Composite microbial agent for treating black and odor water bodies, obtaining method and use method thereof
A technology of compound microbial agent and black and odorous water body, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problem of low efficiency of microbial agent treatment, poor specificity of river and pit sewage treatment, etc. problem, to achieve the effect of high efficiency and specificity
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[0036] The black and odorous water body was collected from a pond in Qingguang Town, Beichen District, Tianjin, and the strains were isolated and screened from the raw water:
[0037] (1) Specific screening medium to screen Bacillus. Leave the sewage (derived from black and odorous water) for 24 hours, mix the supernatant with sterile water, and press 10 -4 、10 -5 and 10 -6 Doubling gradient dilution to make bacterial suspension, add it to specific screening medium (liquid), and cultivate for 24 hours; dilute the expansion culture solution by 10 3 、10 4 、10 5 and 10 6 double, spread evenly on the improved specific screening medium (solid) plate, and cultivate for 12 hours; pick a single colony, and repeatedly carry out liquid culture and streak separation on a single strain to obtain pure strains of Bacillus megaterium F5 and thermophilic fatty soil Bacillus F32; LB test tube slant and cryopreserved.
[0038] Specific screening medium: per 1000mL water, glucose 2g, star...
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