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Composite thermophilic microbial agent and method for synergistically decomposing polystyrene in composting

A compound bacterial agent, polystyrene plastic technology, applied in the field of biotechnology and environment, can solve the problems such as no, and achieve the effect of promoting degradation and accelerating oxidation

Pending Publication Date: 2018-10-30
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far there is no research report on thermophilic plastic-degrading bacteria and the use of thermophilic microorganisms to synergistically degrade plastics in composting

Method used

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  • Composite thermophilic microbial agent and method for synergistically decomposing polystyrene in composting
  • Composite thermophilic microbial agent and method for synergistically decomposing polystyrene in composting
  • Composite thermophilic microbial agent and method for synergistically decomposing polystyrene in composting

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Preparation of Compound Thermophilic Bacteria

[0043] 1) According to 2%, the concentration is 10 9 Cfu / mL Thermaerobacter composti FAFU014 shake flask bacterial liquid was inoculated into the seed tank with THERMAEROBACTER TY (JCM medium 734) medium, 70-75°C, ventilation volume 20-100m 3 / h, stirring speed 180-220 rev / min, fermentation time 16 hours, obtain Thermaerobacter compostiFAFU014 seed liquid;

[0044] 2) According to 2%, the concentration is 10 9 cfu / mL Thermus thermophilus FAFU013 shake flask bacterial liquid was inoculated into the seed tank containing DSMZ Medium 74 medium, 70°C, ventilation volume 20-100m 3 / h, stirring speed 180-220 rev / min, fermentation time 16 hours, obtain Thermus thermophilus FAFU013 seed liquid;

[0045] 3) According to 2%, the concentration is 10 9 cfu / mL Geobacillus stearothermophilus FAFU011 shake flask bacterial solution was inoculated into seed tanks containing DSMZ Medium 220 medium respectively, at 60°C, with an air flow ...

Embodiment 2

[0057] Preparation of thermophilic bacteria

[0058] 1) According to the method of Example 1, respectively prepare Thermus thermophilus FAFU013 and Geobacillus stearothermophilus FAFU011 seed solution;

[0059] 2) Take the concentrated sludge of a sewage treatment plant in Fujian (see Table 3 for the basic properties of the sludge), in the fermenter, adjust the sludge to a solid content of 4%, add 0.2% foam enemy, 2g / L yeast powder , 0.5g / L ammonium chloride, 0.5g / L sucrose, 0.5g / L soluble starch, 0.3g / L potassium dihydrogen phosphate, adjust the pH to 7.0, stir well, sterilize at 100°C for 45 minutes, cool to 75°C spare;

[0060] 3) First inoculate the Thermaerobacter composti FAFU014 seed liquid into the fermentation medium at 1.5% inoculum amount, and inoculate at 75°C with an air flow of 20-100m 3 / h, stirring speed 180-220 rpm, pre-fermentation for 18 hours; then inoculate Geobacillus stearothermophilus FAFU011 seed liquid at 2.5%, control the fermentation temperature a...

Embodiment 3

[0070] Preparation of thermophilic bacteria

[0071] (1) According to the method of Example 1, respectively prepare Thermus thermophilus FAFU013 and Geobacillus stearothermophilus FAFU011 seed solutions;

[0072] (2) Take the concentrated sludge of a sewage treatment plant in Fujian (the basic properties of the sludge are shown in Table 5). In the fermenter, adjust the sludge to a solid content of 4%, add 0.2% foam enemy, 2g / L Yeast powder, 0.5g / L ammonium chloride, 0.5g / L sucrose, 0.5g / L soluble starch, 0.3g / L potassium dihydrogen phosphate, adjust the pH to 7.0, stir well, sterilize at 100°C for 45 minutes, cool to Standby at 70°C;

[0073](3) First inoculate the Thermus thermophilus FAFU013 seed liquid at 2%, control the fermentation temperature at 70°C, and carry out pre-fermentation for 16 hours; finally inoculate the Geobacillus stearothermophilus FAFU011 seed liquid at 2.5%, control the fermentation temperature at 65°C, and carry out post-fermentation for 20 hours. Af...

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Abstract

The invention discloses a composite thermophilic microbial agent and a method for synergistically decomposing polystyrene in composting. The composite thermophilic microbial agent contains at least two of thermophilic bacteria strains of Thermus thermophilus FAFU013, Thermaerobacter composti FAFU014 and Geobacillus stearothermophilus FAFU011. The composite thermophilic microbial agent is thermophilic, and can directly use nutrient substances in municipal sludge, excrements of livestocks, agricultural residues, kitchen waste and other organic solid wastes to grow and breed, the compost temperature can be quickly increased to be above 70 DEG C, the oxidation of polystyrene can be accelerated in composting, and the degradation of polystyrene waste plastic is prompted under the combined effectof the composite thermophilic microbial agent.

Description

technical field [0001] The invention relates to the fields of biotechnology and environmental technology, in particular to a composite microbial agent and a method for synergistically degrading polystyrene in composting. Background technique [0002] As an important polymer material, plastic has excellent properties such as light weight, low price, high strength and easy processing, and is widely used in various fields of production and life. my country is a big country in plastic production and consumption. In 2013, the world consumed 299 million tons of plastics, of which 24.8% came from China. Polystyrene (PS) plastic is widely used in the production of packaging containers, daily necessities, electronic products and foamed plastics, accounting for 7.1% of the total plastic production, and the demand for PS in my country is growing at a rate of 5.9% every year. However, polystyrene, with its high molecular weight and high stability, is one of the most difficult plastics ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05G3/00C12R1/01
CPCC12N1/20C05F3/00C05G3/00C05F5/002C05F11/00C05F11/08
Inventor 陈志赵文琪谢胜佳周顺桂
Owner FUJIAN AGRI & FORESTRY UNIV
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