Method for obtaining geobacillus stearothermophilus spores
A technology of Geobacillus and thermophilic fat, which is applied in the field of spore-bearing bacteria culture, can solve the problems of uneven distribution, slow growth, long spore culture time, etc., and achieves the effects of reducing manual operation, improving efficiency and shortening time.
Active Publication Date: 2017-12-15
湖北省微工生物技术研究有限公司
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There are many defects in this method, such as long cultivation time to obtain spores, labor-intensive and time-consuming extraction of spores, many steps, complicated operation
In addition, in solid or semi-solid medium, the growt
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[0025] The principles and features of the present invention will be described below with reference to examples. The examples are only used to explain the present invention, but not to limit the scope of the present invention.
[0026] 1. Determination of bacterial concentration-dependent absorption spectra
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The invention relates to a method for obtaining geobacillus stearothermophilus spores. The method comprises the following steps of inoculating the geobacillus stearothermophilus into a liquid seed culture medium; performing culture to obtain seed liquid; inoculating the seed liquid into a liquid spore producing inducing culture medium to be subjected to induced culture; performing induction to produce the spores. The method has the advantages that the culture of the seed cells and the induction of the spores are both performed in the liquid culture; the culture and induction efficiency are greatly improved; the time is shortened; the labor operation is reduced; the high spore number and the high spore yield can be obtained in 36 to 48 hours.
Description
technical field [0001] The present invention relates to the field of culture of spore-forming bacteria, more particularly, to a method for obtaining spores of Geobacillus stearothermophilus. Background technique [0002] The best growth temperature of Geobacillus stearothermophilus is 55-60℃, the spores produced by it are non-pathogenic, non-pyrogenic, non-toxic, and the resistance to pressure steam is the strongest among most microorganisms, so it is often used It can be used as an indicator bacteria to check the disinfection effect of high pressure steam sterilization. [0003] At present, there are solid and semi-solid culture methods for preparing the spore suspension of Geobacillus stearothermophilus in the prior art, and the spore suspension is prepared by culturing in solid or semi-solid medium, eluting, heat treatment, separation and constant volume. There are many defects in this method, such as long culturing time to obtain spores, labor and time-consuming extract...
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Login to view more IPC IPC(8): C12N3/00C12N1/20C12R1/07
CPCC12N1/20C12N3/00
Inventor 罗茜刘婷邓小华郑巧双余微高奋李震宇潘笃杰付晓超
Owner 湖北省微工生物技术研究有限公司
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