Composite probiotic agent for improving egg quality of laying hens, preparation method and application

A compound probiotic and egg quality technology, applied in biochemical equipment and methods, bacteria used in food preparation, and microbial-based methods, to achieve the effects of improving egg production quality, intestinal function, and nutritional value

Active Publication Date: 2020-10-16
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, some scholars in China have added Bacillus Velez to animal feed, and experiments have proved that Bacillus Velez can significantly reduce diarrhea in animals, but the effect of Bacillus Velez on the egg production quality of laying hens, No one has reported yet

Method used

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  • Composite probiotic agent for improving egg quality of laying hens, preparation method and application
  • Composite probiotic agent for improving egg quality of laying hens, preparation method and application

Examples

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Effect test

Embodiment 1

[0019] This embodiment provides a compound probiotic agent for improving the quality of laying eggs, including compound probiotic bacteria and a carrier, and the preparation method of the compound probiotic agent comprises the following steps:

[0020] (1) After activating Bacillus Velez, plant lactic acid bacteria, Bacteroides faecalis and Pseudomonas alcaligenes respectively, they were inoculated in LB liquid medium for expanded culture;

[0021] (2) Bacillus Veles and Lactobacillus plantarum were expanded and cultured to a colony concentration of 4.0×10 10 CFU / mL, Bacteroides faecalis and Pseudomonas alcaligenes were expanded to a colony concentration of 1.0×10 8 CFU / mL, and then mix the four bacterial solutions in equal volumes to obtain a mixed bacterial solution of compound probiotics;

[0022] (3) Prepare the carrier: mix the rice bran powder and starch according to the mass ratio of 1:8;

[0023] (4) Add 1kg of carrier to every 100ml of compound probiotics, and mix w...

Embodiment 2

[0025] The present embodiment provides a kind of composite probiotic agent that improves the quality of laying eggs, including composite probiotic bacteria and carrier, and the preparation method of this composite probiotic agent comprises the following steps:

[0026] (1) Inoculate Bacillus Velez, Lactobacillus plantarum, Bacteroides faecalis and Pseudomonas alcaligenes in LB liquid medium for expanded culture respectively;

[0027] (2) Bacillus Veles and Lactobacillus plantarum were expanded and cultured to a colony concentration of 4.5×10 10 CFU / mL, Bacteroides faecalis and Pseudomonas alcaligenes were expanded to a colony concentration of 1.5×10 8 CFU / mL, and then mix the four bacterial solutions in equal volumes to obtain a mixed bacterial solution of compound probiotics;

[0028] (3) Prepare the carrier: mix the rice bran powder and starch according to the mass ratio of 1:10;

[0029] (4) Add 1.5kg of carrier to every 100ml of compound probiotics, and mix well to obtai...

Embodiment 3

[0031] The present embodiment provides a kind of composite probiotic agent that improves the quality of laying eggs, including composite probiotic bacteria and carrier, and the preparation method of this composite probiotic agent comprises the following steps:

[0032] (1) Inoculate Bacillus Velez, Lactobacillus plantarum, Bacteroides faecalis and Pseudomonas alcaligenes in LB liquid medium for expanded culture respectively;

[0033] (2) Expand the cultivation of Bacillus Velez and Lactobacillus plantarum to a colony concentration of 4.2×10 10 CFU / mL, Bacteroides faecalis and Pseudomonas alcaligenes were expanded to a colony concentration of 1.2×10 8 CFU / mL, and then mix the four bacterial solutions in equal volumes to obtain a mixed bacterial solution of compound probiotics;

[0034] (3) Prepare the carrier: mix the rice bran powder and starch according to the mass ratio of 1:9;

[0035] (4) Add 1.2kg of carrier to every 100ml of compound probiotics, and mix well to obtain ...

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Abstract

The invention discloses a composite probiotic agent for improving egg quality of laying hens, a preparation method and application. The composite probiotic agent comprises composite probiotics and a carrier, wherein the compound probiotics comprise 4.0-4.5*10<10>CFU / mL of bacillus velezensis, 4.0-5.0*10<10>CFU / mL of lactobacillus plantarum, 1.0-1.5*10<8>CFU / mL of bacteroides faecalis and 1.0-1.5*10<8>CFU / mL of pseudomonas alcaligenes; and the carrier comprises rice chaff powder and starch. Compared with the prior art, the compound probiotic agent disclosed by the invention not only can improvethe intestinal function of the laying hens, but also can improve the color and taste of eggs and improve the egg quality of the laying hens, especially can effectively increase the proportion of unsaturated fatty acid omega-3 / omega-6 in the eggs and improve the nutritional value of the eggs; and therefore, the composite probiotic agent is suitable for being used as feed additives for large-scalebreeding.

Description

technical field [0001] The invention relates to the technical field of feed additives for laying hens, in particular to a compound probiotic agent for improving the quality of laying eggs, a preparation method and an application. Background technique [0002] In the large-scale breeding of laying hens, due to the influence of various factors, the quality of eggs is reduced. Therefore, how to improve the egg quality of farms has always been a prominent problem that plagues farms. Especially in the late laying period of laying hens, the eggshell quality of eggs is significantly reduced, mainly manifested in problems such as broken shell eggs, soft shell eggs, sandy shell eggs, blood spot eggs and meat spot eggs. Many factors affect the quality of these eggs, including genetic factors, environmental factors, disease, nutrition, and stress. Hough Unit is an important basis for evaluating the quality and freshness of laying hens. The higher the consistency of egg white and the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/18A23K10/37A23K20/163A23K50/75C12N1/20C12R1/07C12R1/25C12R1/01C12R1/38
CPCA23K10/18A23K10/37A23K20/163A23K50/75C12N1/20A23V2400/169Y02P60/87
Inventor 王在贵刘加乐詹凯孙玲红马瑞钰
Owner ANHUI AGRICULTURAL UNIVERSITY
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