Cryoprotectant and application thereof in cryopreservation of umbilical cord mesenchymal stem cells

Abstract

The invention discloses a cryoprotectant. The cryoprotectant comprises the following components: dimethyl sulfoxide in volume percentage of 1-5%, 20-80mmol/L trehalose, 5-15ug/mL catalase, 10-40ug/mLZ-valine-alanine racemase-ASP (methyl ester)-methyl fluoride ketone, 0.1-3ug/mL insulin and 0.01-10mg/L selenium. The cryoprotectant disclosed by the invention is serum-free, so that the pollution ofpathogenic bacteria can be avoided; the content of dimethyl sulfoxide (DMSO) is less, so that the cytotoxicity is reduced; the cryoprotectant disclosed by the invention is prepared from pure chemicalsubstances, the components are stable and the batch-to-batch stability is high; after the cells cryopreserved with the cryoprotectant disclosed by the invention revive, the cell viability is obviouslyenhanced. The invention also discloses an application of the cryoprotectant in cryopreservation of umbilical cord mesenchymal stem cells. The invention also discloses a cryopreservation method of theumbilical cord mesenchymal stem cells.

Description

technical field [0001] The invention relates to a cryopreservation solution and its application, in particular to a cryopreservation solution and its application in cryopreservation of umbilical cord mesenchymal stem cells. Background technique [0002] Mesenchymal stem cells are important members of the stem cell family, which can be induced under specific in vivo or in vitro conditions. It can be differentiated into fat, bone, cartilage, muscle, tendon, ligament, nerve, liver, myocardium, endothelium and other tissue cells for clinical treatment of various diseases, such as nervous system diseases, kidney diseases, autoimmune diseases, malignant tumors, etc. Provides new avenues. Mesenchymal stem cells mainly include bone marrow mesenchymal stem cells, fat mesenchymal stem cells and umbilical cord mesenchymal stem cells. At present, bone marrow mesenchymal stem cells are the most widely studied. The number, expansion and differentiation of bone marrow mesenchymal stem ce...

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Problems solved by technology

At present, the preservation of umbilical cord mesenchymal stem cells is mostly the cryopreserv...

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