Biological organic fertilizer and preparation method thereof
A bio-organic fertilizer and raw material technology, applied in the agricultural field, can solve the problems of high processing costs and large investment, and achieve the effects of protecting soil, reducing the use of chemical fertilizers and pesticides, and improving crop quality
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[0027] The preparation method of described lactobacillus plantarum additive is specifically:
[0028] In the first step, after activating Lactobacillus plantarum at 32-36°C for 24 hours, inoculate it in the enriched culture solution, and cultivate it for 24 hours at a temperature of 32-36°C and a rotation speed of 220-250rpm;
[0029] The second step is to inoculate the enriched strains in the aerobic fermentation culture solution according to the volume fraction of 2-5%, and carry out aerobic fermentation culture. The culture conditions are: temperature 32-36 ° C, rotation speed 170-200 rpm, ventilation 0.8~1.0L∕L·min, the incoming air passes through a 0.45μm filter membrane to filter and sterilize, the aerobic fermentation culture time is 20h~24h, and finally air-dried or dried at 30°C to obtain solid bacterial agents, solid bacterial agents The bacteria content in the 10-11 cfu / g.
[0030] The composition of the enriched culture solution is 5.0-6.0 g of sodium chloride, 5...
Embodiment 1
[0062] The preparation of embodiment 1 lactobacillus plantarum additive
[0063] Lactobacillus plantarum (provided by Shanghai Enzyme Biological Consumables Co., Ltd.) was activated at 33°C for 24 hours, and then inoculated in the enriched culture solution, the composition of which was 5.0 g of sodium chloride, 5.0 g of peptone, beef powder 2.5g, distilled water 1000mL, pH 7.0, cultured at a temperature of 33°C and a rotational speed of 250rpm for 24 hours, and then inoculated the enriched strains in the aerobic fermentation medium at a volume fraction of 3% for aerobic fermentation. The composition of the fermentation culture medium is: glucose 50g, magnesium sulfate 20g, potassium dihydrogen phosphate 20g, dipotassium hydrogen phosphate 30g, distilled water 1000mL, adjust the pH value to 7.0, and the culture conditions are: temperature 33°C, rotation speed 200rpm, ventilation rate 1.0L ∕L min, the incoming air is filtered through a 0.45μm filter membrane, the aerobic ferment...
Embodiment 2
[0064] The preparation of embodiment 2 Geobacillus stearothermophilus
[0065] Geobacillus stearothermophilus (provided by Shanghai Enzyme Bioconsumables Co., Ltd.) was activated at 34°C for 24 hours, and then inoculated in the enriched culture medium, the composition of which was 6.0 g of sodium chloride and 6.0 g of peptone , beef powder 3.0g, distilled water 1000mL, pH 7.0, cultured at 34°C and 230rpm for 24 hours, then inoculated the enriched strains in the aerobic fermentation medium with a volume fraction of 5% for aerobic fermentation Cultivation, the composition of the fermentation medium is: glucose 45g, ammonium nitrite 7.0g, dipotassium hydrogen phosphate 14.0g, sodium dihydrogen phosphate 3.0g, magnesium sulfate 0.6g, manganese sulfate 0.04g, yeast extract 0.6g, distilled water 1000mL , pH 7.0, the culture conditions are: temperature 34°C, rotation speed 200rpm, ventilation rate 1.2L / L min, the air passed through a 0.45μm filter membrane for filter sterilization, a...
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