Method for obtaining geobacillus stearothermophilus spores

Abstract

The invention relates to a method for obtaining geobacillus stearothermophilus spores. The method comprises the following steps of inoculating the geobacillus stearothermophilus into a liquid seed culture medium; performing culture to obtain seed liquid; inoculating the seed liquid into a liquid spore producing inducing culture medium to be subjected to induced culture; performing induction to produce the spores. The method has the advantages that the culture of the seed cells and the induction of the spores are both performed in the liquid culture; the culture and induction efficiency are greatly improved; the time is shortened; the labor operation is reduced; the high spore number and the high spore yield can be obtained in 36 to 48 hours.

Description

technical field [0001] The present invention relates to the field of culture of spore-forming bacteria, more particularly, to a method for obtaining spores of Geobacillus stearothermophilus. Background technique [0002] The best growth temperature of Geobacillus stearothermophilus is 55-60℃, the spores produced by it are non-pathogenic, non-pyrogenic, non-toxic, and the resistance to pressure steam is the strongest among most microorganisms, so it is often used It can be used as an indicator bacteria to check the disinfection effect of high pressure steam sterilization. [0003] At present, there are solid and semi-solid culture methods for preparing the spore suspension of Geobacillus stearothermophilus in the prior art, and the spore suspension is prepared by culturing in solid or semi-solid medium, eluting, heat treatment, separation and constant volume. There are many defects in this method, such as long culturing time to obtain spores, labor and time-consuming extract...

AI Technical Summary

Problems solved by technology

There are many defects in this method, such as long cultivation time to obtain spores, labor-intensive and time-consuming extraction of spores, many steps, complicated operation

In addition, in solid or semi-solid medium, the growth space of bacteria is limited, the distribution is uneven, the growth rate is slow, and the yield of spores is low; the liquid fermentation method in the prior art has many operation steps and the cultivation method is complicated

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