36 results about "Pseudomonas pseudoalcaligenes" patented technology

The invention discloses a method for treating heavy metal wastewater. The method comprises the following steps: (1) immobilizing mixed bacteria of pseudomonas pseudoalcaligenes and saccharomyces cerevisiae, collected after mixed fermentation culture, into immobilized cell spheres, wherein the appearances of the immobilized cell spheres are spherical, and the interiors of the immobilized cell spheres are of a porous mesh structure; (2) adding a rhizopus oryzae spore suspension liquid into starch bearing material hydrolysate as a culture medium at the pH of 3-6, carrying out shake cultivation for 24-48 hours, filtering to collect rhizopus oryzae mycelium spheres with the diameters of 50-100 microns; (3) filling a biological adsorber with the immobilized cell spheres and the rhizopus oryzae mycelium spheres, wherein the biological adsorber is formed by virtue of series connection of multiple adsorption columns; and (4) after regulating the initial heavy metal ion concentration of heavy metal wastewater to be less than or equal to 800mg/L and the pH value to be 5-7, enabling heavy metal wastewater to flow through the biological adsorber, wherein the hydraulic retention time in each adsorption column is 0.5h-1h. According to the method, different heavy metals (Cd<2+>, Hg<2+>, Cu<2+>, Zn<2+>, Ni<2+>, Ag<2+> and Pb<2+>) can be well adsorbed by virtue of different types of microbial live bacteria under different adsorption mechanisms.

The invention relates to a denitrifying phosphate-accumulating organism with nitrogen and phosphorus removal functions and applications thereof. The strain is named as pseudomonas pseudoalcaligenes CL-1, belongs to pseudomonas pseudoalcaligenes, and is preserved in China Center for Type Culture Collection on June 13, 2012, and the preservation numbers is CCTCC NO: M2012225. The strain is gram-positive and belongs to brevibacterium, a bacterial colony is rounded, milk white, convex and glossy, and edges are smooth and transparent. The growth cycle of the organism is long, the nitrogen and phosphorus removal rates in the first 24h are respectively 56.93% and 43.56%, and the nitrogen and phosphorus removal rates in the whole growth cycle are respectively 75.6% and 57.5%. The strain has a complete denitrifying capacity, and can directly reduce inorganic nitrogen such as nitrate nitrogen and nitrite nitrogen and the like to harmless nitrogen gas discharged from water bodies. When the denitrifying phosphate-accumulating organism is used for treating nitrogen and phosphorus containing wastewater, the process is simple, the treatment effect is efficient and stable, and the operating cost is saved, meanwhile, no greenhouse gas such as NO and N2O and the like is produced, therefore, no air pollution is caused.

The invention discloses a waste water treatment agent. The waste water treatment agent is prepared by using the following steps: 1, mixed fermenting, culturing and collecting pseudomonas pseudoalcaligenes and saccharomyces cerevisiae thalli; 2, mixing polyvinyl alcohol, calcium alginate, acrylyl and water according to a mass ratio of (6-15):(0.5-1.5):(3-5):100 to obtain a polyvinyl alcohol hydrosol, and adding the collected pseudomonas pseudoalcaligenes and saccharomyces cerevisiae thalli into the polyvinyl alcohol hydrosol to form a mixed solution; 3, adding the mixed solution into a saturated boric acid solution containing N'-methylene bisacrylamide CaSO4 by using a peristaltic pump to form a gel bead, cooling and unfreezing the gel bead, then cooling and unfreezing the gel bead again, placing in phosphate with the mass concentration of 0.2-0.5 g/L to obtain an immobilized cell bead with a spherical appearance and an internal porous mesh structure. The invention also discloses a method for treating waste water by using the treatment agent. Different heavy metals are well adsorbed.

The invention provides a bacteria strain having a high efficiency broad spectrum degradation ability for an organophosphorus pesticide, the strain belongs to Pseudomonas pseudoalcaligenes, and is named as C2-1, the invention also provides a seperation process, cultivation conditions, and a degradation characteristic of the strain, the strain can be grew on an inorganic salt culture medium by using the pesticide. Also provided is seperation and purification of an organophosphorus pesticide degradation enzyme excreted by the strain, and study on enzyme properties, and seperation and clone of genes coding the enzyme. The invention provides a good gene source for cloning organophosphorus pesticide degradation enzyme genes by using a gene project means, finally realizes an object of producing the organophosphorus pesticide degradation enzyme.

The invention provides a pseudomonas pseudoalcaligenes new bacterial strain MOB13, the preserving number of the strain is CGMCC No.2763, and the new bacterial strain can be used for producing IAA and prolease which are related to antibacterium. The new bacterial strain has plate inhibiting effect on parts of plant pathogenic fungis (such as ceratocystis fimbriata, fusarium.oxysporum f.sp.niveum, monilinia laxa and phytophthora capsici). Plate, potted plant and field experiment are used for testing the control effect of Pseudomonad MOB13 to Rhizoctonia cerealis, and the control efficiency is respectively 62.13% and 73.87%. The new bacterial strain MOB13 has good growth promotion effect for wheat, can promote the plant height of wheat to increase 34.97%, and leads the root length to increase 17.34%.

The invention discloses a compound microorganism microsphere and a preparation method thereof, and belongs to the technical field of microorganisms in sewage treatment. The method comprises the following steps that (1) pseudomonas mendocina and pseudomonas pseudoalcaligenes are subjected to primary embedding and crosslinked immobilization; (2) the product obtained through primary crosslinked immobilization in the first step and bacillus subtilis are mixed and are then subjected to secondary embedding and crosslinked immobilization; the compound microorganism microsphere is obtained. Two layers of microorganisms are embedded in the compound microorganism microsphere prepared by the preparation method; the inner layer contains the pseudomonas mendocina and the pseudomonas pseudoalcaligenes; the outer layer contains the bacillus subtilis. The embedding method is used twice, so that the microorganism immobilization is firmer; the bacteria density is increased; the sewage treatment efficiency is improved; the magnet powder has strong adsorption force; the crosslinking effect among mixed microorganisms is enhanced; the immobility of mixed bacteria is improved.

The invention discloses a mixed bacterial colony microbial preparation which is a bacterial suspension consisting of paenibacillus, pseudomonas stutzeri, pseudomonas pseudoalcaligenes and pseudomonas oleovoran, wherein the viable count of paenibacillus is more than 1.69*10<10>CFU/Ml, the viable count of pseudomonas stutzeri is more than 2.35*10<10>CFU/Ml, the viable count of pseudomonas pseudoalcaligenes is more than 2.18*10<10>CFU/Ml, and the viable count of pseudomonas oleovoran is more than 2.26*10<10>CFU/Ml. The mixed bacterial colony microbial preparation can be used for treating wastewater containing nitrate nitrogen, and the specific application method comprises the steps of regulating the pH value of the wastewater containing nitrate nitrogen to 7.0-7.2, then inoculating the wastewater containing nitrate nitrogen with the mixed bacterial colony microbial preparation according to the inoculation amount of 1-10%, and performing static culture at 30-35 DEG C. The denitrification effect can be obvious after 4 days culture and complete denitrification can be realized.

The present invention provides a kind of new pesticidal protein gene and the gene coded protein. The pesticidal protein gene is originated from Pseudomonas pseudoalcalligenes, and its coded protein has wide and powerful intoxicating effect on grasshopper and Orthoptera insects, so that the pesticidal protein gene may be used in preventing and controlling pest of crop. The present invention also provides the Pseudomonas pseudoalcalligenes pesticidal protein separating and purifying method, and one method of producing pesticidal transgenic plant.

The invention relates to a microbial treatment method for domestic garbage. A biological treatment agent containing bacillus cereus, lactobacillus acidophilus, bacillus stearothermophilus, pseudomonas pseudoalcaligenes and saccharomyces cerevisiae is added with water and then is subjected to mixed fermentation with the garbage, so that the objective of degrading the garbage is achieved, and the degradation rate is up to 85%. The method is simple in operation and easy to master, moreover, has good effect on degradation of the garbage, and is beneficial for rapid treatment of the garbage and environmental protection.

The invention relates to the field of microbial technology, and particularly provides a microbial agent capable of degrading garbage. Domestic garbage, especially kitchen waste, can be efficiently degraded through the synergetic promoting effect of bacillus cereus, lactobacillus acidophilus, bacillus stearothermophilus, pseudomonas pseudoalcaligenes and saccharomyces cerevisiae, the degradation rate can achieve 80%, the living environment can be beneficially improved, and the environmental pollution can be alleviated.

The invention discloses a low-pollution-water-body remediation microbial community. The microbial community is prepared from light gordona, asphalt pseudo-burkholderia, sphingomonas, stenotrophomonas rhizophila, pseudomonas pseudoalcaligenes and azospirillum. The low-pollution water-body repairing microbial community has the advantages that by means of the obtained microbial community, treatment efficiency of a low-pollution water body is high, and factors of the sludge yield are small.

The invention discloses a complex microbial inoculant for wastewater treatment and a preparation method thereof.The complex microbial inoculant comprises shewanella putrefaciens, pseudomonas pseudoalcaligenes and modified zeolite.Chitosan is introduced to the zeolite, the adsorption performance of the zeolite is improved, then composite modification is conducted, iron phosphate is introduced to the zeolite, and the adsorption performance of the zeolite is improved; the iron ions can be used as a coagulant and cooperate with chitosan to flocculate and settle ammonia nitrogen substances in sewage, the biological nitrification performance of the composite microbial inoculant is greatly improved, in addition, the iron ions can increase the content of organic polymers in bacterial biofilms, and the colloidal network structure of the organic polymers can aggregate cells together, so that the biological nitrification performance of the composite microbial inoculant is improved. Meanwhile, the biological membrane is adhered to the surface of the zeolite carrier, so that the connectivity between the zeolite carrier and microorganisms is increased, the leakage of the microorganisms is effectively prevented, and the complex microbial inoculant can keep a stable biological nitrification effect.

The present invention provides new promoter sequence of Pseudomonas pseudoalcaligenes. The present invention includes gene constituent containing the promoter sequence of the present invention, and the promoter sequence is connected to neomycin phosphotransferase gene sequence encoding the structure gene optionally. The present invention also provides the carrier expressing structure gene encoded product of gene constituent in the present invention and the host cell as well as the cell transformed by the heterologous gene connected to the promoter optionally.

The invention discloses a pseudomonas pseudoalcaligenes and an application thereof. The pseudomonas pseudoalcaligenes FX 13 is screened from bottom mud of a penaeus vannmei culture pond, and the conservation number thereof is CCTCC NO: M2013069. The strain has aerobic and denitrification properties, and has an efficient degradation effect on nitrate and nitrite in water environment.

The invention relates to a method for producing a microbial agent for processing garbage. The method particularly includes the steps of firstly, conducting high-density liquid fermentation on bacillus cereus, lactobacillus acidophilus, bacillus stearothermophilus, pseudomonas pseudolcaligenes and sacharomyces cerevisiae; secondly, preparing super-thick microbial powder with the spore content ranging from 109CFU/g to 1010CFU/g through a spray drying method; thirdly, weighing and taking, in proportion, 30 parts to 100 parts of the bacillus cereus prepared in the first step, 100 parts to 300 parts of the lactobacillus acidophilus prepared in the first step, 100 parts to 150 parts of the bacillus stearothermophilus prepared in the first step, 50 parts to 120 parts of the pseudomonas pseudolcaligenes prepared in the first step and 80 parts to 150 parts of the sacharomyces cerevisiae prepared in the first step, and mixing the mixture even in a stirring machine; fourthly, drying the mixed microbial powder in an air drying cylinder to obtain the microbial agent for processing the garbage. The method for producing the microbial agent is simple, suitable for an existing process and existing fermentation devices, and easy to widely popularize.

The invention discloses a microbial adsorbent for industrial wastewater and a preparation method thereof.Zeolite-loaded pseudomonas pseudoalcaligenes is coated on an inner layer, a hydrogel shell layer is loaded with bacillus subtilis, and the two strains are fixed to different carriers respectively, so that growth of the pseudomonas pseudoalcaligenes and the bacillus subtilis is facilitated; in addition, Fe3O4 nano-particles are fixed on chitosan, then chitosan, polyvinyl alcohol and sodium alginate form a gel structure, the dispersibility of the Fe3O4 nano-particles is better, the Fe3O4 nano-particles are fixed on the chitosan, the Fe3O4 nano-particles are not prone to loss, and the chitosan, polyvinyl alcohol and sodium alginate are mixed to form a gel structure, so that the chitosan, polyvinyl alcohol and sodium alginate are mixed to form the gel structure, and the chitosan, polyvinyl alcohol and sodium alginate are mixed to form the gel structure. The microbial adsorbent can keep stable and lasting wastewater treatment capacity and is good in reusability.