Waste water treatment agent and application thereof

Abstract

The invention discloses a waste water treatment agent. The waste water treatment agent is prepared by using the following steps: 1, mixed fermenting, culturing and collecting pseudomonas pseudoalcaligenes and saccharomyces cerevisiae thalli; 2, mixing polyvinyl alcohol, calcium alginate, acrylyl and water according to a mass ratio of (6-15):(0.5-1.5):(3-5):100 to obtain a polyvinyl alcohol hydrosol, and adding the collected pseudomonas pseudoalcaligenes and saccharomyces cerevisiae thalli into the polyvinyl alcohol hydrosol to form a mixed solution; 3, adding the mixed solution into a saturated boric acid solution containing N'-methylene bisacrylamide CaSO4 by using a peristaltic pump to form a gel bead, cooling and unfreezing the gel bead, then cooling and unfreezing the gel bead again, placing in phosphate with the mass concentration of 0.2-0.5 g / L to obtain an immobilized cell bead with a spherical appearance and an internal porous mesh structure. The invention also discloses a method for treating waste water by using the treatment agent. Different heavy metals are well adsorbed.

Description

technical field

[0001] The invention relates to the field of sewage treatment, in particular to a waste water treatment agent and its application. Background technique

[0002] At present, there are three main types of heavy metal wastewater treatment methods: chemical reaction method, concentration separation method and biological adsorption method. The first category is the removal of heavy metal ions in wastewater through chemical reactions, including neutralization precipitation, sulfide precipitation, ferrite co-precipitation, chemical reduction, electrochemical reduction, and polymer heavy metal collectors; The second category is the method of concentrating and separating heavy metals in wastewater without changing their chemical form, including adsorbent adsorption, ion exchange and membrane separation; the third category of biosorption is a new development this year. The heavy metal sewage treatment method uses microorganisms and their derivatives to absorb heavy me...

Examples

Embodiment 1

[0022] Embodiment 1 mixed fermentation culture collects Pseudomonas pseudoalcaligenes and Saccharomyces cerevisiae thalline, comprises the steps:

[0023] 1) Cultivation of Saccharomyces cerevisiae seed solution

[0024] Saccharomyces cerevisiae slant strains were inoculated on the primary seed medium for shake flask culture. After cultivating for 24 hours at 30°C with a rotation speed of 260rmp, they were inoculated in the secondary seed medium for fermentation tank cultivation at 30°C with a rotation speed of 260rmp and an aeration volume of 2.5 ~3m 3 After 24 hours of cultivation per hour, the seed liquid of Saccharomyces cerevisiae was obtained, wherein the primary seed medium was: yeast extract 5g / L, peptone 6g / L, glucose 20g / L; the secondary seed medium was: glucose 40g / L , yeast extract 5g / L, peptone 5g / L, (NH 4 ) 2 SO 42g / L, MgSO 4 ·7H 2 O0.25g / L, NaCl0.15g / L, KH 2 PO 4 2.8g / L.

[0025] 2) The cultivation of Pseudomonas alcaligenes seed solution

[0026] The ...

Embodiment 2

[0029] Embodiment 2 A preparation method of immobilized cell spheres, comprising the following steps:

[0030] A) After mixing polyvinyl alcohol, calcium alginate, acryloyl and water according to the mass ratio of 12:1:4:100, polyvinyl alcohol hydrosol is obtained, and the alkaligenic pseudomonas collected in Example 1 Bacteria and Saccharomyces cerevisiae cells were added to polyvinyl alcohol hydrosol to form a mixed solution, wherein the addition amount of Pseudomonas alcaligenes and Saccharomyces cerevisiae cells met the product of adding 5g wet weight per 100mL of polyvinyl alcohol hydrosol. Alkaline Pseudomonas and Saccharomyces cerevisiae mixed bacteria; B) Add the mixed solution to the solution containing 4w / v% N'-methylene bisacrylamide and 1.5w / v% CaSO with a peristaltic pump 4 In a saturated boric acid solution, gel balls are formed, placed at -20°C for 5-10 hours, thawed at room temperature, and then cooled at -10°C for 1-2 hours, after thawing at room temperature, ...

Embodiment 3

[0031] Embodiment 3 A preparation method of immobilized cell spheres, comprising the following steps:

[0032] A) After mixing polyvinyl alcohol, calcium alginate, acryloyl and water according to the mass ratio of 6:0.5:3:100, polyvinyl alcohol hydrosol is obtained, and the alkaligenic pseudomonas collected in Example 1 Bacteria and Saccharomyces cerevisiae cells were added to polyvinyl alcohol hydrosol to form a mixed solution, and the addition amount of Pseudomonas alcaligenes and Saccharomyces cerevisiae cells was controlled to meet the production of 4g wet weight per 100mL of polyvinyl alcohol hydrosol. Alkali Pseudomonas and Saccharomyces cerevisiae mixed bacteria; then the mixed solution was added to the solution containing 3w / v% N'-methylene bisacrylamide and 1w / v% CaSO with a peristaltic pump 4 In a saturated boric acid solution, gel balls are formed, placed at -20°C for 5-10 hours, thawed at room temperature, and then cooled at -10°C for 1-2 hours, after thawing at ro...

technical field

[0001] The invention relates to the field of sewage treatment, in particular to a waste water treatment agent and its application. Background technique

[0002] At present, there are three main types of heavy metal wastewater treatment methods: chemical reaction method, concentration separation method and biological adsorption method. The first category is the removal of heavy metal ions in wastewater through chemical reactions, including neutralization precipitation, sulfide precipitation, ferrite co-precipitation, chemical reduction, electrochemical reduction, and polymer heavy metal collectors; The second category is the method of concentrating and separating heavy metals in wastewater without changing their chemical form, including adsorbent adsorption, ion exchange and membrane separation; the third category of biosorption is a new development this year. The heavy metal sewage treatment method uses microorganisms and their derivatives to absorb heavy me...

Examples

Embodiment 1

[0022] Embodiment 1 mixed fermentation culture collects Pseudomonas pseudoalcaligenes and Saccharomyces cerevisiae thalline, comprises the steps:

[0023] 1) Cultivation of Saccharomyces cerevisiae seed solution

[0024] Saccharomyces cerevisiae slant strains were inoculated on the primary seed medium for shake flask culture. After cultivating for 24 hours at 30°C with a rotation speed of 260rmp, they were inoculated in the secondary seed medium for fermentation tank cultivation at 30°C with a rotation speed of 260rmp and an aeration volume of 2.5 ~3m 3 After 24 hours of cultivation per hour, the seed liquid of Saccharomyces cerevisiae was obtained, wherein the primary seed medium was: yeast extract 5g / L, peptone 6g / L, glucose 20g / L; the secondary seed medium was: glucose 40g / L , yeast extract 5g / L, peptone 5g / L, (NH 4 ) 2 SO 42g / L, MgSO 4 ·7H 2 O0.25g / L, NaCl0.15g / L, KH 2 PO 4 2.8g / L.

[0025] 2) The cultivation of Pseudomonas alcaligenes seed solution

[0026] The ...

Embodiment 2

[0029] Embodiment 2 A preparation method of immobilized cell spheres, comprising the following steps:

[0030] A) After mixing polyvinyl alcohol, calcium alginate, acryloyl and water according to the mass ratio of 12:1:4:100, polyvinyl alcohol hydrosol is obtained, and the alkaligenic pseudomonas collected in Example 1 Bacteria and Saccharomyces cerevisiae cells were added to polyvinyl alcohol hydrosol to form a mixed solution, wherein the addition amount of Pseudomonas alcaligenes and Saccharomyces cerevisiae cells met the product of adding 5g wet weight per 100mL of polyvinyl alcohol hydrosol. Alkaline Pseudomonas and Saccharomyces cerevisiae mixed bacteria; B) Add the mixed solution to the solution containing 4w / v% N'-methylene bisacrylamide and 1.5w / v% CaSO with a peristaltic pump 4 In a saturated boric acid solution, gel balls are formed, placed at -20°C for 5-10 hours, thawed at room temperature, and then cooled at -10°C for 1-2 hours, after thawing at room temperature, ...

Embodiment 3

[0031] Embodiment 3 A preparation method of immobilized cell spheres, comprising the following steps:

[0032] A) After mixing polyvinyl alcohol, calcium alginate, acryloyl and water according to the mass ratio of 6:0.5:3:100, polyvinyl alcohol hydrosol is obtained, and the alkaligenic pseudomonas collected in Example 1 Bacteria and Saccharomyces cerevisiae cells were added to polyvinyl alcohol hydrosol to form a mixed solution, and the addition amount of Pseudomonas alcaligenes and Saccharomyces cerevisiae cells was controlled to meet the production of 4g wet weight per 100mL of polyvinyl alcohol hydrosol. Alkali Pseudomonas and Saccharomyces cerevisiae mixed bacteria; then the mixed solution was added to the solution containing 3w / v% N'-methylene bisacrylamide and 1w / v% CaSO with a peristaltic pump 4 In a saturated boric acid solution, gel balls are formed, placed at -20°C for 5-10 hours, thawed at room temperature, and then cooled at -10°C for 1-2 hours, after thawing at ro...

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