Efficient anaerobic antimony reduction bacterial strain and application thereof

A technology of antimony trioxide and campylobacter, applied in the field of environmental biology, can solve problems such as not being widely studied, and achieve the effect of broad application prospects

Active Publication Date: 2020-05-15
UNIV OF SCI & TECH OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among them, microorganisms play a vital role in the biochemical cycle of trace elements in soil, but the role of soil microorganisms in the migration and transformation of antimony pollutants has not been widely studied.

Method used

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  • Efficient anaerobic antimony reduction bacterial strain and application thereof
  • Efficient anaerobic antimony reduction bacterial strain and application thereof
  • Efficient anaerobic antimony reduction bacterial strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1, isolation, screening and identification of bacterial strains

[0043] (1) Material preparation

[0044] 1. The soil used in the experiment was taken from the antimony polluted area in Lengshuijiang City, Hunan Province.

[0045] 2. Medium

[0046] Anaerobic Mineral Salt Medium (BSM): K 2 CO 3 1.5 H 2 O, 0.24g / L; KH 2 PO 4 ,0.1g / L; K 2 HPO 4 , 0.15g / L; NH 4 Cl, 0.075g / L, pH 7.0 trace element mixture 10mL / L, 14mmol / L MgCl 2 with 700μmol / L CaCl 2 The mixed solution of 1ml / L, sodium lactate 20mmol / L. Use N 2 / CO 2 (vol / vol 80 / 20) was aerated for 15 minutes, packaged in serum bottles, and sterilized under high temperature and high pressure.

[0047] Anaerobic LB liquid medium: peptone 10.0g / L, yeast extract 5.0g / L, sodium chloride 10.0g / L. After deoxygenation with high-purity nitrogen, high temperature and high pressure sterilization.

[0048] 3. Potassium pyroantimonate mother liquor configuration:

[0049] Take 2g of potassium pyroantimonate ...

Embodiment 2

[0058] Embodiment 2, the growth situation of fermenting clay bent bacteria (Pelosinus fermentans) Ti_20 under different culture conditions

[0059] Curvularia clay fermentum Ti_20 was inoculated in BSM medium containing different electron donors and cultured anaerobically at 30°C. A total of 3 experimental groups: (1) anaerobic BSM medium + 20mM sodium lactate (Lactate); (2) anaerobic BSM medium + 40mM potassium fumarate (Fumarate); (3) anaerobic BSM medium + 20mM sodium lactate (Lactate)+40mM potassium fumarate (Fumarate). To compare the anaerobic growth curves of fermenting Campylobacter clay Ti_20 under different carbon source conditions.

[0060] Figure 4 The results showed that compared with rapid anaerobic fermentation of potassium fumarate, the bacterium had a lower utilization rate of lactic acid, but it could still slowly ferment lactic acid. When sodium lactate and potassium fumarate coexisted, the growth state of the bacteria was the best.

Embodiment 3

[0061] Embodiment 3, the reduction of Sb (V) to Sb (V) by fermenting clay bent bacteria (Pelosinus fermentans) Ti_20

[0062] Biotoxicity of pentavalent antimony to Campylobacter fermentum Ti_20: inoculate Campylobacter fermentum Ti_20 in anaerobic LB, and add 2mM pentavalent antimony to the medium, culture it anaerobically at 30℃ for 2 days, and take the bacterial liquid Centrifuge (6000rpm, 5min) to remove the supernatant. The collected bacteria were fixed with 2.5% glutaraldehyde at 4°C for 24 hours, then dehydrated with 50%, 70%, 80%, 90%, and 100% ethanol in gradient, and finally the dehydrated bacteria were used for scanning electron microscopy The shooting of the picture. The results showed that the bacteria lengthened significantly after adding antimony, indicating that the biotoxicity of antimony would seriously inhibit bacterial division ( Figure 5 ).

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Abstract

The invention belongs to the technical field of environment biologics and discloses an efficient anaerobic antimony reduction bacterial strain and application thereof. The efficient anaerobic antimonyreduction bacterial strain is separated from soil of an antimony polluted region of the Lengshuijiang city in Hunan province in China, 16S#rDNA verification shows that the strain belongs to pelosinusfermentans, is named as Pelosinus fermentans Ti_20, and is preserved in the Guangdong Microbe Culture Collection Center, and the preservation number is GDMCC No:60921. The Pelosinus fermentans Ti_20not only has a capability of efficiently reducing pentavalent antimony, but also is capable of converting trivalent antimony at a reduction state into Sb2O3 precipitate, and biological mineralizationof antimony can be achieved. The strain has wide application prospects in environment repairing of an antimony polluted mine region. Formed antimonous oxide can be also used as a flame retardant withexcellent performance or an electrode replacement material of a lithium or sodium ion battery.

Description

technical field [0001] The invention belongs to the technical field of environmental biology, and in particular relates to a high-efficiency anaerobic antimony-reducing bacterial strain and application thereof. Background technique [0002] As a toxic metal pollutant of global concern, although antimony (Sb) exists in the natural geological environment in trace concentrations, human activities such as mining, smelting, and military training have released a large amount of antimony into water, soil, and sediment, resulting in severe environmental pollution. Antimony is the ninth most mined metal element in the world. China has the world's largest antimony mine (Hunan Tin Mine), and its antimony output accounts for about 80% of the world's antimony output. According to reports, the concentration of antimony in my country's polluted sediments can reach 1163mg / kg, far exceeding the environmental background value. At the same time, antimony and its compounds are widely used in ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P3/00C12R1/01
CPCC12N1/20C12P3/00C12N1/205C12R2001/01
Inventor 俞汉青闵迪刘东风
Owner UNIV OF SCI & TECH OF CHINA
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