Kitchen garbage biological fermentation microbial inoculum and application thereof

A technology for biological fermentation bacteria and kitchen waste, which is applied in the directions of microorganisms, microorganism-based methods, biochemical equipment and methods, etc. Low, short fermentation time, good reduction effect

Inactive Publication Date: 2019-10-18
NANKAI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, a variety of food waste treatment technologies have been developed for food waste. However, the fermentation bacteria used in the existing technology are mostly EM complex bacteri

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A kind of food waste biological fermentation bacterial agent and application thereof, the preparation method of the food waste biological fermentation bacterial agent is as follows:

[0032] 1) Cultivate Bacillus subtilis, Bacillus licheniformis, Pseudomonas pseudoalcaligenes, Rhodopseudomonas palustris, and Actinomyces frankii in liquid LB medium at 30°C for 20 hours; Yeasts were cultured in liquid PDA medium at 28°C for 72 hours;

[0033] 2) Centrifuge the above bacterial liquid at 10,000rpm for 30 minutes, discard the supernatant, and take the sediments according to the following conditions: Bacillus subtilis: Bacillus licheniformis: Pseudomonas pseudoalcaligenes: Rhodopseudomonas palustris: Frank's Fibromycetes: Debaria hansenii is mixed with wheat bran at 18:5:20:15:20:21, and freeze-dried in a freeze dryer to obtain a biological fermentation agent for food waste.

[0034] Mix and ferment the above-mentioned fermented bacteria agent with the food waste composed of...

Embodiment 2

[0043] A kind of food waste biological fermentation bacterial agent and application thereof, the preparation method of the food waste biological fermentation bacterial agent is as follows:

[0044] 1) Cultivate Bacillus subtilis, Cellulomonas chrysogenum, Pseudomonas pseudoalcaligenes, and Rhodopseudomonas palustris in liquid LB medium at 32°C for 22 hours; Debaryomyces hansenii, Candida tropicalis was cultured in liquid PDA medium at 33°C for 56 hours;

[0045] 2) Centrifuge the above bacterial solution at 10,000rpm for 25 minutes, discard the supernatant, and take the sediments according to the following conditions: Debaryomyces hansenii: Candida tropicalis is mixed with straw at 25:15:14:10:26:10, and freeze-dried in a freeze dryer to obtain a biological fermentation agent for food waste.

[0046] Mix and ferment the above-mentioned fermented bacteria agent and the food waste composed of leftovers from the canteen, and the fermentation steps are as follows:

[0047] 1) Ad...

Embodiment 3

[0055]A kind of food waste biological fermentation bacterial agent and application thereof, the preparation method of the food waste biological fermentation bacterial agent is as follows:

[0056] 1) Cultivate Bacillus subtilis, Pseudomonas pseudoalcaligenes, Rhodopseudomonas palustris, and Actinomyces frankii in liquid LB medium at 35°C for 18 hours; Candida was cultured in liquid PDA medium at 34°C for 48 hours;

[0057] 2) Centrifuge the above bacterial liquid at 9000rpm for 28 minutes, discard the supernatant, and take the sediments according to the following conditions: Bacillus subtilis: Sunderbarreya: Candida tropicalis at 35:17:10:20:10:8 is mixed with sawdust and freeze-dried in a freeze dryer to obtain a biological fermentation agent for food waste.

[0058] Mix and ferment the above-mentioned fermented bacteria agent with the kitchen waste composed of vegetable leaves and fruit peels and stones in the kitchen, and the fermentation steps are as follows:

[0059] 1)...

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PUM

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Abstract

The invention relates to kitchen garbage biological fermentation microbial inoculum. The kitchen garbage biological fermentation microbial inoculum consists of four or more of bacillus subtilis, bacillus licheniformis, cellulomonas flavigena, pseudomonas pesodoalcaligenes, rhodop seudanonas palustris, frankiaceae actinomycesbovis, debaryomyces hansenii, and candida tropicalis and is loaded to a carrier consisting of one or two of wheat bran, saw dust or straw, and is subjected to freeze drying to obtain the microbial inoculum; and the microbial inoculum is mixed with kitchen garbage, and a fermentation reaction is performed under 50-65 DEG C, so that the kitchen garbage can be reduced by 70% within 6-10 hours, and the content of generated organic matter exceeds 90%. The kitchen garbage biological fermentation microbial inoculum has the advantages that the technical method can efficiently reduce kitchen garbage, the treated substances can be used for soil modification, and the kitchen garbage biological fermentation microbial inoculum has remarkable economic, social and environmental benefits.

Description

technical field [0001] The invention relates to a microbial bacterial agent for treating kitchen garbage, in particular to a biological fermentation bacterial agent for kitchen garbage and its application. Background technique [0002] Food waste is the most important type of food waste, including food processing waste and food residues generated by families, schools, canteens and catering industries. Food waste is mainly composed of grease, water, fruit peels, vegetables, rice noodles, fish, meat, bones, waste tableware, plastics, paper towels and other substances. The output of food waste in my country is huge, with an annual output of more than 100 million tons, and it is showing a rapid upward trend. Because the water content in food waste is as high as 90%, and the salt content is relatively high. The current main treatment methods for food waste include incineration and landfill. Due to the high moisture content and low calorific value, the incineration process cons...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16C05F9/04C12R1/125C12R1/10C12R1/38C12R1/01C12R1/04C12R1/74C12R1/645
CPCC12N1/20C12N1/16C05F9/04Y02A40/20
Inventor 李维尊季鹏孙希财鞠美庭
Owner NANKAI UNIV
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