4 results about "Mandelonitrile" patented technology

The application discloses a mandelonitrile-degrading bacterium SYY40 and a culture method and application thereof, relates to the technical field of microbial pollution degradation and environmental pollution, and provides the mandelonitrile-degrading bacterium SYY40 which is identified as Pseudomonas sp. SYY40 and named Pseudomonas sp. SYY40, and is preserved in the China General Microbiological Culture Collection Center on May 19, 2025, and the preservation number of the strain is CGMCC NO. 34596. The strain can realize efficient removal of mandelonitrile, and the degradation rate of the mandelonitrile reaches 100% within 48 hours.

The invention discloses a nitrilase mutant with high activity and stereoselectivity and application of the nitrilase mutant in synthesis of chiral carboxylic acid. The mutant is obtained by performing single mutation or multiple mutation on 79th, 166th, 167th and 246th amino acids of an amino acid sequence shown as SEQ ID NO.1. The invention further discloses a preparation method of the nitrilase mutant. According to the invention, the nitrilase OsNIT-mut is subjected to molecular modification through (semi) rational design, and a mutant with synergistically improved hydrolysis reaction specificity and stereoselectivity is obtained. The content of mandelic acid in a product of the mutant OsNIT-mut / V246N / T166G / R79Y / A167N for catalyzing mandelonitrile is 90.5%, the e.e. Value of R-mandelic acid is increased to 95.2%, and the relative hydrolytic activity is 399.1%. The nitrilase mutant can be applied to green industrial catalytic synthesis of chiral mandelic acid through regulation and control of hydrolysis reaction specificity and stereoselectivity, and has important significance.

The invention discloses a nitrilase mutant with high stereoselectivity and hydration activity and application of the nitrilase mutant. The mutant is obtained by performing single mutation or multiple mutation on amino acids at the 163rd, 166th and 201st sites of an amino acid sequence shown as SEQ ID NO.1. The invention further discloses a preparation method of the nitrilase mutant. According to the invention, molecular modification is carried out on reaction non-specific nitrilase OsNIT through (semi) rational design, so that a mutant with strict hydration activity and high stereoselectivity is obtained. When the mutant OsNIT-M0 / L163E / T166I / M201L is used for catalyzing mandelonitrile, the content of mandelamide is changed into 96.8%, the e.e. Value of R-mandelamide is increased to 92.7%, and the relative hydration activity is 163% of that before mutation. The reaction specificity and stereoselectivity of the nitrilase are regulated and controlled, so that the nitrilase can be applied to green industrial catalytic synthesis of amide and has important significance.

The invention provides a method for preparing adiponitrile by using 1, 4-butanediol, which comprises the following steps: under the protection of inert gas, sequentially adding 1, 4-butanediol, sodium borohydride and iodine into a dioxane solution by using an aminopyrrole aluminum lithium bimetallic compound {[C4H3N (AlEt3) (2-CH2NHLitBu)] [C4H3NLi (2-CH2NHtBu)]} 2 as a catalyst, heating to 50 DEG C, stirring to react for 5-6 hours, and filtering to obtain the adiponitrile. And adding mandelonitrile and cesium carbonate, continuously stirring for 5-6 hours, quenching after the reaction is finished, filtering, and concentrating filtrate in vacuum to obtain the target product adiponitrile. The method disclosed by the invention does not need the participation of a highly toxic reagent hydrogen cyanide, is green and environment-friendly, wide in raw material source, low in cost and simple and convenient in post-treatment operation, and has important significance for extending the industrial chain of 1, 4-butanediol and solving the adverse situation of excessive production capacity in China.