The invention provides a temperature-controlled magnetic tweezer device comprising a
microscopic imaging apparatus, a magnetic tweezer apparatus, a
sample pool, a central monitor, a sample bench and a
temperature control apparatus, wherein the magnetic tweezer apparatus comprises a magnetic tweezer and a magnetic tweezer controller, and the magnetic tweezer is horizontally arranged at a side surface of the
sample pool and controlled by the magnetic tweezer controller. One end of a biological single molecule is connected to a sidewall away from the magnetic tweezer in the
sample pool, and the other end is connected with a paramagnetic magnetic ball which passes through the magnetic tweezer controller. The sample bench is provided with a sample
pool groove for accommodating the sample
pool, wherein the sample
pool is arranged in the sample pool groove, and the sample bench is prepared by heat-
conductive materials. According to the invention, a
temperature control function of the sample pool is improved, the controller and
semiconductor refrigeration sheets, as well as the heat-conductive sample bench and heat dissipation units can be used to heat or cool, and finally a target temperature in the sample pool can be achieved. The sample pool is a nearly integral structure, thereby being simple in manufacturing steps, convenient to clean, capable of repeatable use, material-saving, and good in sealing.