Quick detection method for antibiotic residue in raw milk

Abstract

The invention discloses a quick detection method for antibiotic residue in raw milk and belongs to the technical field of biology. According to the quick detection method disclosed by the invention, frozen, dried and stored geobacillus stearothermophilus is added into nutrient gravy agar culture medium to be activated and cultured; the activated geobacillus stearothermophilus is inoculated into phosphate buffer solution to obtain suspension liquid; the geobacillus stearothermophilus suspension liquid is added into bromcresol purple peptone culture medium to obtain test culture medium; the test culture medium is evenly mixed with raw milk to be detected according to volume ratio of (1:2) to (1:3) to be cultured in water bath in 75 DEG C to 80 DEG C for 2.0h to 2.2h, and color change of culture medium is observed. According to the quick detection method disclosed by the invention, system color change reaction time is greatly shortened by adjusting water bath culture temperature to 75 DEG C and coordinating corresponding reasonable volume ratio of the test culture medium to the raw milk, the time is shortened by about 20%, and detecting efficiency is further improved.

Description

technical field [0001] The invention relates to a rapid detection method for antibiotic residues in raw milk, belonging to the field of biotechnology. Background technique [0002] Antibiotics in raw milk refer to parent compounds of veterinary drugs and their metabolites contained in any edible part of animal products, as well as impurities related to veterinary drugs. Due to the irrational use of therapeutic drugs in the feeding process of lactating animals, antibiotic residues in raw milk are widely present in their milk. At present, antibiotic residues in raw milk are still one of the most important issues in the global control of milk safety, especially for China, where the incidence of dairy cow diseases is high, the number of special pharmaceuticals for dairy cows is relatively small, and the medication of dairy cows is chaotic, resulting in the lack of treatment for some diseases. The effect is unsatisfactory, and the drug inspection of raw milk sometimes exceeds th...

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problems of long detection time and low detection efficiency in the prior art, the present invention provides a detection method for antibiotic residues in raw milk, and the adopted technical scheme is as follows:

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