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Method for preparing high-efficiency complex microbial inoculum for processing human and animal excreta

A technology of composite microbial agent and human and animal feces, applied in the field of ecological environmental protection, can solve the problems of unsatisfactory treatment effect, complicated production and preparation, and inability to popularize and use, and achieve the effect of low cost, simple preparation method, and rapid removal

Inactive Publication Date: 2009-12-02
上海新张卫生用品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] With the development of science and technology, there are currently many domestic and foreign resource-based treatment methods focusing on human and animal manure and artificially cultivated microorganisms used in them. However, most of these microbial strains have problems such as too single, too complicated production and high cost, resulting in The processing effect is not ideal and cannot be promoted

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] The high-efficiency composite microbial agent for treating human and animal manure is composed of Bacillus subtilis, Bacillus licheniformis, Geobacillus stearothermophilus, Streptococcus thermophilus, Geotrichum candidum, Aspergillus niger and Streptomyces thermoamylase, the ratio of various microorganisms Bacillus subtilis: Bacillus licheniformis: Geobacillus stearothermophilus: Streptococcus thermophilus: Geotrichum candidum: Aspergillus niger: Streptomyces thermoamylase=3:2:5:3:3:3:4.

[0015] The bacterial species is cultivated by the following method: the bacterial agent is cultured on a primary slant, a primary shake flask, and a secondary shake flask in sequence, and then the bacteria are transplanted into a solid medium at a room temperature of 35° C. for 96 hours.

[0016] When in use, the cultured high-efficiency complex microbial flora is inoculated and colonized with biological fillers in a special container at a temperature of 35°C, and the fillers are place...

Embodiment 2

[0019] The high-efficiency composite microbial agent for treating human and animal manure is composed of Bacillus subtilis, Bacillus licheniformis, Geobacillus stearothermophilus, Streptococcus thermophilus, Geotrichum candidum, Aspergillus niger and Streptomyces thermoamylase, the ratio of various microorganisms Bacillus subtilis: Bacillus licheniformis: Geobacillus stearothermophilus: Streptococcus thermophilus: Geotrichum candidum: Aspergillus niger: Streptomyces thermoamylase=3:2:5:3:3:3:4.

[0020] The culture method of bacterial agent is with embodiment 1.

[0021] Put fresh chicken droppings and bird droppings in a fermenter, add sawdust at a weight ratio of 30% and mix evenly, adjust the water content to 45%, and obtain pretreated materials. At 35°C, add the above-mentioned microbial agent to it with an inoculation amount of 5% by weight, stir regularly, ferment for 7 days, the water content drops to about 20%, and the appearance is gray with a slight smell of distille...

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Abstract

The invention relates to a complex microbial inoculum for processing human and animal excreta efficiently, which consists of bacillus subtilis, bacillus licheniformis, geobacillus stearothermophilus, streptococcus thermophilus, geotrichum candidum, aspergillus niger and heat amylase streptomyces. A preparation method comprises the following steps of first-stage shake flask culture, second-stage shake flask culture, and third-stage solid medium culture of the complex microbial inoculum for the strains. The preparation method is simple with low use cost, saves resources, can be widely applied to stationary type or wheeled lavatories, and accords with the policy of sustainable development.

Description

technical field [0001] The invention belongs to the field of ecological environment protection, and relates to a preparation method of a high-efficiency composite microbial bacterial agent for treating human and animal feces. Background technique [0002] The toilet plays an important role in people's daily life. It receives, accumulates and eliminates most of the pollutants excreted in human's daily physiological activities every day. After these manures are discharged into urban sewers, they become the main source of pollutants in domestic sewage and are also an important part of urban sewage pollutants. [0003] With the development of science and technology, there are currently many domestic and foreign resource-based treatment methods focusing on human and animal manure and artificially cultivated microorganisms used in them. However, most of these microbial strains have problems such as too single, too complicated production and high cost, resulting in The processing ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/14C02F11/02C12R1/125C12R1/10C12R1/07C12R1/46C12R1/685C12R1/465C12R1/645
Inventor 马志恒
Owner 上海新张卫生用品有限公司
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