High-temperature kitchen waste degradation microbial agent and preparing and using method thereof

A microbial agent and kitchen waste technology, applied in the biological field, can solve problems such as potential safety hazards, and achieve the effects of rapid degradation, simple use, and high biological safety

Inactive Publication Date: 2015-04-22
SHANDONG URBAN MINING REGENERATION RESOURCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Since the optimum growth temperature for most pathogenic bacteria, such as Staphylococcus aureus and Escherichia coli, is around 35°C, if the food waste is fermented by mesophilic microbial treatment, there may be non-inactivated bacteria in the output. pathogenic bacteria, there is a certain degree of safety hazard

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Single-plant culture and high-density expansion culture were carried out on the anaerobic Bacillus nubihalogen, Bacillus liquefactionus, Geobacillus thermodenitrophilus, and Geobacillus stearothermophilus, and finally the single bacteria obtained after cultivation were used Diatomaceous earth is used for adsorption to obtain a single dry bacterial powder of each bacterial strain.

[0039] 1. Preparation of Nubi anaerobic Bacillus haloteri single bacteria powder

[0040] (1), inoculate the anaerobic bacillus nubihalogenes on the slant of the test tube after purification into a conical flask filled with a liquid activation medium with a volume ratio of 10-20%; the solvent of the liquid activation medium is distilled water , the solute components and their concentrations are: peptone 8g / L, beef extract 3g / L, yeast extract 5g / L, soluble starch 17g / L, sodium chloride 4g / L, and the pH value of the liquid activation medium is 7.0; The liquid activated medium of Nubi anaerobic...

Embodiment 2

[0054] Single-plant culture and high-density expansion culture were carried out on the anaerobic Bacillus nubihalogen, Bacillus liquefactionus, Geobacillus thermodenitrophilus, and Geobacillus stearothermophilus, and finally the single bacteria obtained after cultivation were used Diatomaceous earth is used for adsorption to obtain a single dry bacterial powder of each bacterial strain.

[0055] 1. Preparation of Nubi anaerobic Bacillus haloteri single bacteria powder

[0056] (1), inoculating the anaerobic bacillus nubihalogenes on the slant of the test tube after purification to the conical flask that is equipped with a liquid activation medium with a volume ratio of 15%; the solvent of the liquid activation medium is distilled water, solute The components and their concentrations are: peptone 9g / L, beef extract 4g / L, yeast extract 3g / L, soluble starch 25g / L, sodium chloride 6g / L, the pH value of the liquid activation medium is 7.0; The liquid activated medium of anaerobic ...

Embodiment 3

[0070] Single-plant culture and high-density expansion culture were carried out on the anaerobic Bacillus nubihalogen, Bacillus liquefactionus, Geobacillus thermodenitrophilus, and Geobacillus stearothermophilus, and finally the single bacteria obtained after cultivation were used Diatomaceous earth is used for adsorption to obtain a single dry bacterial powder of each bacterial strain.

[0071] 1. Preparation of Nubi anaerobic Bacillus haloteri single bacteria powder

[0072] (1), inoculating the anaerobic bacillus nubihalogenes on the purified test tube slant to a conical flask containing a 10% liquid activation medium by volume; the solvent of the liquid activation medium is distilled water, solute The components and their concentrations are: peptone 12g / L, beef extract 2g / L, yeast extract 4g / L, soluble starch 15g / L, sodium chloride 5g / L, the pH value of the liquid activation medium is 7.1; The liquid activated medium of anaerobic bacillus haloteri was cultured for 24 hour...

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Abstract

The invention discloses a high-temperature kitchen waste degradation microbial agent and a preparing and using method thereof. A microbial strain comprises, by weight, 15-30 parts of nuby land anoxybacillus, 10-25 parts of liquefaction thermophilic bacillus, 20-40 parts of hot nitrogen bacillus and 25-50 parts of geobacillus stearothermophilus. Compared with the prior art, the high-temperature kitchen waste degradation microbial agent has the beneficial effects that as for kitchen waste with different integrants, reasonable matching on single bacterium powder can be carried out, and single bacterial strains are cooperated for degrading the kitchen waste in a collaborative mode; the number of effective viable bacteria in the single bacterium powder in the composite microbial agent is large, and when the microbial agent is used for degrading the kitchen waste, only a small number of microbial agents is needed for sufficiently and rapidly degrading the kitchen waste; components in the microbial agent are high-temperature cultures, the optimum fermentation temperature ranges from 55 DEG C to 65 DEG C, pathogenic bacteria such as escherichia coli and staphylococcus aureus in the kitchen waste can be effectively inactivated in the fermentation process, and the biosecurity is high.

Description

technical field [0001] The invention belongs to the field of biological technology, and relates to a microbial bacterial agent for high-temperature degradation of kitchen waste and its preparation and use methods. Background technique [0002] Food waste has the characteristics of high oil, high salt, and high protein content. If it is not handled properly, it is easy to breed mosquitoes and flies, rot and deteriorate, emit foul smell, and breed mosquitoes and flies. Some food waste is even used by criminals to refine " "gutter oil" and feeding "swill pigs" have caused serious impacts on the environment and people's health. [0003] At present, the biological treatment methods of kitchen waste mainly include medium-temperature microbial treatment and high-temperature microbial treatment. [0004] The fermentation temperature of the medium-temperature microbial treatment method is generally 25 to 40°C, and the fermentation temperature of the high-temperature method can reach...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09B3/00C12R1/01C12R1/07
CPCB09B3/00C12N1/20
Inventor 李宗武马玉莹贺保国卢乐健孙加安
Owner SHANDONG URBAN MINING REGENERATION RESOURCE
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