Bacillus amyloliquefaciens insecticide-fertilizer for farm onsite fermentation and applications thereof

Abstract

The invention relates to a bacillus amyloliquefaciens insecticide-fertilizer for farm onsite fermentation, a preparation method and applications thereof. The insecticide-fertilizer comprises the following raw materials: bacillus amyloliquefaciens, colistin sulfate, natamycin, L-alanine, asparagine, yeast extract powder, peptone, disodium hydrogen phosphate, sodium chloride, glucose, fructose, and brown sugar. The colistin sulfate and natamycin in the insecticide-fertilizer can inhibit the growth of fungi (such as pathogenic fungi, yeast, etc.) and gram negative bacteria (such as colibacillus, salmonella, etc.) during the fermentation process. The spore germinator (alanine, fructose, and asparagine) can rapidly promote the germination of spores of bacillus amyloliquefaciens and improve the germination rate of spores. The provided insecticide-fertilizer can be applied to the onsite fermentation and propagation in a simple and crude farm. The required equipment is simple. The using amount of bacterium is little. The cost is low. The fermentation broth obtained through onsite fermentation has the advantages of high content and good activity. The insecticide-fertilizer can prevent disease, promote growth, and increase output and improve quality, and can be used as a microbial pesticide, and the effect is stable.

Description

technical field [0001] The invention relates to the field of microbial fertilizers and microbial pesticides, in particular to a bacillus amyloliquefaciens medicinal fertilizer applied to on-site fermentation in farms and its application. Background technique [0002] With the development of existing agricultural policies, the trend of farm scale and large-scale is further strengthened. Bacillus is currently the most studied microorganism in biological pesticides and biological fertilizers. It has broad-spectrum antibacterial activity and strong stress resistance. , and its vegetative cells can reproduce and colonize on the surface of fungal pathogenic mycelium, effectively inhibiting the growth of pathogens. Therefore, it has shown broad application prospects in controlling disease occurrence, promoting plant growth, and increasing crop yield. [0003] Bacillus amyloliquefaciens has a wide antibacterial spectrum, rich metabolites, and can produce a variety of antibacterial s...

AI Technical Summary

Problems solved by technology

[0008] The second is that the early stage of spore germination takes a long time, usually 4 hours before the spores will start to germinate. At this time, due to the large-scale reproduction of yeast and lactic acid bacteria in the "activation" solution, the pH drops rapidly, which can already inhibit the germination and reproduction of spores. , thereby greatly affecting the germination of spores;

[0009] The third is that the high-nutrient medium is also susceptible to infection of Escherichia coli, the original plant pathogenic bacteria and other harmful bacteria, resulting in poor effects after application and still have adverse effects;

[0010] Fourth, the amount of spores used is large, and the effect is unstable

[0011] However, there has not been disclosed so far a related technology related to a Bacillus amyloliquefaciens fertilizer that can better control miscellaneous bacteria and promote rapid germination of spores and its application when applied to on-site fermentation and propagation of farms.

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