Paenibacillus polymyxa pesticide-fertilizer for farm onsite fermentation and applications thereof

Abstract

The invention relates to a paenibacillus polymyxa pesticide-fertilizer for farm onsite fermentation, a preparation method, and applications thereof. The pesticide-fertilizer comprises the following raw materials: paenibacillus polymyxa powder, colistin sulfate, natamycin, L-alanine, valine, yeast powder, corn steep liquor powder, disodium hydrogen phosphate, sodium chloride, fructose, and brown sugar. The colistin sulfate and natamycin in the insecticide-fertilizer can inhibit the growth of fungi (such as pathogenic fungi, yeast, etc.) and gram negative bacteria (such as colibacillus, salmonella, etc.) during the fermentation process. The spore germinator (alanine, fructose, and valine) can rapidly promote the germination of spores of paenibacillus polymyxa and improve the germination rate of spores. The provided pesticide-fertilizer can be applied to the onsite fermentation and propagation in a simple and crude farm. The required equipment is simple. The using amount of bacterium is little. The cost is low. The fermentation broth obtained through onsite fermentation has the advantages of high content and good activity. The insecticide-fertilizer can prevent disease, promote growth, and increase output and improve product quality, and can be used as a microbial pesticide, and the effect is stable.

Description

technical field [0001] The invention relates to the field of microbial fertilizers and microbial pesticides, in particular to a Paenibacillus polymyxa medicinal fertilizer applied to on-site fermentation in farms and its application. Background technique [0002] With the development of existing agricultural policies, the trend of farm scale and large-scale is further strengthened. Bacillus is currently the most studied microorganism in biological pesticides and biological fertilizers. It has broad-spectrum antibacterial activity and strong stress resistance. , and its vegetative cells can reproduce and colonize on the surface of fungal pathogenic mycelium, effectively inhibiting the growth of pathogens. Therefore, it has shown broad application prospects in controlling disease occurrence, promoting plant growth, and increasing crop yield. [0003] Paenibacillus polymyxa is a spore-forming, Gram-positive bacterium belonging to the genus Paenibacillus. Its colonies are mostl...

AI Technical Summary

Problems solved by technology

[0009] Second, it takes a long time in the early stage of spore germination, usually 4 hours before the spores will start to germinate. At this time, due to the large number of yeast and lactic acid bacteria in the "activation" solution, the pH drops rapidly, which can already inhibit the germination of spores. reproduction, thereby greatly affecting spore germination;

[0010] 3. It is also easy to infect Escherichia coli in high-nutrient medium, the original plant pathogenic bacteria and other harmful bacteria, resulting in poor effect after application and still have adverse effects;

[0011] Fourth, the amount of spores is large, and the effect is unstable

[0012] With the permission of land transfer, the country advocates farm-style management, and the trend of large-scale farms will be further strengthened. The expansion and fermentation of bio-control bacteria on the farm will meet the needs of the agricultural market. However, it has not been disclosed so far. A kind of Paenibacillus polymyxa fertilizer that can better control the miscellaneous bacteria and promote the rapid germination of spores and its application related technology is applied to the on-site fermentation and propagation of the farm.

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