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Bacillus megaterium bacterial fertilizer for onsite fermentation in farm and applications thereof

A technology of Bacillus megaterium and bacterial fertilizer, applied in the field of microbial fertilizers, can solve the problems of being susceptible to Escherichia coli, affecting the germination of spores, and taking a long time, and achieving the effect of simple facility requirements, easy implementation, and less bacterial consumption

Inactive Publication Date: 2016-03-30
FOSHAN YANHUI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The second is that the early stage of spore germination takes a long time, usually 4 hours before the spores will start to germinate. At this time, due to the large-scale reproduction of yeast and lactic acid bacteria in the "activation" solution, the pH drops rapidly, which can already inhibit the germination and reproduction of spores. , thereby greatly affecting the germination of spores;
[0007] The third is that the high-nutrient medium is also susceptible to infection of Escherichia coli, the original plant pathogenic bacteria and other harmful bacteria, resulting in poor effects after application and still have side effects
[0008] However, there has not been disclosed so far about a Bacillus megaterium bacterial fertilizer that can better control miscellaneous bacteria and promote rapid germination of spores, which is applied to on-site fermentation and propagation of farms.

Method used

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  • Bacillus megaterium bacterial fertilizer for onsite fermentation in farm and applications thereof
  • Bacillus megaterium bacterial fertilizer for onsite fermentation in farm and applications thereof
  • Bacillus megaterium bacterial fertilizer for onsite fermentation in farm and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] A kind of Bacillus megaterium bacterial fertilizer used for on-site fermentation on the farm, the raw materials for preparing the Bacillus megaterium bacterial fertilizer are: 100 parts of Bacillus megaterium bacterial powder, 18 parts of colistin sulfate, 1 part of natamycin, 1 part of inosine, 18 parts of lysine, 157 parts of corn flour, 120 parts of soybean meal powder, 3 parts of disodium hydrogen phosphate, 2 parts of potassium chloride, 80 parts of brown sugar;

[0041] Wherein, the preparation method of the Bacillus megaterium bacterial fertilizer for on-site fermentation of the farm comprises the following steps:

[0042] , Preparation of Bacillus megaterium powder

[0043] Take out the preservation tube of Bacillus megaterium, scratch the plate with LB medium for recovery, and culture at 30°C for 2-3 days. Pick larger colonies on the plate and inoculate them into eggplant bottles filled with LB medium, and culture them in an incubator at 30°C for 2-3 days. ...

Embodiment 2

[0050] The fermented liquid of the bacillus megaterium bacterium fertilizer cultivated in the field of embodiment 1 is tested on strawberry (hereinafter referred to as the bacillus megaterium bacterium liquid)

[0051]A. Test location: a grower in Taohong Town, Longhui County, Hunan Province

[0052] B. Test method: Test group: During the planting period, the seedlings were planted, and the roots were irrigated once with an 800-fold dilution of Bacillus megaterium bacteria solution. During the growth period of strawberries, the leaves were sprayed with 1:300 times Bacillus megaterium bacteria solution for 10 days (day) 1 time, continuous spraying 3 times, no phosphate fertilizer was applied; control group: apply water and test group at the same time, and fertilize as usual;

[0053] C. test results:

[0054] It was found that early flowering and fruiting, large and plump fruits, many fruits, few abnormal-shaped fruits, good taste, and bright coloring, the average yield per...

Embodiment 3

[0056] A kind of Bacillus megaterium bacterial fertilizer used for on-site fermentation on the farm, the raw materials for preparing the Bacillus megaterium bacterial fertilizer are: 100 parts of Bacillus megaterium bacterial powder, 14 parts of colistin sulfate, 10 parts of natamycin, 0.8 parts of inosine, 16 parts of lysine, 160 parts of corn flour, 120 parts of soybean meal powder, 4 parts of disodium hydrogen phosphate, 1 part of potassium chloride, 80 parts of brown sugar;

[0057] Wherein, the preparation method of the Bacillus megaterium bacterial fertilizer for on-site fermentation of the farm comprises the following steps:

[0058] , Preparation of Bacillus megaterium powder

[0059] Take out the preservation tube of Bacillus megaterium, scratch the plate with LB medium for recovery, and culture at 30°C for 2-3 days. Pick larger colonies on the plate and inoculate them into eggplant bottles filled with LB medium, and culture them in an incubator at 30°C for 2-3 day...

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Abstract

The invention relates to a bacillus megaterium bacterial fertilizer for onsite fermentation in a farm and applications thereof. The bacterial fertilizer comprises the following raw materials: bacillus megaterium powder, colistin sulfate, natamycin, inosine, lysine, corn flour, soybean cake powder, disodium hydrogen phosphate, potassium chloride, and brown sugar. The bacillus megaterium bacterial fertilizer can be applied to onsite fermentation and propagation in a farm. The colistin sulfate and natamycin in the bacterial fertilizer can inhibit the growth of fungi (such as pathogenic fungi, yeast, etc.) and gram negative bacteria (such as colibacillus, salmonella, etc.) during the fermentation process. The spore germinator (inosine and lysine) can rapidly promote the germination of spores of bacillus megaterium and improve the germination rate of spores. The provided preparation can be applied to the onsite fermentation and propagation in a simple and crude farm. The required equipment is simple. The using amount of bacterium is little. The cost is low. The fermentation broth obtained through onsite fermentation has the advantages of high content, good activity and few contaminating microorganisms. The bacterial fertilizer can decompose phosphorus, prevent disease, promote growth, increase output, and improve product quality. The effect is stable.

Description

technical field [0001] The invention relates to the field of microbial fertilizers, in particular to a Bacillus megaterium bacterial fertilizer applied to on-site fermentation in farms and its application. Background technique [0002] The average content of phosphorus in the earth's crust is about 0.122%. During the process of soil formation, phosphorus migrates to a certain extent. With the progress of soil formation, the content of phosphorus in the soil also decreases. The phosphorus content of most soils in my country is between 0.043% and 0.046%, so the phosphorus content in the soil is relatively deficient. Since phosphorus is one of the 16 nutrient elements necessary for plant growth and development, crops mainly absorb phosphorus in chemical forms such as phosphate, while phosphorus in soil mainly exists in the state of fixed minerals, accounting for about 70% of the total phosphorus content. %, so the content of water-soluble inorganic phosphate ions in the soil p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/00C05F17/00A01C21/00C12N1/20C12R1/11
CPCA01C21/00C05B7/00C05F17/00C05G3/00C05G5/23C12N1/20C05C11/00C05D1/02C05F5/002C05F11/00C05F11/08Y02W30/40
Inventor 胡艳晖其他发明人请求不公开姓名
Owner FOSHAN YANHUI BIOTECH CO LTD
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