Calf serum 'double resistant' culture medium

Abstract

The invention discloses a liquid culture medium, which is allocated by calf serum, glucose peptone water, vancocin and polymyxin B to prevent non-target bacterial fertility.

Description

technical field [0001] The invention relates to a culture medium applied to the detection of "pathogenic bacteria (Neisseria meningitidis)" in the aspect of "microorganism detection" in "medical microbiology". Background technique [0002] Neisseria meningitidis has very high requirements on environmental factors. After leaving the human body, it is very easy to die when the environment is not suitable (such as death within 2 hours if the temperature is lower than 22 ° C), but the epidemic encephalopathy caused by Neisseria meningitidis The popular season of spinal meninges is winter and spring, so the investigation of the infection has to be carried out in the cold winter and spring. Before the invention of this technology, the detection of Neisseria meningitidis was to collect throat swabs of patients, suspected patients or carriers, and directly inoculate the throat swabs on blood agar plates or chocolate-colored blood agar plates. There are also very few people who firs...

AI Technical Summary

Problems solved by technology

There are some disadvantages in these mediums: one is that the preparation of "yolk double-antibody saline" is very cumbersome, and requires 75% alcohol to soak and sterilize eggs, remove egg whites, collect egg yolks, and break them into pieces. The second is that a large amount of plate culture medium needs to be transported and heat preservation equipment is required when sampling at the site. The glass plate will not only be damaged during transportation, but also cause contamination to the sample, which is very inconvenient for the work; the third is the above The culture medium must be cultivated immediately after the sample is inoculated. When the laboratory is far away from the sampling site, it is inconvenient to cultivate. At this time, the bacteria in the sample will gradually die, which reduces the possibility of detecting the target bacteria in the future; the fourth is the above-mentioned Plate medium is a solid medium, they only produce a single colony when cultivating bacteria, and they do not have a bacterial enrichment effect, so when the sample contains a small amount of bacteria, it cannot be detected