Mixed flora microbial preparation and its application in treating wastewater containing nitrate nitrogen

A technology of microbial preparations and mixed flora, applied in biological water/sewage treatment, microorganism-based methods, microorganisms, etc., can solve the problem of taxonomy without fixed distribution, etc.

Active Publication Date: 2016-03-16
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Studies have shown that denitrifying microorganisms widely exist in soil, stratified rock and water ecological environment, but there is no fixed distribution in taxonomy

Method used

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  • Mixed flora microbial preparation and its application in treating wastewater containing nitrate nitrogen
  • Mixed flora microbial preparation and its application in treating wastewater containing nitrate nitrogen
  • Mixed flora microbial preparation and its application in treating wastewater containing nitrate nitrogen

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1 prepares mixed flora microbial preparation

[0025] The preparation method is as follows:

[0026] Streak on a plate to activate Paenibacillus for 2 days; after activation, pick a single colony and inoculate it in 50ml of seed culture solution, culture it on a shaking table at 30-35°C, 180r / min for 24 hours, and obtain the seed solution; take the seed solution and inoculate it in In the enriched culture solution (inoculum size is 20%, volume percentage), 30~35°C, 180r / min, shaker culture for 24h, to obtain the enriched culture solution; the enriched culture solution was centrifuged at 7000r / min for 20min , remove the supernatant to obtain concentrated thalline cells, dilute to 1.5g wet thalline / L bacterium suspension with sterile water, obtain Paenibacillus bacterium suspension;

[0027] The same method (steps, parameters, and medium are all identical) prepares Pseudomonas stutzeri suspension, Pseudomonas pseudoalcaligenes suspension and Pseudomonas oleovo...

Embodiment 2

[0035] Example 2 Mixed flora microbial preparations treatment of waste water containing nitrate nitrogen experiment

[0036] Experiment is carried out in 250mL conical flask, every bottle 200mL contains nitrate nitrogen waste water (nitrate nitrogen concentration is as high as 136mg / L), totally three bottles, add 1%, 5%, 10% the mixed preparation of embodiment 1 in three bottles respectively Bacterial flora microbial preparation (volume percentage, that is, the amount of mixed flora microbial preparation added to the three bottles is 2ml, 10ml, 20ml respectively), cultured at 30-35°C, and respectively according to the corresponding time (every 8 hours) Take waste water, filter it under a 0.22 μm filter membrane, measure the nitrate nitrogen content in the filtrate, and calculate the denitrification rate.

[0037] The result is as figure 1 As shown in the figure, the Erlenmeyer flask with 1% mixed flora microbial preparation was added, the removal of nitrate nitrogen was at 8h...

Embodiment 3

[0041] The growth curve change of embodiment 3 mixed flora in the treatment of waste water containing nitrate nitrogen experiment

[0042] Experiment is carried out in 250mL conical flask, every bottle 200mL contains nitrate nitrogen waste water (preparation method is the same as embodiment 2), adds the mixed flora microbial preparation prepared in Example 1 (establish three conical flasks, the mixed flora microbial preparation of adding The amount is 1%, 5%, 10% respectively, i.e.: 2ml, 10ml, 20ml), cultured at 30-35°C, and the OD of the wastewater is detected by spectrophotometry at regular intervals 600 (results such as image 3 shown). After the mixed flora was inoculated into the wastewater, as time went on, the bacteria multiplied in large numbers, the denitrification effect of the bacteria was significant, and a large number of bubbles appeared in the wastewater, which gradually became turbid (see figure 2 ). After the flora was inoculated into the wastewater, the g...

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Abstract

The invention discloses a mixed bacterial colony microbial preparation which is a bacterial suspension consisting of paenibacillus, pseudomonas stutzeri, pseudomonas pseudoalcaligenes and pseudomonas oleovoran, wherein the viable count of paenibacillus is more than 1.69*10<10>CFU / Ml, the viable count of pseudomonas stutzeri is more than 2.35*10<10>CFU / Ml, the viable count of pseudomonas pseudoalcaligenes is more than 2.18*10<10>CFU / Ml, and the viable count of pseudomonas oleovoran is more than 2.26*10<10>CFU / Ml. The mixed bacterial colony microbial preparation can be used for treating wastewater containing nitrate nitrogen, and the specific application method comprises the steps of regulating the pH value of the wastewater containing nitrate nitrogen to 7.0-7.2, then inoculating the wastewater containing nitrate nitrogen with the mixed bacterial colony microbial preparation according to the inoculation amount of 1-10%, and performing static culture at 30-35 DEG C. The denitrification effect can be obvious after 4 days culture and complete denitrification can be realized.

Description

technical field [0001] The invention relates to a mixed flora microbial preparation and its application in treating waste water containing nitrate nitrogen, belonging to the technical field of environmental protection. Background technique [0002] At present, with the continuous development of the national economy, the pollution of nitrogen in my country's water bodies is becoming more and more serious, and the problem of eutrophication in water bodies is prominent. The shortage of water resources and the deteriorating water environment have become important factors restricting my country's economic development. Groundwater in most areas of my country suffers from nitrate nitrogen pollution to varying degrees. Excessive nitrate in water is extremely harmful to human health. In the human body, nitrate nitrogen will be reduced to nitrite under the action of microorganisms, causing methemoglobinemia and infantile cyanosis. In addition, nitrate and nitrite will be converted in...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C12R1/38C02F101/38
Inventor 裴海燕邵媛媛胡文容孟盼盼李政
Owner SHANDONG UNIV
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