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Complex microbial agent for stabilizing treatment of stock garbage in cities and towns as well as preparation method and application of complex microbial agent

A technology of stabilization treatment and compound bacterial agent, which is applied in the fields of biotechnology and environmental protection, can solve the problems of large energy consumption, difficulty in achieving stable and harmless treatment, and increase in operating costs of domestic waste landfills, and achieve the reduction of malodorous gases , to achieve the effect of harmlessness and reduction of inhibition

Active Publication Date: 2019-06-07
BEIJING GUOHUAN TSINGHUA ENVIRONMENT ENG DESIGN & RES INST CO LTD BEIJING CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the existing technologies including the above-mentioned reports need to blow high-pressure gas into the interior of the stored garbage, and at the same time need to pump out more gas to maintain a negative pressure inside to maintain an aerobic treatment environment. This process requires a large amount of energy consumption, making The operating period cost of domestic waste landfill is greatly increased
At the same time, it is difficult to achieve stable and harmless treatment of toxic and harmful substances such as high-concentration leachate and some heavy metals in landfills

Method used

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  • Complex microbial agent for stabilizing treatment of stock garbage in cities and towns as well as preparation method and application of complex microbial agent
  • Complex microbial agent for stabilizing treatment of stock garbage in cities and towns as well as preparation method and application of complex microbial agent
  • Complex microbial agent for stabilizing treatment of stock garbage in cities and towns as well as preparation method and application of complex microbial agent

Examples

Experimental program
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Effect test

preparation example Construction

[0052] 4. Preparation of compound bacterial agent

[0053] Mix the fermentation cultures of the components of the composite microbial agent obtained above, and adjust the mass percentage of the added fermentation culture to obtain a composite bacterial agent containing a specific effective number of viable bacteria.

[0054]Among the present invention, the effective viable count of each bacterial strain accounts for the ratio of the total effective viable count of the compound bacterial agent as follows:

[0055]

[0056] The formula of above-mentioned each culture medium is as follows:

[0057] Medium ①: 0.50% yeast extract, 1% peptone, 0.50% beef extract, 0.50% sodium chloride, 0.5% glucose, 2% agar, pH 7.0-7.2.

[0058] ② Medium: 0.5% corn flour, 0.5% soybean meal, 2% maltose, 0.5% glucose, 1.5% corn steep liquor, 2% ammonium sulfate, 0.03% magnesium sulfate, 0.04% potassium dihydrogen phosphate, 2% agar, pH 6.5-7.2.

[0059] ③ Medium: 2% corn flour, 1% sucrose, 1% ma...

Embodiment 1

[0070] The present embodiment provides a kind of compound bacterial agent for stabilizing urban stock garbage, and the total effective number of viable bacteria of the composite bacterial agent is 10 10 cfu / mL, the effective number of viable bacteria of each strain accounted for the percentage of the total effective number of viable bacteria in the compound bacterial agent is as follows:

[0071]

Embodiment 2

[0073] The present embodiment provides a kind of urban stock garbage stabilization composite bacterial agent, the total effective number of viable bacteria of the composite bacterial agent is 2 * 10 10 cfu / mL, the effective number of viable bacteria of each strain accounted for the percentage of the total effective number of viable bacteria in the compound bacterial agent is as follows:

[0074]

[0075]

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Abstract

The invention provides a complex microbial agent for stabilizing treatment of stock garbage in cities and towns as well as a preparation method and an application of the complex microbial agent. The complex microbial agent contains sporotrichum thermophile, pseudomonas alcaligenes, halobacillus sp, bacillus subtilis, pseudomonas putida, candida utilis, saccharomyces cerevisiae, phanerochaete chrysosporium and lactobacillus plantarum. Growth metabolism, degradability and activity of all microbial strains in the complex microbial agent can effectively complement and cooperate with one another, the microbial strains can grow and propagate quickly in a landfill system to promote the temperature of a garbage treatment environment to increase quickly, meanwhile, organic matter, BDM and toxic andharmful substances in a landfill can be quickly and efficiently degraded, the stabilizing time of the garbage landfill is greatly shortened, stable harmless treatment of landfill is promoted, and thecomplex microbial agent can be applied to aerobic stabilizing treatment of garbage in cities and towns in practice.

Description

technical field [0001] The invention relates to the fields of biotechnology and environmental protection, in particular to a composite bacterial agent used for stabilizing treatment of urban stock garbage and its preparation method and application. Background technique [0002] The stock of garbage in cities and towns contains a large amount of organic matter, which is easy to rot and breed mosquitoes and flies, and naturally decomposes slowly after being buried in the ground. For a long time, the traditional treatment methods for the stock of waste are mainly the sealing of the seepage wall and the collection and disposal of gas and leachate. These treatment methods may take 10 to 20 years for the waste to reach a stable period, resulting in a large amount of land that cannot be developed and utilized for a long time , and the operating cost will continue to increase over time, resulting in unnecessary waste of resources, and easily lead to secondary pollution caused by lea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16C12N1/14C05F9/04C12R1/38C12R1/07C12R1/645
CPCY02A40/20
Inventor 王迪宋磊于诗卓吕秀芬刘奇林陈蓓张梅马卓然李丽华郭中杰
Owner BEIJING GUOHUAN TSINGHUA ENVIRONMENT ENG DESIGN & RES INST CO LTD BEIJING CHINA
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