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Green alga glycosyl microcapsule biological agent and preparation method and application thereof

A biological agent and chlorophyllose-based technology, applied in the field of microorganisms, can solve the problems of low survival rate of inorganic fertilizer compound, inability to adapt to stress environment, pollution of agricultural ecological environment, etc., achieve good technical effect and market application prospect, improve Survival rate, pollution reduction effect

Inactive Publication Date: 2015-04-01
青岛海大生物集团股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the promotion and protection of crop growth mainly depends on the use of pesticides and chemical fertilizers, but the excessive abuse of pesticides and chemical fertilizers will cause soil compaction, soil salinization and heavy metal pollution, seriously pollute the agricultural ecological environment, and endanger human health
[0003] In recent years, under the permission of national policies, some pesticides and chemical fertilizers have been gradually replaced by microecological mixtures. Microecological preparations have both the effect of fertilizer and the effect of pesticides, combining plant protection and plant nutrition. At the same time, microbial fertilizers There are also disadvantages such as poor symbiosis of various strains, inability to adapt to the stress environment, and low survival rate when compounded with inorganic fertilizers.

Method used

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  • Green alga glycosyl microcapsule biological agent and preparation method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1, the preparation of green algae sugar-based microcapsule biological agent

[0033] The chlorophyll-based microcapsule biological agent provided by the present invention includes Geobacillus stearothermophilus, Bacillus floridae, methylotrophic Bacillus and Bacillus sime, and the bacteria content of the four strains is: thermophilic 20% Geobacillus stearatus, 10% Bacillus floridae, 10% methylotrophic Bacillus, and 60% Bacillus sime, calculated according to the percentage of viable bacteria, the active bacteria are wrapped in enteromorpha polysaccharide as the main component In the capsule wall material, the green algae sugar-based microcapsule biological agent is made.

[0034] Extraction and Preparation of Enteromorpha Polysaccharide

[0035] Enteromorpha raw powder can be divided into soluble and insoluble components through hot water extraction, and most of the soluble components are soluble polysaccharides. The soluble fraction was pretreated with hydr...

Embodiment 2

[0059] Example 2, the application of chlorophyll-based microcapsule biological bacteria agent in agricultural and animal husbandry waste and Enteromorpha

[0060] Corn stalks: 500 million / gram of solid bacterial agent dry powder after dilution can be applied or sprayed, and 5g of the original powder can be used per ton; the effect is better when used with 3kg of urea.

[0061] Sheep manure: After dilution, 500 million / gram solid bacterial agent dry powder can be applied or sprayed, and the dosage is 500g per ton.

[0062] Enteromorpha powder: after dilution, 500 million / gram of solid bacterial agent dry powder can be applied or sprayed, and the dosage is 500g per ton.

[0063] Using the decomposing bacterial agent of the present invention to carry out aerobic fermentation and decomposing of agricultural and animal husbandry waste and Enteromorpha can be piled up with a length, width and height of more than 1 meter, and the pile is turned over once every 4 days to facilitate ox...

Embodiment 3

[0065] Embodiment 3, compounding of chlorophyll-based microbial bacterial agent and macroelement water-soluble fertilizer

[0066] The bacterium powder and urea, ammonium sulfate, potassium nitrate, potassium dihydrogen phosphate, and trace elements boron, manganese, copper, and zinc are prepared in a certain proportion to prepare nitrogen, phosphorus, and potassium. The nutritional composition is 12-3-18, and the number of effective viable bacteria is ≥ 400 million / g high-efficiency compound biological macroelement water-soluble fertilizer. The product was placed at room temperature, and the preparation was counted on plates at regular intervals to detect the stability of the number of viable bacteria. The experimental results are shown in Table 1.

[0067] Table 1 Activity degradation law of chlorophyll-based microbial inoculum at room temperature

[0068] time 0d 60d 120d 300d 360d 420d 480d 540d Effective bacteria count (100 million / g) 2017 2003 ...

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Abstract

The invention discloses a green alga glycosyl microcapsule biological agent and its preparation method and application. Four strains Geobacillus stearothermophilus, Bacillus vallismortis, Bacillus methylotrophicus and Bacillus siamensis are mixed and compounded in proportion; and by a microencapsulation technology which is most effective and practical for protection of strain activity, the active strains are wrapped in a capsule wall substance with Enteromorpha prolifera polysaccharide being the main component. Thus, the green alga glycosyl microcapsule biological agent is prepared. In addition, the invention specifically provides a preparation method of the green alga glycosyl microcapsule biological agent. The green alga glycosyl microcapsule biological agent provided by the invention is pollution-free and can achieve the efficacy of cooperative usage of a fertilizer and a pesticide. The technology is simple and costs are low. The environment is protected. The product has high disease resistance and stress resistance. Survival rate is enhanced. The product is easy for industrial production.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a green algae sugar-based microcapsule biological agent and its preparation method and application. Background technique [0002] my country is a large agricultural country, and the yield and quality of crops are related to the national economy and people's livelihood. At present, the promotion and protection of crop growth mainly depends on the use of pesticides and chemical fertilizers, but the excessive abuse of pesticides and chemical fertilizers causes soil compaction, soil salinization and heavy metal pollution, which seriously pollutes the agricultural ecological environment and endangers human health. [0003] In recent years, under the permission of national policies, some pesticides and chemical fertilizers have been gradually replaced by microecological mixtures. Microecological preparations have both the effect of fertilizer and the effect of pesticides, combin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/10C05F17/00C05G3/00C09K17/00C12R1/07C12R1/01
CPCY02W30/40
Inventor 单俊伟翟熙伦王鹏王海华王真真乔来艳
Owner 青岛海大生物集团股份有限公司
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