The invention relates to the technical field of microorganisms, in particular to high-temperature-resistant
bacillus megaterium and application thereof, the high-temperature-resistant
bacillus megaterium is named as LYGW-R2, and the preservation number is CCTCC (China Center for Type Culture Collection) NO: M2024962. Soil is collected from a field test field, the soil is obtained through
enrichment culture,
Congo red dyeing and
aniline blue
fading screening, the degradation bacterial strains are sequenced by determining the activity of degradation
bacteria cellulase, hemicellulase and ligninase, performing a
filter paper strip disintegration test and
straw degradation rate research and adopting 16s rDNA (ribosomal deoxyribonucleic acid), and the content of the degradation bacterial strains is determined. And finally, the high-temperature-resistant
bacillus megaterium LYGW-R2 with a relatively high degradation rate on
lignin and
cellulose is selected out. The bacillus megaterium is used for
straw decomposition, can still keep excellent
biological activity at a relatively high composting temperature, generates
cellulose degrading
enzyme and
lignin degrading
enzyme, and can promote degradation of
lignin and
cellulose in a
straw composting process, shorten a composting period and improve
compost maturity and straw
utilization rate.