Temperature-controlled magnetic tweezer device

Abstract

The invention provides a temperature-controlled magnetic tweezer device comprising a microscopic imaging apparatus, a magnetic tweezer apparatus, a sample pool, a central monitor, a sample bench and a temperature control apparatus, wherein the magnetic tweezer apparatus comprises a magnetic tweezer and a magnetic tweezer controller, and the magnetic tweezer is horizontally arranged at a side surface of the sample pool and controlled by the magnetic tweezer controller. One end of a biological single molecule is connected to a sidewall away from the magnetic tweezer in the sample pool, and the other end is connected with a paramagnetic magnetic ball which passes through the magnetic tweezer controller. The sample bench is provided with a sample pool groove for accommodating the sample pool, wherein the sample pool is arranged in the sample pool groove, and the sample bench is prepared by heat-conductive materials. According to the invention, a temperature control function of the sample pool is improved, the controller and semiconductor refrigeration sheets, as well as the heat-conductive sample bench and heat dissipation units can be used to heat or cool, and finally a target temperature in the sample pool can be achieved. The sample pool is a nearly integral structure, thereby being simple in manufacturing steps, convenient to clean, capable of repeatable use, material-saving, and good in sealing.

Description

technical field [0001] The invention belongs to the field of single molecule manipulation and detection, in particular to a temperature-controllable magnetic tweezers device. Background technique [0002] The rise of molecular biology has made life science gradually get rid of the descriptive research mode, and go deep into the molecular level to reveal the essence of life phenomena. Despite the complexity of living systems, people are accustomed to thinking about problems and building models based on the interactions between single molecules. Correspondingly, people have been looking forward to the direct study of fundamental life processes at the single-molecule level. Single-molecule research technologies developed since the 1990s, such as optical tweezers, magnetic tweezers, glass microtubes, and molecular combs, together with single-molecule fluorescence technology, enable people to directly manipulate and detect the movement and changes of single molecules. Or change...

AI Technical Summary

Problems solved by technology

[0007] However, at present, the internal temperature of the sample cell of the existing magnetic tweezers can only be room temperature, and cannot be manually adjusted to keep the temperature constant at a temperature higher than room temperature or lower than room temperature; in addition, the design of the sample cell of the magnetic tweezers is relatively elementary, and it is very dependent on manual production Level, if the production level is not up to standard, the sample pool will leak, and the imaging surface will be blurred by the sealant and cannot be clearly imaged; and because the sample pool is nearly sealed, it is difficult to clean and reuse after use

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