Detection reagent for alanine aminotransferase

A technology of alanine amino group and detection reagent, which is applied in the field of medical inspection, can solve the problems of lack of inspection professionals and insufficient automatic biochemical inspection equipment and facilities, and achieves the effects of good sensitivity, low price and easy operation.

Active Publication Date: 2013-09-25
NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] With the "Eleventh Five-Year" plan for the purchase and strengthening of basic equipment for tertiary and secondary hospitals, hospitals above the middle level have good hardware and software facilities, but grassroots medical institutions, especially health centers, clinics and other units are not fully automatic. Biochemical testing equipment and facilities are generally insufficient, and there is not a sufficient number of testing professionals. Therefore, the establishment of a POCT analysis system that is easy to operate, low in price, and real-time reporting is of great significance to the role of grassroots hospitals in disease prevention and diagnosis and treatment.

Method used

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  • Detection reagent for alanine aminotransferase
  • Detection reagent for alanine aminotransferase
  • Detection reagent for alanine aminotransferase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Diluent:

[0047] Tris hydroxymethyl amino buffer (pH7.0) 0.1mol / L

[0048] TWEEN805%

[0049] Proclin 3000.1%

[0050] Bilirubin oxidase 1KU / L

[0051] Vitamin C oxidase 1KU / L

[0052] Reagent:

[0053] Tris hydroxymethyl amino buffer (pH6.0) 0.1mol / L

[0054] L-alanine 20mol / L

[0055] α-ketoglutarate 1mol / L

[0056] Phosphoric acid 20mol / L

[0057] Pyruvate oxidase 20KU / L

[0058] Thiamine pyrophosphate 10mol / L

[0059] Magnesium chloride 10mol / L

[0060] Peroxidase 10KU / L

[0061] 4-aminoantipyrine 1mol / L

[0062] Sodium N-ethyl-N-(3-sulfopropyl)-3-methoxyaniline 2mol / L

[0063] Potassium sorbate 0.1g / L

[0064] Bovine serum albumin 5g / L

Embodiment 2

[0066] Diluent:

[0067] Tris hydroxymethyl amino buffer (pH7.5) 0.5mol / L

[0068] TRITONX-1005%

[0069] Methylparaben 1%

[0070] Potassium ferrocyanide 1mol / L

[0071] Vitamin C oxidase 2KU / L

[0072] Reagent:

[0073] 3-(N-morpholine)sodium ethanesulfonate buffer solution (pH7.2) 0.5mol / L

[0074] L-alanine 20mol / L

[0075] α-ketoglutarate 5mol / L

[0076] Phosphoric acid 10mol / L

[0077] Pyruvate oxidase 50KU / L

[0078] Thiamine pyrophosphate 20mol / L

[0079] Magnesium chloride 10mol / L

[0080] Peroxidase 50KU / L

[0081] 4-aminoantipyrine 3mol / L

[0082] N-ethyl-N-(2-hydroxy-3-propanesulfonyl) m-toluidine 2mol / L

[0083] Proclin3001g / L

[0084] Trehalose 10g / L

Embodiment 3

[0086] Diluent:

[0087] 3-Morpholinepropanesulfonic acid buffer (pH6.5) 1.0mol / L

[0088] SPAN2010%

[0089] Sodium Benzoate 1%

[0090] Potassium ferricyanide 10mol / L

[0091] Vitamin C oxidase 10KU / L

[0092] Reagent:

[0093] Piperazine-N,N-bis(2-hydroxyethanesulfonic acid) buffer solution (pH8.0) 1mol / L

[0094] L-alanine 50mol / L

[0095] α-ketoglutarate 2mol / L

[0096] Phosphoric acid 50mol / L

[0097] Pyruvate oxidase 100KU / L

[0098] Thiamine pyrophosphate 50mol / L

[0099] Magnesium chloride 100mol / L

[0100] Peroxidase 100KU / L

[0101] 4-aminoantipyrine 5mol / L

[0102] N-ethyl-N-(3-sulfopropyl)-3-methoxyaniline sodium salt 5mol / L

[0103] Proclin3001g / L

[0104] Fatty alcohol polyoxyethylene ether 10g / L

[0105] The performance of the reagent obtained in Example 1 of the present invention is described below in conjunction with the table.

[0106] 1. Precision

[0107] Table 1. Precision evaluation results

[0108]

[0109] 2. Linear

[0110] The s...

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Abstract

The invention discloses a detection reagent for alanine aminotransferase. The detection reagent comprises a diluent and a reaction reagent. The diluent comprises a buffer, a surfactant, an antiseptic, a bilirubin-interference removing reagent, and an ascorbic oxidase. The reaction reagent comprises a buffer, L-alanine, alpha-ketoglutaric acid, phosphoric acid, pyruvate oxidase, thiamine pyrophosphate, magnesium chloride, peroxidase, 4-aminoantipyrene, a chromogen, an antiseptic, and a freeze-drying protecting agent. The detection reagent has advantages of good sensitivity, accuracy, precision and linearity, and can completely meet the requirements of clinical examination.

Description

technical field [0001] The invention relates to the technical field of medical testing, in particular to an alanine aminotransferase assay reagent for a POCT analyzer. Background technique [0002] Liver disease is a common and extremely harmful disease. Its timely diagnosis and treatment play an important role in the prevention, diagnosis and treatment of the disease. Alanine aminotransferase (ALT) mainly exists in blood and related tissues. The intracellular concentration is 1000-3000 times higher than the serum concentration. As long as 1% of the liver tissue is destroyed, the serum concentration can be doubled. It is recommended by the World Health Organization as the most sensitive detection index of liver function damage. The determination of ALT Active against toxic hepatitis, viral hepatitis, active cirrhosis, liver cancer, obstructive jaundice, fatty liver, cholangitis and cholecystitis, myocardial infarction, myocarditis, cardiovascular disease, skeletal muscle dis...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/52C12Q1/26
Inventor 邹炳德邹继华沃燕波张桂春周海滨
Owner NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD
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