Preparation method of composite microbial agent for kitchen waste composting
A technology of composite microbial agent and food waste, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problem that food waste cannot be processed in time
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[0106] The present invention will be described in further detail below in combination with specific embodiments.
[0107] In the present invention, before preparing the composite microbial bacterial agent, it is necessary to prepare various types of medium in advance, such as slant medium, separation and screening medium, mesophilic microbial bacterial agent, high-temperature microbial bacterial agent, etc. as described below.
[0108] Culture medium preparation
[0109] 1. Preparation of slant medium
[0110] 1-A. Bacteria preservation:
[0111] Beef extract 3.0g, peptone 10.0g, sodium chloride (NaCI) 15.0g, agar 20g, distilled water 1000mL, pH7.2-7.4.
[0112] 1-B. Preservation of cellulolytic bacteria:
[0113] Sodium carboxymethylcellulose (CMC-Na) 20.0g, disodium hydrogen phosphate (Na 2 HPO 4 ) 2.5g, potassium dihydrogen phosphate (KH 2 PO 4 ) 1.5g, peptone 2.5g, yeast extract 0.5g, agar 20.0g, distilled water 1000mL, pH7.2-7.4.
[0114] 2. Separation and screeni...
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