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Straw-decomposing inoculant

A technology of straw decomposing agent and strain, which is applied in the directions of organic fertilizers, microorganisms, fungi, etc., can solve the problems of low utilization rate of straw and long natural composting time, etc., and achieve the effect of improving soil urease activity and cellulase activity.

Inactive Publication Date: 2014-04-16
DALIAN SANKE BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the time required for natural composting is relatively long, and the utilization rate of straw is low. Therefore, it is urgent to develop an efficient microbial compound agent to improve the decomposing speed and decomposing rate of straw.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 Degradation of straw to prepare fertilizer

[0042] (1) Preparation of straw decomposing agent

[0043] In the preparation method of the above-mentioned straw decomposing agent, according to the method of steps (1)-(2), the culture liquid of each strain is obtained, and the culture liquid of each strain is inoculated in 300ml strain fermentation medium according to the mass ratio of 5%, Cultivate as follows to obtain Reagent A, Reagent B, Reagent C, Reagent D, and Reagent E, respectively.

[0044] Bacillus subtilis (Bacillus subtilis) CICC10088 was cultivated in the strain fermentation medium at 28°C and 200r / min shaking for 3 days, and all the fermentation liquid was collected as reagent A.

[0045] Aspergillus niger (Aspergillus niger) CICC2436 was cultivated in the strain fermentation medium at 28°C and 200r / min shaking for 3 days, and all the fermentation broth was collected as reagent B.

[0046] Trichoderma viride (Trichoderma viride) ACCC30449 was cul...

Embodiment 2

[0053] Example 2 Determination of Water Soluble Carbon (WSC) and Total Organic Nitrogen of Straw Organic Fertilizer

[0054] The WSC of the straw organic fertilizer 1 prepared in Example 1 was measured by the potassium dichromate volumetric method, and the measurement of the total organic nitrogen of the straw organic fertilizer 1 was measured by H 2 SO 4 -H 2 o 2 Digestion, Kjeldahl method. Experiments were repeated three times.

[0055] The measurement results of WSC and total organic nitrogen showed that the WSC (water-soluble carbon) of straw organic fertilizer 1 was 7.25g / kg, the ratio of WSC and total organic nitrogen was 0.75, and the T value (end point C / N to initial C / N Ratio) is 0.45, which meets the requirement of maturity and is at least 15 days earlier than the normal compost fermentation time. The above values ​​are the average of three repetitions.

Embodiment 3

[0056] Field experiment of embodiment 3 straw organic fertilizer

[0057] In the field experiment, the spinach field and the cabbage field were respectively selected, and a completely random block design was adopted, and the two fields were divided into three blocks, 1, 2, and 3, respectively. Experimental group 3 used straw organic fertilizer 1 as a base fertilizer at the sowing or transplanting stage, control group 1 applied straw directly, and control group 2 did not use any fertilizer, and the area of ​​each plot was 10m 2 . The field management of the control group and the experimental group was the same except that the fertilization was different.

[0058] The contents of alkaline nitrogen, available phosphorus, potassium and organic matter, soil urease activity and cellulase activity in the soil were measured 40 days after transplanting.

[0059] Determination of soil alkaline nitrogen by alkaline solution diffusion method; use 0.5mol / L NaHCO 3 Determination of soil ...

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Abstract

The invention discloses a straw-decomposing inoculant which comprises the following active components: bacillus subtilis, aspergillus niger, trichoderma viride, phanerochaete chrysosporimn and geobacillus stearothermophilus. In the straw-decomposing inoculant, the total content of effective viable bacteria is (1-2)* 10<8> / ml. With adoption of the straw-decomposing inoculant, the fertilizer prepared from straws can be rapidly degraded, and the normal compost fermentation is performed in advance for at least 15 days. When the straw organic fertilizer prepared from the straw-decomposing inoculant is used as the base fertilizer, alkali-hydrolyzale nitrogen, rapid available phosphorus, potassium and organic contents in the soil are higher than that in the soil in which straws are directly utilized and no fertilizer is applied, and the urease activity and cellulase activity in the soil are obviously higher than that in the soil in which straws are directly utilized and no fertilizer is applied.

Description

technical field [0001] The invention relates to a decomposing agent, in particular to a decomposing agent for degrading straw and a preparation method thereof. Background technique [0002] Crop straw is a huge resource with the characteristics of large quantity, variety and wide distribution. According to statistics, the annual output of straw in my country is about 700 million tons, but the utilization efficiency of straw is extremely low, and most of the straw is incinerated and discarded, causing serious environmental pollution and waste of resources. At present, straw is mostly used in agriculture by returning it directly to the field. However, due to the slow decomposition rate of straw returning to the field in cold soil and the decrease in soil temperature, which affects the growth of crops, the proportion of straw returning to the field has always been relatively low. Returning straw to the field through composting is undoubtedly the best way to solve these problem...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14C05F11/08C12R1/125C12R1/01C12R1/69C12R1/885C12R1/645
Inventor 顾艳丽崔国臣周悦崔昌娜
Owner DALIAN SANKE BIO ENG
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