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Preparation method of composite bacterial agent for sludge composting and application thereof

A compound bacterial agent and composting technology, applied in the field of microbial fermentation, can solve the problems of unsatisfactory fertilizer effect, single microbial effect, and difficulty in wide application, and achieve the effects of improving crop stress resistance, shortening composting time, and promoting growth and reproduction.

Active Publication Date: 2019-02-26
合肥市东方美捷分子材料技术有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In recent years, microbial agents have been used in sludge composting, but there are still many problems, such as the single effect of some microorganisms, complicated operation, and unsatisfactory fertilizer effects, which make it difficult to be widely used.

Method used

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  • Preparation method of composite bacterial agent for sludge composting and application thereof
  • Preparation method of composite bacterial agent for sludge composting and application thereof
  • Preparation method of composite bacterial agent for sludge composting and application thereof

Examples

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preparation example Construction

[0023] The preparation method of each bacterial strain in the described composite bacterial agent is as follows:

[0024] (1) The preparation of the Bacillus uplander bacteria liquid is as follows: pick the Bacillus uplander strains and inoculate them into a solid slant culture, place them in the medium for cultivation, the culture temperature is 37° C., and the culture time is 24 hours, and wait for the slant culture After the colonies grow out of the base, inoculate them into the liquid medium for shaker culture. The shaker speed is 150-180r / min, the culture temperature is 35-37°C, and the culture time is 36-48h. When the number of viable bacteria reaches 1×10 8 When CFU / mL, the preparation of the bacterial solution is completed; the solid slant medium is made by mixing the following raw materials in the weight ratio: glucose 5g, peptone 5g, beef extract 3g, NaCl 5g, agar 15g, supplemented with distilled water to 1L, pH =7, sterilized at 121°C for 30 minutes and cooled for ...

Embodiment 1

[0031] After mixing the bacterial solutions of Bacillus highlander, Streptomyces violaceum, Geobacillus stearothermophilus, Bacillus subtilis and Bacillus licheniformis at a volume ratio of 0.5:0.5:0.2:0.5:0.2, the fermentation after high temperature sterilization Inoculate with a medium volume ratio of 8%, ferment at 35°C for 5 days, when the number of viable bacteria reaches 1×10 8 When the CFU / mL is above, the culture is complete. The fermentation medium is made by mixing the following raw materials in weight ratio: brown sugar 15-25g, peptone 5-10g, beef extract 3-5g, NaCl2-5g, KH 2 PO 4 0.75-1.5g, MgSO 4 .7H 2 O 0.5-1g, K 2 HPO 4 0.8-1g, vitamin B1 10-15mg, potato extract 1L (take 200g peeled potatoes, cut into small pieces, add 1L of water and boil for 30min, filter out the potato pieces, make up the filtrate to 1L), PH=7, extinguish at 121℃ Cool for 30 minutes before use.

Embodiment 2

[0033] After mixing the bacterial solutions of Bacillus highlander, Streptomyces violaceum, Geobacillus stearothermophilus, Bacillus subtilis and Bacillus licheniformis at a volume ratio of 0.8:0.8:0.5:0.8:0.5, the fermentation after high temperature sterilization Inoculate with a medium volume ratio of 10%, ferment at 35°C for 5 days, when the number of viable bacteria reaches 1×10 8 When the CFU / mL is above, the culture is complete. The fermentation medium is made by mixing the following raw materials in weight ratio: brown sugar 15-25g, peptone 5-10g, beef extract 3-5g, NaCl2-5g, KH 2 PO 4 0.75-1.5g, MgSO 4 .7H 2 O 0.5-1g, K 2 HPO 4 0.8-1g, vitamin B1 10-15mg, potato extract 1L (take 200g peeled potatoes, cut into small pieces, add 1L of water and boil for 30min, filter out the potato pieces, make up the filtrate to 1L), PH=7, extinguish at 121℃ Cool for 30 minutes before use.

[0034] This embodiment is the best embodiment.

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Abstract

The invention discloses a preparation method of a composite bacterial agent for sludge composting and application thereof. The preparation method comprises the following steps: separately culturing bacillus altitudinis, purple streptomyces variabilis, bacillus stearothermophilus, bacillus subtilis and bacillus licheniformis, then mixing according to a volume ratio of bacterial liquid to obtain mixed bacterial liquid, then inoculating a fermentation medium with the mixed bacterial liquid, and performing enlargement culture to obtain the composite bacterial agent for sludge composting. The composite bacterial agent is mainly applied to high-temperature sludge composting, raises the fermentation temperature fast, maintains high temperature for a long time, can shorten the composting time, reduces the moisture content of a composted material, is low in production cost, environmentally friendly, pollution-free, excellent in performance and high in efficiency, and has relatively good economic benefits and social benefits.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, and in particular relates to a preparation method and application of a composite microbial agent for sludge composting. Background technique [0002] With the continuous acceleration of my country's urbanization level, the amount of urban sewage treatment continues to increase, and the treatment of a large amount of excess sludge generated during sewage treatment has become a major problem in urban development. At present, the methods used to treat sludge in my country mainly include sludge dehydration, dry incineration, sanitary landfill, composting, etc. Among them, sludge composting technology is usually mixed with straw, wood chips, fly ash, poultry manure and other auxiliary materials before adding Aerobic composting with microbial agents is an efficient, green and harmless sludge resource treatment method. The traditional sludge composting method mainly uses the indigenous mi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F15/00C05F17/00C12R1/07C12R1/465C12R1/01C12R1/125C12R1/10
CPCC05F3/00C05F17/00C12N1/20C05F5/002C05F7/00C05F9/00C05F11/00C05F11/08Y02W30/40
Inventor 朱守诚桑建伟黄家榜杨宏星褚维发孙庆红程鹏飞
Owner 合肥市东方美捷分子材料技术有限公司
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