Method for preparing L-tertiary leucine compound by two enzyme system

A technology for tert-leucine and compounds, which is applied in the field of preparation of L-tert-leucine compounds by a dual-enzyme system, can solve problems such as expensive coenzymes, difficult storage and recovery, and easy inactivation, so as to reduce production costs and operate The effect of simplicity, good technology application and industrialization prospects

Inactive Publication Date: 2010-09-29
ZHEJIANG UNIV
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Problems solved by technology

However, the coenzyme used is more expensive, and it is not easy to store and recycle, and it is relatively easy to inactivate

Method used

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  • Method for preparing L-tertiary leucine compound by two enzyme system
  • Method for preparing L-tertiary leucine compound by two enzyme system
  • Method for preparing L-tertiary leucine compound by two enzyme system

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Abstract

The invention relates to a method for preparing a chiral compound based on splitting by adopting a biological enzyme preparation, in particular to a method for preparing an L-tertiary leucine compound by a two enzyme system. The method prepares the chiral L-tertiary leucine compound of the formula III by using the reaction of an N-acylating tertiary leucine compound of the formula II in water or a buffer solvent at 15 to 60 DEG C in the presence of the two enzyme system. The biological enzyme is a mixed enzyme of an acylating amino acid racemase (alanine racemase) and a hydrolase; and the preparation reaction equation is as follows, wherein X is any group of OH, NH2, NR1R2 and OR3 (wherein R1, R2, R3 are a C1-C5 straight chain, a branch chain and a one, two or three-halogen substituted alkane respectively). The invention can be implemented at room temperature and ensures simple operation and no pollution, while remarkably lowering the production cost.

Description

technical field The invention relates to a method for preparing chiral compounds based on the resolution of biological enzyme preparations, in particular to a method for preparing L-tert-leucine compounds by a dual-enzyme system. Background technique L-tert-leucine (III) is mainly used as nutritional enhancer, animal feed additive, and synthetic drug. As a synthetic drug intermediate, it is mainly used to synthesize anti-AIDS (HIV) drugs Atazanavir and Sandoz, as well as some anticancer drugs, biological inhibitors and biologically active peptides. The anti-HIV protease inhibitor atazanavir is currently the main anti-AIDS drug in the world. In the United States alone, about 130,000 patients have been treated with this drug. The active ingredient of the drug-"C-terminal "Intermediate" is an important intermediate for the synthesis of the powerful protease inhibitor atazanavir, which has the characteristics of sustained and strong inhibition of HIV, low drug resistance, co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P41/00C12P13/02
Inventor 于洪巍王博车大庆沈文和
Owner ZHEJIANG UNIV
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