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Geobacillus caldoxylosilyticus strain producing cyclodextrin glycosyltransferase

A technology of glucosyltransferase and Geobacillus, applied in the direction of transferase, microbial-based methods, bacteria, etc.

Active Publication Date: 2014-08-13
INST OF SOIL & FERTILIZER FUJIAN ACADEMY OF AGRI SCI
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Problems solved by technology

The inventors found a strain producing high-temperature CGTase, and there is no report of other strains of the same species of Geobacillus caldoxylosilyticus producing this enzyme. The crude enzyme solution is obtained through liquid fermentation, and the crude enzyme solution can efficiently catalyze soluble starch to produce cyclodextrin

Method used

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  • Geobacillus caldoxylosilyticus strain producing cyclodextrin glycosyltransferase
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  • Geobacillus caldoxylosilyticus strain producing cyclodextrin glycosyltransferase

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Embodiment 1

[0023] 1. Isolation of the strain

[0024] (1) Take 10 g soil sample in 80 mL sterilized ddH 2 Shake well with O, apply gradient dilution on NA solid plate medium, and cultivate overnight in a constant temperature incubator at 60°C to 65°C;

[0025] (2) Pick colonies with differences in shape, color, and size in step (1), and purify them by streaking on solid plates respectively, and after purification three times, keep the bacteria on the slant of the test tube;

[0026] (3) Pick the purified strains in step (2) and inoculate them on NA solid agar plates containing 1% soluble starch, and culture them upside down at a constant temperature of 60°C to 65°C overnight;

[0027] (4) Those with starch decomposition circles in iodine solution color development are named CHB1.

[0028] 2 Identification of strain CHB1

[0029] The growth temperature range of the bacteria is 47°C-75°C, and the optimum growth temperature is 60°C-65°C; the colony is round, light yellow and transparent,...

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Abstract

The present invention relates to a Geobacillus caldoxylosilyticus strain producing cyclodextrin glycosyltransferase, wherein the strain is Geobacillus caldoxylosilyticus CHB1, is preserved in China Center for Type Culture Collection on August 28, 2013, and has the preservation number of CCTCC M2013384, and it is proved that the enzyme produced by the Geobacillus caldoxylosilyticus is cyclodextrin glycosyltransferase (short for CGTase) belonging to the alpha-amylase family, and can be adopted to catalyze soluble starch so as to generate alpha-cyclodextrin. According to the present invention, the new strain Geobacillussp CHB1 producing CGTase is provided, the optimum enzyme activity reaction temperature of the enzyme production fermentation broth supernatant of the strain is 65 DEG C, the activity is maintained between 40-85 DEG C, and the CGTase produced by the strain can be used for producing alpha-cyclodextrin, and can further be used for processing processes of starch in the temperature range and the like.

Description

technical field [0001] The present invention relates to a Geobacillus stearothermophilus and its application, especially the discovery of a new high-temperature cyclodextrin glucosyltransferase (CGTase) strain Geobacillus sp and the use of the strain to ferment and produce cyclodextrin glucosyltransferase An enzyme belongs to the field of fermentation production. Background technique [0002] Cyclodextrin glucosyltransferase is a class of amylases that can catalyze the conversion of starch into cyclodextrins. It is an important member of the α-amylase family and has four activities: cyclization, disproportionation, coupling and hydrolysis. In production, it is mainly used in the cyclization reaction to generate cyclodextrin, and the catalyzed products of cyclodextrin glucosyltransferase are α, β and γ three cyclodextrins, which are respectively composed of 6, 7 and 8 glucose molecules with α -1.4 glycosidic bonds connected end to end. According to different main products, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N9/10C12R1/01
Inventor 林新坚陈济琛贾宪波林戎斌林陈强
Owner INST OF SOIL & FERTILIZER FUJIAN ACADEMY OF AGRI SCI
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