High-temperature-resistant bacillus megaterium and application thereof

Abstract

The invention relates to the technical field of microorganisms, in particular to high-temperature-resistant bacillus megaterium and application thereof, the high-temperature-resistant bacillus megaterium is named as LYGW-R2, and the preservation number is CCTCC (China Center for Type Culture Collection) NO: M2024962. Soil is collected from a field test field, the soil is obtained through enrichment culture, Congo red dyeing and aniline blue fading screening, the degradation bacterial strains are sequenced by determining the activity of degradation bacteria cellulase, hemicellulase and ligninase, performing a filter paper strip disintegration test and straw degradation rate research and adopting 16s rDNA (ribosomal deoxyribonucleic acid), and the content of the degradation bacterial strains is determined. And finally, the high-temperature-resistant bacillus megaterium LYGW-R2 with a relatively high degradation rate on lignin and cellulose is selected out. The bacillus megaterium is used for straw decomposition, can still keep excellent biological activity at a relatively high composting temperature, generates cellulose degrading enzyme and lignin degrading enzyme, and can promote degradation of lignin and cellulose in a straw composting process, shorten a composting period and improve compost maturity and straw utilization rate.

Description

Technical Field

[0001] The invention relates to the technical field of microorganisms, and in particular to a thermostable Bacillus megaterium and an application thereof. Background Art

[0002] According to statistics, the annual output of crop straw exceeds 800 million tons, and the overall output is showing a slow upward trend. Straw is rich in organic substances such as cellulose, hemicellulose, lignin, protein, and a large number of nutrients such as nitrogen, phosphorus, potassium, calcium, magnesium, sulfur, silicon, etc. It is a high-quality agricultural resource.

[0003] However, due to the tight and complex structure, high degree of polymerization and strong resistance to decomposition of lignin, cellulose and hemicellulose in straw, a large amount of straw is not fully utilized. In the process of using microorganisms to degrade straw, there are problems such as slow degradation of lignocellulose components, long fermentation cycle, incomplete use of materials, and low degree of maturity. Due to the high temperature during composting, most microorganisms are not suitable for survival.

[0004] Therefore, the prior art still needs to be improved and developed. Summary of the invention

[0005] In view of the above-mentioned deficiencies in the prior art, the object of the present invention is to provide a thermostable Bacillus megaterium and its application, aiming to solve the problems of high temperature during straw decomposition, which makes it difficult for microorganisms to survive and leads to slow degradation of lignocellulose components.

[0006] The technical solution of the present invention is as follows:

[0007] A thermostable Bacillus megaterium is named as LYGW-R2, and its deposit number is CCTCC NO: M2024962.

[0008] A bacterial agent containing the thermostable Bacillus megaterium.

[0009] The bacterial agent, wherein the bacterial agent also includes auxiliary materials and / or carriers.

[0010] A method for preparing a bacterial agent containing thermostable Bacillus megaterium comprises the following steps:

[0011] activating the thermostable Bacillus megaterium to obtain activated thermostable Bacillus megaterium;

[0012] The activated thermostable Bacillus megaterium is inoculated into a PDA liquid culture medium to obtain a bacterial agent.

[0013] The method for preparing the bacterial agent, wherein the activation treatment step comprises:

[0014] The thermostable Bacillus megaterium is inoculated on a PDA solid culture medium by a spread plate inoculation method, and cultured at 29° C.-31° C. for 23 h-25 h to complete the activation treatment.

[0015] In the method for preparing the bacterial agent, the activated thermostable Bacillus megaterium is inoculated into the PDA liquid culture medium in an inoculation amount of 1%-4% by volume.

[0016] The preparation method of the bacterial agent, wherein the number of viable bacteria in the bacterial agent is 5.0×10 -9 cfu / ml-6.0×10 -9 cfu / ml.

[0017] Application of a thermostable Bacillus megaterium in straw composting.

[0018] Application of thermostable Bacillus megaterium in lignin degradation and / or cellulose degradation.

[0019] Beneficial effects: The present invention provides a thermostable Bacillus megaterium and its application, wherein the thermostable Bacillus megaterium is named LYGW-R2 and has a preservation number of CCTCC NO: M2024962. The present invention collects soil in a field test field, obtains the soil through enrichment culture, Congo red staining, and aniline blue fading screening, measures the activity of cellulose, hemicellulose, and lignin degradation bacteria, performs filter paper strip disintegration tests, and studies the straw degradation rate, and uses 16s rDNA to sequence the degradation strain, and finally selects the thermostable Bacillus megaterium LYGW-R2 with a high degradation rate for lignin and cellulose, and preserves it in the China Center for Type Culture Collection, with a preservation number of CCTCC NO: M2024962. The Bacillus megaterium is used in straw decomposition and can maintain excellent biological activity at relatively high composting temperatures, produce cellulose-degrading enzymes and lignin-degrading enzymes, and can promote the degradation of lignocellulose during straw composting, shorten the composting cycle, and improve the compost maturity and straw utilization rate, that is, accelerate the straw decomposition process; in addition, the strain has good high temperature resistance and has a significant effect on straw degradation. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 This is a biofilm of the bacterial plaque obtained in Example 1;

[0021] Figure 2 The phylogenetic tree constructed based on 16s rDNA sequence in Example 1;

[0022] Figure 3 This is a rendering of the filter paper strip degradation experiment in Example 3;

[0023] Figure 4 is the activity data graph of carboxymethyl cellulase;

[0024] Figure 5 is the activity data diagram of β-D glucosidase;

[0025] Figure 6 is the activity data graph of manganese peroxidase;

[0026] Figure 7 This is the data chart of straw degradation rate. DETAILED DESCRIPTION

[0027] The present invention provides a thermostable Bacillus megaterium and its application. To make the purpose, technical scheme and effect of the present invention clearer and more specific, the present invention is further described in detail below. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0028] It will be understood by those skilled in the art that, unless otherwise defined, all terms (including technical and scientific terms) used herein have the same meaning as generally understood by those skilled in the art in the art to which the present invention belongs. It should also be understood that terms such as those defined in general dictionaries should be understood to have meanings consistent with the meanings in the context of the prior art, and will not be interpreted with idealized or overly formal meanings unless specifically defined as herein.

[0029] The present invention provides a high-temperature resistant Bacillus megateritun, which is named LYGW-R2. The strain has been deposited in China Center for Type Culture Collection (CCTCC for short, address: Wuhan University, Wuhan, China) on May 23, 2024, with a preservation number of CCTCC NO: M2024962, and is classified and named: Bacillus megateritun LYGW-R2 (Priestia megateritun LYGW-R2).

[0030] In this embodiment, the thermostable Bacillus megaterium LYGW-R2 is obtained by collecting soil in a field test field, and then screened by enrichment culture, Congo red staining, and aniline blue fading. Then, the degradation strains are sequenced by 16s rDNA by measuring the activity of cellulose, hemicellulose, and lignin enzymes, filter paper strip disintegration test, and straw degradation rate. Finally, a thermostable Bacillus megaterium LYGW-R2 with a high degradation rate of lignin and cellulose is selected and preserved in the China Center for Type Culture Collection, with a preservation number of CCTCC NO: M2024962. The thermostable Bacillus megaterium is used in straw decomposition, and can still maintain excellent biological activity at a higher composting temperature, produce cellulose degrading enzymes and lignin degrading enzymes, promote the degradation of lignocellulose in the process of straw composting, shorten the composting cycle, improve the composting maturity and the utilization rate of straw, that is, accelerate the decomposition process of straw; and the strain has good high temperature resistance and obvious effect on straw degradation.

[0031] In some embodiments, the 16s rDNA sequence of the thermostable Bacillus megaterium is as shown in SEQ ID NO.1.

[0032] In some embodiments, the colony morphology of the thermostable Bacillus megaterium is characterized by: the colony morphology on the enriched culture medium is round, the positive and reverse colors are both yellow, transparent and with regular edges, a smooth surface, glossy and glossy, and the degree of dryness and wetness is slightly wet.

[0033] In addition, the present invention also provides a bacterial agent containing the thermostable Bacillus megaterium.

[0034] In this embodiment, when the bacterial agent containing the thermostable Bacillus megaterium is used for straw decomposition, it can still maintain excellent biological activity at a relatively high composting temperature, produce cellulose degrading enzymes and lignin degrading enzymes, accelerate the decomposition process of the straw, and the strain has good high temperature resistance and a significant effect on straw degradation.

[0035] In some embodiments, the bacterial agent further comprises auxiliary materials and / or carriers, which can provide survival conditions for the Bacillus megaterium and ensure its activity.

[0036] In addition, the present invention also provides a method for preparing a thermostable Bacillus megaterium agent, comprising the steps of:

[0037] Step S10: activating the thermostable Bacillus megaterium to obtain activated thermostable Bacillus megaterium;

[0038] Step S20: inoculating the activated thermostable Bacillus megaterium into a PDA liquid culture medium to obtain a bacterial agent.

[0039] In this embodiment, the thermostable Bacillus megaterium can be made into a bacterial agent by activation treatment and inoculation. The bacterial agent can be used in straw decomposition. It can still maintain excellent biological activity at a higher composting temperature, produce cellulose degrading enzymes and lignin degrading enzymes, promote the degradation of lignocellulose during straw composting, shorten the composting cycle, and improve the compost maturity and straw utilization rate; that is, the bacterial agent can accelerate the decomposition process of straw, and the strains contained in the strain have good high temperature resistance and obvious advantages in straw degradation effect.

[0040] In some embodiments, the activation treatment step includes: inoculating the thermostable Bacillus megaterium on a PDA solid culture medium by a spread plate inoculation method, and culturing at 29° C.-31° C. for 23 h-25 h to complete the activation treatment.

[0041] Specifically, the high-temperature resistant Bacillus megaterium strain is taken out from the ultra-low temperature refrigerator for activation: inoculated on a PDA solid culture medium by a spread plate inoculation method, and cultured at 29° C.-31° C. for 23-25 ​​hours; after the activation, a sterilized inoculation loop is used to gently scrape a portion of the bacterial film, and inoculated into a PDA liquid culture medium (without agar) to prepare a liquid bacterial agent.

[0042] In some embodiments, after the liquid bacterial agent is prepared, the liquid bacterial agent can be added to the constructed carrier to prepare a bacterial agent co-body.

[0043] In some embodiments, the carrier includes but is not limited to bentonite, diatomaceous earth, sodium alginate-gelatin, and the like.

[0044] In some embodiments, the preparation step of the PDA solid culture medium includes: weighing 200g of peeled potato pieces, boiling for 30min, collecting the filtrate, adding ultrapure water to 1.0L, then adding 20.0g of glucose and 20.0g of agar, adjusting the pH value to 6.9-7.1, and sterilizing in a high-pressure steam sterilizer to obtain a PDA solid culture medium. Compared with the PDA solid culture medium, the PDA liquid culture medium does not contain agar.

[0045] In some embodiments, the activated thermostable Bacillus megaterium is inoculated into the PDA liquid culture medium in an inoculation amount of 1%-4% by volume.

[0046] In a preferred embodiment, the activated thermostable Bacillus megaterium is inoculated into the PDA liquid culture medium in an inoculation amount of 1% by volume.

[0047] In some embodiments, the number of viable bacteria in the bacterial agent is 5.0×10 -9 cfu / ml-6.0×10 -9cfu / ml.

[0048] In addition, the present invention also provides an application of the thermostable Bacillus megaterium in straw composting.

[0049] In addition, the present invention also provides an application of the thermostable Bacillus megaterium in lignin degradation and / or cellulose degradation.

[0050] In this embodiment, the thermostable Bacillus megaterium is used in straw decomposition, and can still maintain excellent biological activity at a higher composting temperature, produce cellulose degrading enzymes and lignin degrading enzymes, accelerate the decomposition process of the straw, and the strain has good high temperature resistance and obvious straw degradation effect. Further, the thermostable Bacillus megaterium can promote the degradation of lignocellulose during straw composting, shorten the composting cycle, and improve the compost maturity and straw utilization rate.

[0051] The following examples are further given to illustrate the present invention in detail. It should also be understood that the following examples are only used to further illustrate the present invention and cannot be understood as limiting the scope of protection of the present invention. Some non-essential improvements and adjustments made by those skilled in the art based on the above content of the present invention belong to the scope of protection of the present invention.

[0052] The culture medium required for the following examples includes:

[0053] Alkali lignin solid culture medium: alkaline lignin 5g, (NH4)2SO4 2g, K2HPO4 1g, KH2PO4 1g, MgSO4·7H2O 0.2g, NaCl 1.5g, H2O 1L, agar powder 10g, pH=7.5, sterilization at 121℃ for 20min.

[0054] Sodium carboxymethyl cellulose (CMC-Na) solid culture medium: CMC-Na 5.0 g, (NH4)2SO4 2.0 g, K2HPO4 1.0 g, NaNO3 3.0 g, KCl 0.5 g, MgSO4 0.5 g, agar 10.0 g, H2O 1 L, humidity 50%, high pressure sterilization at 121 ° C for 20 min.

[0055] Enrichment culture medium: alkaline lignin liquid culture medium, sodium carboxymethyl cellulose liquid culture medium; compared with solid culture medium, liquid culture medium does not contain agar.

[0056] Aniline blue rescreening culture medium: yeast powder 15g, glucose 15g, K2HPO4 0.5g, KNO3 1.0g, MgSO4 0.5g, aniline blue 0.1g, H2O 1L, agar powder 10g, pH=7.5.

[0057] PDA medium: 1.0 L potato boiled liquid, 20.0 g glucose, 20.0 g agar (not included in liquid medium). Potato boiled liquid: weigh 200 g peeled potato pieces, boil for 30 min, collect the filtrate and add water to 1.0 L.

[0058] Straw powder culture medium: straw powder 20g, K2HPO4 1g, NaCl 0.1g, MgSO4 0.3g, (NH4)2SO4 0.5g, FeCl3 0.01g, CaCl2 0.1g, H2O 1L, pH=7.2.

[0059] Filter paper strip degradation medium: filter paper strip 0.2 g, K2HPO4 1 g, NaCl 0.1 g, MgSO4 0.3 g, (NH4)2SO4 0.5 g, FeCl3 0.01 g, CaCl2 0.1 g, H2O 1 L, pH = 7.2.

[0060] Example 1

[0061] This example separates and identifies the Bacillus megaterium LYGW-R2 strain, including the following steps:

[0062] Weigh 5 g of soil sample into a conical flask containing glass beads, add 45 mL of enrichment medium, and shake at 180 r min in an oscillator. -1 After shaking for 72 hours, an enriched soil suspension was obtained.

[0063] Use sterile water to dilute the soil suspension in a gradient manner. -2 , 10 -3 , 10 -4 , 10 -5 , 10 -6 100 μL of each soil suspension was evenly spread on PDA medium, and 3 parallels were made for each concentration. The medium was inverted and cultured at 50°C for 3 days.

[0064] When colonies grow in the culture medium, pick out a single colony and enrich it in a liquid culture medium. Purify the obtained colony isolate on the corresponding plate culture medium using the three-zone line method until a single colony is obtained. Record the morphology, color, size, colony edge, gloss, transparency, surface, degree of protrusion, etc. of the single colony. Number the obtained single colonies and store them in a -80°C refrigerator for later use. The morphological characteristics of the colonies are shown in Table 1, and the actual picture of the colonies is shown in Figure 1 shown.

[0065] Table 1 Morphological characteristics of colonies

[0066] shape Positive Color Invert Color size edge smooth transparent luster Wet and dry surface round yellow yellow middle rule √ √ √ Slightly wet smooth

[0067] 1) Hydrolysis zone determination test of the screened Bacillus megaterium LYGW-R2 strain

[0068] The strains were screened and cultured on sodium carboxymethyl cellulose solid medium for 3 days. -1 After staining with Congo red for 15 min, the staining solution was discarded and 1 mol L -1 The cells were eluted with NaCl solution, and the ratio of the transparent zone diameter to the colony diameter was measured with a ruler; the cells were cultured with aniline blue rescreening medium for 3 days, and the ratio of the transparent zone diameter to the colony diameter was measured with a ruler. Specifically, the ratio of the transparent zone diameter to the colony diameter is shown in Table 2.

[0069] Table 2 Ratio of transparent circle diameter to colony diameter

[0070] Experiment Name Transparent ring diameter (mm) Colony diameter (mm) Transparent circle diameter / colony diameter Congo red staining 11.0 8.0 1.375 Aniline blue fading 9.0 7.5 1.200

[0071] 2) Identification of Bacillus megaterium LYGW-R2 strain

[0072] The 16s rDNA of Bacillus megaterium LYGW-R2 strain was sequenced to determine the genus of Bacillus megaterium LYGW-R2 strain. The DNA of the strain was extracted by sampling column method and magnetic bead method. The forward primer was 27F: 5'-AGAGTTTGATCCTGGCTCAG-3'; the reverse primer was 1492R: 5'-TACGGCTACCTTGTTACGACTT-3'. PCR reaction system: PCR Mix 21μL, Primer F (5p) and Primer R (5p) 1μL each, template DNA template amount (2ngμL -1 )2μL, the total system volume was 25μL. The amplification program was: 96℃ preheating for 5min; 96℃20s, 62℃30s, 72℃30s, 35 cycles; 72℃ final extension for 10min, and then maintained at 4℃. The PCR product was purified by gel excision and gene sequencing was completed. The sequencing results were compared with NCBI-BLAST and analyzed by MEGA11 software. The neighbor-joining method was used to construct a phylogenetic tree; the phylogenetic tree is shown in Figure 2 shown.

[0073] The 16s rDNA sequence of Bacillus megaterium was obtained as shown in SEQ ID NO.1

[0074] Example 2

[0075] In this example, the enzyme activity secreted by Bacillus megaterium LYGW-R2 strain was detected, as follows:

[0076] The enzymatic activity of the screened Bacillus megaterium LYGW-R2 was determined, the carboxymethyl cellulase activity was determined by the 3,5-dinitrosalicylic acid method, the β-D-glucosidase activity was determined by the p-nitrophenyl glucoside hydrolysis method, and the manganese peroxidase activity was determined by the manganese sulfate spectrophotometry method. The enzyme activity data table is shown in Table 3.

[0077] Table 3 Enzyme activity data

[0078] type Enzyme activity (U / ml) Carboxymethyl Cellulase 267 β-D-glucosidase 512 Manganese peroxidase 2.849

[0079] Example 3

[0080] This example mainly studies the lignin and cellulose degradation effects of Bacillus megaterium LYGW-R2, including the following:

[0081] 1) Filter paper strip degradation experiment

[0082] The bacterial solution was inoculated into 50 mL of filter paper strip degradation medium at a 1% inoculation volume and cultured for 5 days. The degradation degree of the filter paper strip was recorded and observed. The degradation effect was judged according to the degree of damage to the filter paper. The disintegration of the filter paper strip is shown in Table 4; "+" indicates that the edge of the filter paper has expanded; "++" indicates that the filter paper has expanded as a whole and has bent downward; "+++" indicates that the filter paper is in an indeterminate shape. The effect diagram of the LYGW-R2 filter paper strip degradation experiment (marked as R2) is shown in Table 4. Figure 3 shown.

[0083] Table 4 Disintegration of filter paper strips

[0084]

[0085]

[0086] 2) Straw degradation rate test

[0087] Weigh 2g of straw powder into a nylon bag and place it in a 250ml triangular flask. Inoculate the single strain obtained by screening into 50mL of sterilized straw powder medium at a 1% inoculum. Repeat each treatment 3 times. Meanwhile, a control (CK) without any bacterial strain inoculation was used. The culture was incubated at 30℃ and 150r min. -1 The straw powder was fully rinsed with sterile water, dried at 80℃ to constant weight, and weighed. The degradation rate of the straw powder was calculated by weight loss method. After 15 days, the degradation rate was as high as 25.60%.

[0088] Example 4

[0089] In this example, the high temperature resistance of Bacillus megaterium LYGW-R2 strain was measured, as follows:

[0090] High temperature resistance experimental data: Bacillus megaterium LYGW-R2 strain was screened out under 50℃ culture conditions. The high temperature resistance of Bacillus megaterium LYGW-R2 strain was tested to explore its ultimate tolerance temperature. +: indicates the number of bacteria, "++" indicates the number of colonies>50; "+" indicates the number of colonies<50; "-" indicates that the number of colonies is 0. The results are shown in Table 5. It can be concluded that LYGW-R2 is inactivated at 80℃ and stops working. It still has good activity at 70℃ and can play a role.

[0091] Table 5 High temperature resistance test data

[0092] Temperature ℃ LYGW-R2 60 ++ 70 + 80 -

[0093] Comparative Example 1

[0094] For comparison, two strains R3 and R5 were isolated from the same farmland soil as described in Example 1 and identified according to conventional methods. The identification results showed that R3 was a Bacillus subtilis strain and R5 was a Bacillus licheniformis strain.

[0095] Taking R3 and R5 as comparative examples, the same enzyme activity test, straw degradation rate test, filter paper strip degradation test, and high temperature resistance test were performed on them. The enzyme activity data comparison table is shown in Table 6, and the activity data of carboxymethyl cellulase is shown in Table 7. Figure 4 As shown, the activity data of β-D glucosidase is shown in Figure 5 As shown in the figure, the activity data of manganese peroxidase is as follows Figure 6 The straw degradation situation is shown in Table 7, and the straw degradation rate data is shown in Figure 7 The comparison of the degradation effects of the filter paper strips is shown in Table 8; the comparison of the high temperature resistance performance is shown in Table 9.

[0096] Table 6 Enzyme activity data comparison table

[0097]

[0098] Table 7 Straw degradation status

[0099] serial number Straw degradation rate (%) R3 23.22 R5 24.13 LYGW-R2 25.60

[0100] Table 8 Comparison of filter paper strip degradation effects

[0101]

[0102]

[0103] Table 9 High temperature resistance performance comparison table

[0104] Temperature ℃ LYGW-R2 R3 R5 60 ++ ++ ++ 70 + + - 80 - - -

[0105] In summary, the present invention provides a thermostable Bacillus megaterium and its application, wherein the thermostable Bacillus megaterium is named LYGW-R2, and the deposit number is: CCTCC NO: M2024962. The present invention collects soil in a field test field, obtains lignocellulose-degrading bacteria through enrichment culture and Congo red staining and aniline blue fading initial screening, measures the cellulose, hemicellulose and ligninase activities of the degrading bacteria, performs filter paper strip disintegration tests and studies the straw degradation rate, and uses 16srDNA to sequence the degradation strain, and finally selects the thermostable Bacillus megaterium LYGW-R2 with a high degradation rate for lignin and cellulose, and deposits it in the China Center for Type Culture Collection, with the deposit number of CCTCC NO: M2024962. The Bacillus megaterium is used in straw decomposition and can maintain excellent biological activity at relatively high composting temperatures, produce cellulose-degrading enzymes and lignin-degrading enzymes, and can promote the degradation of lignocellulose during straw composting, shorten the composting cycle, and improve the compost maturity and straw utilization rate, that is, accelerate the straw decomposition process; in addition, the strain has good high temperature resistance and has a significant effect on straw degradation.

[0106] It should be understood that the application of the present invention is not limited to the above examples. For ordinary technicians in this field, improvements or changes can be made based on the above description. All these improvements and changes should fall within the scope of protection of the claims attached to the present invention.

Claims

1. A thermostable Bacillus megaterium, characterized in that The thermostable Bacillus megaterium is named as LYGW-R2, and its deposit number is: CCTCC NO: M2024962.

2. A bacterial agent containing the thermostable Bacillus megaterium as claimed in claim 1.

3. The bacterial agent according to claim 2, characterized in that The bacterial agent also includes auxiliary materials and / or carriers.

4. A method for preparing a bacterial agent containing thermostable Bacillus megaterium, characterized in that: Includes steps: activating the thermostable Bacillus megaterium as claimed in claim 1 to obtain an activated thermostable Bacillus megaterium; The activated thermostable Bacillus megaterium is inoculated into a PDA liquid culture medium to obtain a bacterial agent.

5. The method for preparing the bacterial agent according to claim 4, characterized in that: The activation treatment step comprises: The thermostable Bacillus megaterium is inoculated on a PDA solid culture medium by a spread plate inoculation method, and cultured at 29° C.-31° C. for 23 h-25 h to complete the activation treatment.

6. The method for preparing the bacterial agent according to claim 4, characterized in that: The activated thermostable Bacillus megaterium is inoculated into the PDA liquid culture medium in an inoculation amount of 1%-4% by volume.

7. The method for preparing the bacterial agent according to claim 4, characterized in that: The number of viable bacteria in the bacterial agent is 5.0×10 -9 cfu / ml-6.0×10 -9 cfu / ml.

8. Use of the thermostable Bacillus megaterium as claimed in claim 1 in straw composting.

9. Use of the thermostable Bacillus megaterium as claimed in claim 1 in lignin degradation and / or cellulose degradation.

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