Method for preparing viable bacillus subtilis preparation for feed

Abstract

The invention discloses a method for preparing a viable bacillus subtilis preparation for feed. The viable bacillus subtilis preparation for the feed is prepared by the following steps of: (1) performing activation culture on bacillus subtilis strains; (2) mutagenizing and screening the activated bacillus subtilis strains; (3) performing propagation culture on the screened bacillus subtilis strains; (4) preparing bacillus subtilis liquid from the bacillus subtilis strains subjected to the propagation culture; (5) mixing the bacillus subtilis liquid and an absorption carrier, wherein the absorption carrier is a mixture of maize alcohol lees and maize cob meal; and (6) pneumatically drying the bacillus subtilis liquid-absorbed carrier to obtain the finished product. By the method, the low-cost and stable-quality viable bacillus subtilis preparation can be prepared.

Description

technical field [0001] The invention relates to a production method of a live bacterial preparation, in particular to a production method of a live Bacillus subtilis preparation for feed. Background technique [0002] Bacillus subtilis can inhibit harmful bacteria such as Escherichia coli and Staphylococcus aureus when it enters the animal body, consumes oxygen in the intestine, degrades macromolecules, and is beneficial to the growth of intestinal anaerobic bacteria, especially lactic acid bacteria. At the same time, it can produce protease, fiber It is beneficial to the digestion and absorption of food by animals. There are mainly two types of Bacillus subtilis preparations in the prior art, one is the powder prepared by liquid fermentation spray drying, the other is the powder obtained by crushing after solid state fermentation, and the liquid fermentation spray drying method is used to produce Bacillus subtilis The high cost of the preparation is not conducive to large-...

AI Technical Summary

Problems solved by technology

There are mainly two types of Bacillus subtilis preparations in the prior art, one is the powder prepared by liquid fermentation spray drying, the other is the powder obtained by crushing after solid state fermentation, and the liquid fermentation spray drying method is used to produce Bacillus subtilis The cost of the preparation is high, which is not conducive to large-scale promotion and use in feed; and the Bacillus subtilis preparation produced by the solid-state fermentation method is easily infected by miscellaneous bacteria during the fermentation process, which affects the quality of the Bacillus subtilis preparation