Denitrifying phosphate-accumulating organism with nitrogen and phosphorus removal functions and applications thereof

A technology for denitrifying phosphorus-accumulating bacteria and denitrifying and dephosphorizing, applied in bacteria, chemical instruments and methods, microorganism-based methods, etc. Active, stable effect, simple process effect

Active Publication Date: 2013-05-22
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the research on this type of bacteria and their characteristics is still in its infancy, there is no systematic identification method, and there is no convenient and quick way of cultivating and domesticating

Method used

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  • Denitrifying phosphate-accumulating organism with nitrogen and phosphorus removal functions and applications thereof
  • Denitrifying phosphate-accumulating organism with nitrogen and phosphorus removal functions and applications thereof
  • Denitrifying phosphate-accumulating organism with nitrogen and phosphorus removal functions and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The isolation and identification of embodiment 1 bacterial strain of the present invention:

[0033] (1) Medium:

[0034] A. Strain isolation, purification, preservation medium ( / L):

[0035] CH 3 COONa, 2g; Peptone, 15g; Yeast extract, 3g; Glucose, 1g; NaCl, 6g; Agar, 12g; KNO 3, 1.5g; the pH is controlled at 7.0-7.2.

[0036] B. Strain screening, denitrifying medium (DM: Denitrifying Medium) ( / L):

[0037] CH 3 COONa, 2g; KH 2 PO 4 , 0.4g; MgSO 4 ·7H 2 O, 0.6g; CaCl 2 2H 2 O, 0.07g; KNO 3 , 1g; Tris buffer 12mL; trace elements 2mL; pH controlled at 7.0-7.2.

[0038] C. LB medium ( / L):

[0039] Peptone, 10g; yeast extract, 5g; sodium chloride, 10g; pH is controlled at 7.5.

[0040] (2) Isolation and purification of Pseudomonas CL-1 strain:

[0041] Separation and purification were carried out by dilution coating method and plate streaking method. First, use a sterile pipette to take 10 mL of the sludge mixture solution at the end of SBR hypoxia, put it ...

Embodiment 2

[0060] The cultivation of embodiment 2 bacterial strains of the present invention

[0061] (1) Medium used

[0062] A. Strain preservation medium ( / L): peptone, 5g; yeast extract, 3g; glucose, 1g; NaCl, 6g; agar, 12g; KNO 3 , 1.5g; the pH is controlled at 7.0-7.2.

[0063] B. Strain denitrification medium (DM: Denitrifying Medium) ( / L): CH 3 COONa, 2g; KH 2 PO 4 , 0.4g; MgSO 4 ·7H 2 O, 0.6g; CaCl 2 2H 2 O, 0.07g; KNO 3 , 1g; Tris buffer 12mL; trace elements 2mL; pH controlled at 7.0-7.2.

[0064] The above medium was sterilized at 121°C for 20 minutes before use.

[0065] (2) Culture conditions

[0066] Use an inoculation loop to scrape 1 ring of bacterial lawn from the Pseudomonas CL-1 preserved on the slant of the preservation medium, inoculate it into a 250mL Erlenmeyer flask containing 100mL of sterilized LB medium, and cultivate it with constant temperature and shaking at 30°C After 12 hours, the seed liquid can be obtained. During the experiment, the seed li...

Embodiment 3

[0067] The optimum denitrification and dephosphorization conditions of the bacterial strain of the present invention in embodiment 3

[0068] Put 100mL of sterilized denitrification medium into a 250mL Erlenmeyer flask, insert 10% of the inoculum into the seed bacterial solution, and let it stand for cultivation. The optimal denitrification temperature is between 25-30°C, the optimum temperature is 25°C, and the optimum pH value is 7-8. The growth cycle of the bacteria is long, entering the logarithmic growth phase after 48h, entering the stable phase after 96h, basically in the stable phase from 96 to 120h, and entering the decline phase after 120h. The nitrogen and phosphorus removal rates of the strain were 56.93% and 43.56% in the first 24 hours, and the nitrogen and phosphorus removal rates were 75.6% and 57.5% during the whole experiment.

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Abstract

The invention relates to a denitrifying phosphate-accumulating organism with nitrogen and phosphorus removal functions and applications thereof. The strain is named as pseudomonas pseudoalcaligenes CL-1, belongs to pseudomonas pseudoalcaligenes, and is preserved in China Center for Type Culture Collection on June 13, 2012, and the preservation numbers is CCTCC NO: M2012225. The strain is gram-positive and belongs to brevibacterium, a bacterial colony is rounded, milk white, convex and glossy, and edges are smooth and transparent. The growth cycle of the organism is long, the nitrogen and phosphorus removal rates in the first 24h are respectively 56.93% and 43.56%, and the nitrogen and phosphorus removal rates in the whole growth cycle are respectively 75.6% and 57.5%. The strain has a complete denitrifying capacity, and can directly reduce inorganic nitrogen such as nitrate nitrogen and nitrite nitrogen and the like to harmless nitrogen gas discharged from water bodies. When the denitrifying phosphate-accumulating organism is used for treating nitrogen and phosphorus containing wastewater, the process is simple, the treatment effect is efficient and stable, and the operating cost is saved, meanwhile, no greenhouse gas such as NO and N2O and the like is produced, therefore, no air pollution is caused.

Description

technical field [0001] The invention relates to the cultivation and application of a facultative anaerobic denitrifying phosphorus-accumulating bacterium with a complete denitrifying enzyme system and belongs to the field of environmental microorganisms. Background technique [0002] With the rapid development of my country's economy, water pollution has become a major concern of all sectors of society. At present, the main pollutants in the domestic water environment include organic matter and inorganic pollutants such as total nitrogen and total phosphorus. Among them, total nitrogen and total phosphorus are the most harmful to water bodies. A large amount of nitrogen and phosphorus entering the water will cause a sharp deterioration of the water environment. The harm lies in the eutrophication of water bodies. In the past ten years, the proportion of eutrophic water bodies in my country has increased from 50% to 55%, while the proportion of oligotrophic water bodies has de...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/38
Inventor 裴海燕孟盼盼胡文容邵媛媛李政
Owner SHANDONG UNIV
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