Spent mushroom substrate high-temperature decomposition composite bacterial agent and preparation method thereof

A compound bacterial agent, pyrolysis technology, used in biochemical equipment and methods, microorganism-based methods, bacteria, etc.

Active Publication Date: 2014-03-05
神农架天润生物科技有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are no reports and applications of rapid and effective decomposing bacteria agents specially developed for bacterial chaff

Method used

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  • Spent mushroom substrate high-temperature decomposition composite bacterial agent and preparation method thereof
  • Spent mushroom substrate high-temperature decomposition composite bacterial agent and preparation method thereof
  • Spent mushroom substrate high-temperature decomposition composite bacterial agent and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0030] (1) The 8 strains of bacteria were cultured separately, and the culture methods were as follows:

[0031] Strain preservation number ATCC51176, the culture of DSM3670Aeribacillus pallidus, will be composed of glucose 1.0g, peptone 7.5g, beef extract 5.0g, yeast powder 2.5g, casamino acids 2.5g, sodium chloride 5.0g, distilled water 1000.0ml composition medium, pH The value was adjusted to 8.5; 60 ℃ protected from light static culture for 48h.

[0032] For the cultivation of strain preservation number DSM2027, ATCC7954 Geobacillus stearothermophilus, a culture medium consisting of 5.0g of sodium chloride, 10.0g of beef extract, 10.0g of peptone, and 1000.0mL of distilled water was adjusted to a pH value of 7.2; statically cultured at 60°C in the dark for 48 hours.

[0033] For the cultivation of strain preservation number DSM7064 Brevibacillus thermoruber, adjust the pH value to 7.0 in a culture medium consisting of 15.0 g of glucose, 5.0 g of yeast powder, 0.2 g of calc...

example 2

[0050] Example 2, same as Example 1, the difference is,

[0051] (1) The mixed fermentation ratio of the 8 strains of bacteria is: Aeribacillus pallidus (strain preservation number ATCC51176, DSM3670), Geobacillus stearothermophilus (strain preservation number DSM2027, ATCC7954), Brevibacillus thermoruber (strain preservation number DSM7064), Clostridium stercorarium subsp.thermolacticum (strain collection number ATCC43739, DSM2910), Clostridium thermocellum (strain collection number NCIB10682, ATCC27405), Desulfotomaculum nigrificans (strain collection number DSM574, ATCC19858), Geobacillus thermoglucosidasius (strain collection number DSM21625), Symbiophilum8 the The cell number content in the strain is specifically 12%, 14%, 3%, 14%, 17%, 10%, 14% and 16%.

[0052] The composite bacterial agent prepared in this example is shown in Table 2 for its ability to decompose different bacterial chaff.

[0053] Table 2 Composite bacteria agent's ability to decompose different bacte...

example 3

[0055] Example 3, the same as Example 1, the difference is,

[0056] (1) Among the complex bacteria, Aeribacillus pallidus (strain preservation number ATCC51176, DSM3670), Geobacillus stearothermophilus (strain preservation number DSM2027, ATCC7954), Brevibacillus thermoruber (strain preservation number DSM7064), Clostridium stercorarium subsp. , DSM2910), Clostridium thermocellum (strain collection number NCIB10682, ATCC27405), Desulfotomaculum nigrificans (strain collection number DSM574, ATCC19858), Geobacillus thermoglucosidasius (strain collection number DSM21625), Symbiobacterium thermophilum (strain collection number DSM21625), and Symbiobacterium thermophilum (strain collection number 28) with specific content of 16 cells %, 15%, 4%, 11%, 18%, 7%, 11%, and 18%.

[0057] The composite bacterial agent prepared in this example is shown in Table 3 for its ability to decompose different bacterial chaff.

[0058] Table 3 Decomposition ability of compound microbial agent to ...

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Abstract

The present invention relates to a spent mushroom substrate high-temperature decomposition composite bacterial agent, wherein eight thermophilic bacteria such as Aeribacillus pallidus, Geobacillus stearothermophilus, Brevibacillus thermoruber, Clostridium stercorarium subsp. thermolacticum, Clostridium thermocellum, Desulfotomaculum nigrificans and the like are subjected to mixing and mixing culture according to a certain cell number content ratio, composite bacterial agent preparation is performed when decomposition ability is high, different spent mushroom substrates are adopted as absorption carriers, peptone, yeast powder and the like are added, the bacterial liquid is repeatedly sprayed, absorption fermentation is performed, and drying at a temperature of 60 DEG C is performed to obtain the composite bacterial agent. The composite bacterial agent can be used for spent mushroom substrate feeds, spent mushroom substrate fertilizers and energy source conversion and other uses.

Description

technical field [0001] The invention relates to the technical field of decomposition and comprehensive utilization of agricultural by-products, in particular to a composite bacterial agent for pyrolysis of fungus chaff. Background technique [0002] Mushroom bran is the residue of the culture medium after edible fungus fruiting bodies are harvested by using wood chips, cottonseed hulls, straw and other lignocellulosic raw materials. It is commonly known as edible fungus cultivation waste and fungus residue. In recent years, the edible fungus industry has developed rapidly, and the amount of fungus chaff resources is increasing. At present, most of the fungus chaff resources have not been effectively utilized, resulting in waste of resources and environmental pollution. Therefore, solving the problem of fungus chaff utilization is extremely important for the recycling of agricultural resources and the control of environmental pollution. The main component of fungus chaff is ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/145C12R1/01
Inventor 郭鹏程薇周明陈明利高虹王晨杨德李露薛淑静史德芳
Owner 神农架天润生物科技有限责任公司
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