A Strain of Pseudomonas-like Alcaligenes nyj3.6 and Its Application

A technology of NYJ3.6 and Pseudomonas, applied in the field of microorganisms, can solve the problems of inconvenient recovery of immobilized carriers, and achieve the effect of promoting oil degradation and solving oil pollution

Active Publication Date: 2022-03-04
HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although biochar has the benefits of increasing the carbon content of the soil environment, the inconvenience of recovering the immobilized carrier after surface water oil pollution adsorption method is also a technical problem at present.

Method used

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  • A Strain of Pseudomonas-like Alcaligenes nyj3.6 and Its Application
  • A Strain of Pseudomonas-like Alcaligenes nyj3.6 and Its Application
  • A Strain of Pseudomonas-like Alcaligenes nyj3.6 and Its Application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1: Illustrate the separation, purification and screening of strains:

[0031] From the petroleum-contaminated soil in Shengli Oilfield, Bohai Sea, Liaoning, weighed three different degrees of petroleum-contaminated soil, and inoculated them into 90 mL of sterilized inorganic salt medium by aseptic operation, at 37 °C and 150 r / min, Shake the culture for 7 days, take 10 mL of the enriched liquid in a new crude oil medium, and culture continuously for 4 to 5 times. The crude oil medium configuration method is as follows: take 0.2 g of crude oil and sterilize it in 100 mL of inorganic salt medium. The specific parameters of pH, salinity and oil content of the medium during the acclimation process are as shown in Table 1:

[0032] Table 1 Parameter settings in the domestication process

[0033]

[0034] After the acclimatization is completed, take an appropriate amount of culture solution and spread it on the LB solid plate. After about 24 hours, colonies gro...

Embodiment 2

[0035] Embodiment 2: the degradation rate of different isolated and purified bacterial strains to petroleum:

[0036] Pre-freeze the plate of the bacterial suspension in a -16°C refrigerator for 4 hours, then transfer it to a vacuum freeze dryer at a vacuum degree of 54-60Pa, and dry at -45°C for 48 hours to obtain dry bacteria powder. Add 0.2 g of crude oil to 100 mL of inorganic salt medium for high-temperature sterilization to prepare crude oil medium, and add the prepared dried bacteria powder after cooling. Add the dried bacteria powder to the sterilized crude oil medium at an inoculum amount of 0.2g dry powder per 100mL medium, make three groups for each strain, and have three bottles of crude oil medium without bacteria as the blank control, and keep the temperature on the shaker Shake culture for 7 days to observe the oil degradation effect of the strain and measure the degradation rate.

[0037] Add 10 to 20 mL of petroleum ether with a boiling range of 60 to 90°C to...

Embodiment 3

[0045] Embodiment 3: Description of the morphology and biochemical characteristics of bacterial strains:

[0046] Observe the morphological characteristics such as the edge shape, size, color, uplift, transparency and other morphological characteristics of the colonies on the medium plate of the 4 high-efficiency petroleum-degrading bacteria NYJ1.13, NYJ3.4, NYJ3.6, and NYJ3.7 obtained through isolation and screening. Refer to Berger Bacterial smears were prepared according to the Handbook of Bacteria Classification and Identification, Gram staining, and individual shape and size were observed under a microscope. The colony morphology of the four strains is shown in Table 3. Using traditional bacterial identification methods and bacterial biochemical identification tubes (Hangzhou Microbiology Co., Ltd.) to systematically detect the physical and chemical characteristics of petroleum-degrading bacteria, the biochemical identification tubes were used to identify petroleum-degradi...

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Abstract

The invention relates to a strain of Pseudomonas pseudoalcaligenes (Pseudomonas pseudoalcaligenes) NYJ3.6. The invention also relates to the application of the above bacteria in petroleum degradation and a floating carbon immobilized bacteria agent containing the above NYJ3.6, and provides the preparation method and application of the bacteria agent. The Pseudomonas pseudoalcaligenes NYJ3.6 provided by the present invention has the effect of promoting petroleum degradation, and can be used to solve the problem of petroleum pollution; secondly, the floating carbon immobilized bacteria agent containing the above-mentioned Pseudomonas alcaligenes NYJ3.6, Using 100 mesh above 800°C reed rods as the biochar immobilization carrier, the biochar can adsorb oil more efficiently before its own precipitation. After absorbing oil, its buoyancy can be improved while reducing the surface viscosity of oil, which can Promote oil degradation more effectively.

Description

technical field [0001] The invention belongs to the field of microorganisms, and relates to a strain of Pseudomonas NYJ3.6 and its application, in particular to a strain of Pseudomonas NYJ3.6 capable of promoting oil degradation in saline-alkali land and its application. Background technique [0002] Petroleum is an important raw material for many products in life. While this non-renewable resource brings convenience to human beings, it also threatens the ecological environment and human health due to many improper operations or accidents. The main components of petroleum are mixtures of alkanes, cycloalkanes, and aromatic hydrocarbons, among which there are many components that are harmful to humans. After polluting the environment, they will also enter the biological body through material circulation. As an oil city, Daqing is characterized by its large wetland area, sufficient surface water, high salinity and alkali content in the water, and an annual average temperature ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N11/14C02F3/34C12R1/38C02F101/32
CPCC12N1/20C12N11/14C02F3/344C02F2101/32C12R2001/38C12N1/205
Inventor 曹迪董程赵子奇黄玲巍单荣
Owner HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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