Microbial agent, greening waste soil remediation agent and application thereof

Abstract

The invention relates to the technical field of microbe applications and in particular relates to a microbial agent, a greening waste soil remediation agent as well as a preparation method and application thereof. Each milliliter of the agent comprises 1*10<10-12> trichoderma asperellum WL spores, 1*10<10-12> geobacillus stearothermophilus viable spores and 1*10<10-12> CFU bacillus amyloliquefaciens. The agent comprises the trichoderma asperellum WL separated by the inventor, and the strain has an effect of enriching heavy metal materials, has a cellulose degradation function and can be well used for greening waste degradation and soil remediation. The invention further provides a greening waste soil remediation agent. The greening waste soil remediation agent can achieve the effects of overcoming the absorption and digestion difficulty of the conventional urban greening wastes, remediating salt and alkali lands and removing heavy metals and petrochemical pollution sources in contaminated lands.

Description

technical field

[0001] The invention relates to the technical field of microbial application, in particular to a microbial bacterial agent, a greening garbage soil remediation agent and a preparation method and application thereof. Background technique

[0002] Compared with air and water pollution, soil remediation is more difficult. Su Dechun, a member of the Ministry of Agriculture's expert group on the prevention and control of heavy metal pollution in agricultural product areas and a professor at the School of Resources and Environment of China Agricultural University, said that not only is soil pollution a serious hazard, but it is also different from some organic pollutants in that heavy metals in soil cannot be degraded, and it is very difficult to separate them from the soil. , very difficult to fix. Soil heavy metal pollutants are mainly mercury, lead, copper, zinc, chromium, nickel, tin and metal arsenic. At present, there are many soil treatment technologies, e...

Examples

Embodiment 1

[0039] Present embodiment provides the microbial inoculant containing Trichoderma aculeatus spore powder and preparation method thereof, and the method comprises the steps:

[0040] 1), the preserved bacterial classification is first received on the PDA medium plate, activated and cultivated at 28°C until sporulation, and the resulting spore powder is sieved (200 mesh), to obtain the Trichoderma aculeatus spore powder;

[0041] Take the spore powder and make it into a concentration of 1×10 11 spore / mL suspension to obtain the first microbial liquid inoculant.

[0042]Wherein, the configuration method contained in the PDA medium mentioned above is as follows: Weigh 200g of potatoes, wash, peel and cut into small pieces, add 1000ml of water to boil for half an hour, filter with gauze, add 10-20g of glucose, fully dissolve Set the volume to 1000ml, and sterilize at 121 degrees for 30 minutes.

[0043] 2), conventionally culturing and counting Geobacillus stearothermophilus and ...

Embodiment 2

[0047] In this example, the preparation operation is the same as in Example 1. The difference from Example 1 is that in this example, in addition to adding Geobacillus stearothermophilus, Bacillus subtilis and Thermoanaerobacillus metalophilus are additionally added. , Dispospora thermophila, and Bacillus licheniformis, the above-mentioned bacteria are added after the composite bacteria liquid formed according to the proportion of the bacteria liquid volume in equal proportions, and the bacteria content is 1-9×10 10 pieces / ml.

Embodiment 3

[0049] In this example, the preparation operation is the same as in Example 1. The difference from Example 1 is that in this example, in addition to adding Geobacillus stearothermophilus, Lactobacillus plantarum, Lactobacillus delbrueckii, Lactobacillus delbrueckii, Methanothermophilic micrococcus, white thermophilic actinomycetes and citric acid bacteria, the above-mentioned bacteria are added in the composite bacteria liquid formed according to the proportion of the bacteria liquid volume ratio, and the bacteria content is 1-9×10 10 pieces / ml.

technical field

[0001] The invention relates to the technical field of microbial application, in particular to a microbial bacterial agent, a greening garbage soil remediation agent and a preparation method and application thereof. Background technique

[0002] Compared with air and water pollution, soil remediation is more difficult. Su Dechun, a member of the Ministry of Agriculture's expert group on the prevention and control of heavy metal pollution in agricultural product areas and a professor at the School of Resources and Environment of China Agricultural University, said that not only is soil pollution a serious hazard, but it is also different from some organic pollutants in that heavy metals in soil cannot be degraded, and it is very difficult to separate them from the soil. , very difficult to fix. Soil heavy metal pollutants are mainly mercury, lead, copper, zinc, chromium, nickel, tin and metal arsenic. At present, there are many soil treatment technologies, e...

Examples

Embodiment 1

[0039] Present embodiment provides the microbial inoculant containing Trichoderma aculeatus spore powder and preparation method thereof, and the method comprises the steps:

[0040] 1), the preserved bacterial classification is first received on the PDA medium plate, activated and cultivated at 28°C until sporulation, and the resulting spore powder is sieved (200 mesh), to obtain the Trichoderma aculeatus spore powder;

[0041] Take the spore powder and make it into a concentration of 1×10 11 spore / mL suspension to obtain the first microbial liquid inoculant.

[0042]Wherein, the configuration method contained in the PDA medium mentioned above is as follows: Weigh 200g of potatoes, wash, peel and cut into small pieces, add 1000ml of water to boil for half an hour, filter with gauze, add 10-20g of glucose, fully dissolve Set the volume to 1000ml, and sterilize at 121 degrees for 30 minutes.

[0043] 2), conventionally culturing and counting Geobacillus stearothermophilus and ...

Embodiment 2

[0047] In this example, the preparation operation is the same as in Example 1. The difference from Example 1 is that in this example, in addition to adding Geobacillus stearothermophilus, Bacillus subtilis and Thermoanaerobacillus metalophilus are additionally added. , Dispospora thermophila, and Bacillus licheniformis, the above-mentioned bacteria are added after the composite bacteria liquid formed according to the proportion of the bacteria liquid volume in equal proportions, and the bacteria content is 1-9×10 10 pieces / ml.

Embodiment 3

[0049] In this example, the preparation operation is the same as in Example 1. The difference from Example 1 is that in this example, in addition to adding Geobacillus stearothermophilus, Lactobacillus plantarum, Lactobacillus delbrueckii, Lactobacillus delbrueckii, Methanothermophilic micrococcus, white thermophilic actinomycetes and citric acid bacteria, the above-mentioned bacteria are added in the composite bacteria liquid formed according to the proportion of the bacteria liquid volume ratio, and the bacteria content is 1-9×10 10 pieces / ml.

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