyard on the West Beichen Road in the Chaoyang District of Beijing on September 26th, 2016, a preservation number of the strain is CGMCC 1.15904, and a taxonomy name of the strain is brevibacterium linens BS258. The calcium carbonate producing actinomycetes and the application have the advantages that calcium carbonate precipitates can be induced by the actinomycetes, and the strain can tolerate high-calcium-ion environments and can resist diversified heavy metal ions; calcium ions in the environments can be induced under the effect of carbonic anhydrase by the aid of alkaline micro-environments generated in urea decomposition procedures to form the calcium carbonate precipitates, the heavy metal ions can be co-precipitated in calcium carbonate forming procedures, accordingly, the purposes of biological mineralization and heavy metal restoration can be achieved, and the shortcomings in the prior art can be overcome.

Description

technical field [0001] The invention belongs to the technical field of functional microorganism screening and application, and in particular relates to a calcium carbonate-producing actinomycete and its application. Background technique [0002] Accidents of cracking and failure of concrete structures have occurred frequently at home and abroad, causing serious damage to related historical relics and buildings, bringing huge economic losses to the country and people, and seriously threatening personal safety. At present, the repair methods for concrete cracks mainly include grouting repair, electrochemical repair and bionic self-repair, etc. The microbial repair technology using biomineralization is a kind of bionic self-repair. Since then, it has become a research hotspot, and there have been related applications of filling microbes and nutrients into concrete cracks for repair abroad. [0003] Heavy metal pollution has become an important environmental problem that threat...

Claims

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Application Information

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IPC IPC(8): C12N1/20C04B24/00C02F3/34C02F11/02B01D53/84B01D53/62B09C1/10C12R1/13C02F101/20
CPCB01D53/62B01D53/84B09C1/10C02F3/34C02F11/02C02F2101/20C04B24/00C04B2103/0001C12N1/205C12R2001/13Y02P20/59Y02A50/20
Inventor 孙超岷朱玉莹马宁
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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