Method for preparing alanyl-glutamine dipeptide through biological enzyme conversion

A technology of glutamate dipeptide and biological enzyme is applied in the field of preparation of glutamate dipeptide, which can solve the problems of high cost, long production cycle, large environmental pollution and the like, and achieve the effects of high catalytic conversion reaction efficiency, easy control of quality indicators and high catalytic conversion ability.

Inactive Publication Date: 2016-01-27
JING JING PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The preparation technology of CG dipeptide mainly includes chemical synthesis method and microbial enzyme conversion method. The chemical synthesis method mainly has the disadvantages of long synthesis route, long production cycle, large environmental pollution, strict process conditions and high cost.
[0004] The patent with the publication number CN104480075A discloses a method for preparing glutamic acid dipeptide by catalytic conversion of biological enzymes. The preparation and use of biological enzymes adopt the form of biological enzyme freeze-dried powder, which is inconvenient to use, and has low enzyme activity and low material reaction rate.

Method used

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  • Method for preparing alanyl-glutamine dipeptide through biological enzyme conversion
  • Method for preparing alanyl-glutamine dipeptide through biological enzyme conversion
  • Method for preparing alanyl-glutamine dipeptide through biological enzyme conversion

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Take 350g of biological enzyme and dissolve it in 3000ml of dipotassium hydrogen phosphate-phosphate buffer solution, adjust the pH to 7.5, and control the temperature at 5-10°C to prepare the biological enzyme solution. The detected enzyme activity is 5122U. Use purified water to prepare dilute hydrochloric acid and dilute ammonia water with a mass fraction of 15% respectively with hydrochloric acid and ammonia water for later use.

[0023] Take 5kg of the reaction substrate isopropyl alanine and 2.5kg of glutamine and dissolve it in 50L of purified water, slowly and evenly add the biological enzyme solution into the reaction substrate, control the addition rate at 15ml / min, and monitor the pH of the reaction solution in real time. Add the prepared dilute hydrochloric acid and dilute ammonia water to the reaction substrate, control the pH of the reaction solution at 5-10, and maintain the temperature at 15-25°C. Sampling detection in the reaction process, liquid chrom...

Embodiment 2-4

[0026]

[0027]

Embodiment 5-7

[0030]

[0031]

[0032] It should be noted that the dilute hydrochloric acid and dilute ammonia water used to regulate the pH in the reaction solution can be prepared in different concentrations according to the needs, which is convenient for use.

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Abstract

The invention relates to the technical field of the preparation for alanyl-glutamine dipeptide and particularly discloses a method for preparing alanyl-glutamine dipeptide through biological enzyme conversion. The method comprises the following steps: 1) preparing a biological enzyme liquid: dissolving the biological enzyme in a buffered liquid, controlling pH value within the scope of 7.0+ / -2.0, controlling the temperature at 5-10 DEG C, thereby acquiring the biological enzyme liquid, wherein the mass fraction of the ester acyl-containing transferring biological enzyme is 5%-20%; 2) dissolving the ester derivates of substrate L-alanine and glutamine in pure water, and then uniformly dropping the biological enzyme liquid into a reaction liquor, and meanwhile, adding acid liquor or alkali liquor, controlling the pH of the reaction liquor at 5-10, reacting under the temperature controlled at 15-25 DEG C, sampling and detecting the production of the alanyl-glutamine dipeptide during the reaction process, stopping reaction when the content of the alanyl-glutamine dipeptide is highest, and separating the acquired converted liquid, thereby acquiring the alanyl-glutamine dipeptide. According to the method provided by the invention, the enzyme activity is high, the reaction rate of the materials is higher, the method has the advantages of simple technique control, easy control on quality index and low cost, and the method is more fit for industrial production.

Description

technical field [0001] The invention relates to the technical field of preparation of glucodipeptide. Background technique [0002] N(2)-L-alanyl-L-glutamine (also known as alanyl glutamine) is a derivative of amino acid, referred to as proglycine dipeptide, which is mainly used as amino acid parenteral nutrition and has important medical value and economic value. [0003] The preparation technology of CG dipeptide mainly includes chemical synthesis method and microbial enzyme conversion method. The chemical synthesis method mainly has the disadvantages of long synthesis route, long production cycle, large environmental pollution, strict process conditions and high cost. [0004] The patent with the publication number CN104480075A discloses a method for preparing glucodipeptide by catalytic conversion of biological enzymes. The preparation and use of the biological enzymes adopt the form of biological enzyme freeze-dried powder, which is inconvenient to use, and has low enz...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/02
Inventor 李斌水何连顺
Owner JING JING PHARMA
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