44results about How to "Bacteria content increased" patented technology

The invention relates to a method for cleanly and efficiently producing a microorganism bactericide, and the method comprises the following steps: adding adsorptive carriers in a liquid yeast culture medium, such as one or more of active carbon, zeolite, medical stone, sepiolite, active soil, diatomite, montmorillonite, attapulgite or all other artificial porous adsorptive materials, carrying outhigh-temperature sterilization together with a culture medium, cooling at a proper temperature, inserting bacteria, fermenting according to proper bacteria breeding conditions, wherein viable bacteria and metabolic product are adsorbed on the carriers after the fermentation is completed; and quickly settling after standing, and collecting a settlement, and drying or drying after pelletizing the settlement. A supernatant fluid after the fermentation product is collected and can be used as recycled water after being subjected to sterilization, or be used for preparing a culture medium for recycling.

This invention relates to a method to prepare a kind of bioorganic fertilizer and its application. The preparation method includes following steps: a) proliferation and cultivation of microorganism species; b) cultivation of microorganism species against stalk antibody; c) adsorption onto a carrier and drying into organic decomposers; d) preprocessing of organic waste; e) inoculation complex fermentation and f) continuous high-temperature fermentation and nontoxification. This invention has the advantages of advanced technique, low production cost, stable product quality and long fertility persistence and can be widely applied in the production of corns, vegetables, fruits and all kinds of economic crops. Besides, it is a green method makes considerable economic, social and environmental benefits.

The invention relates to a straw decomposing agent produced from raw materials of bacteria and fungi, and a production method thereof. Bacillus subtilis, saccharomyces cerevisiae, Aspergillus oryzae and aspergillus niger are assembled as composite bacteria and are individually cultured through steps of preparation of medium, control of an inoculation amount, a fermentation temperature and a fermentation time, and adjusting of a PH value parameter, concentration and drying. The bacteria are blended and proportioned to prepare into a decomposing agent capable of returning crop straws into field on the spot. The decomposing agent has advantages of strong pertinence, good decomposing effect and stable quality and performance.

The invention discloses an active lactobacillus casei beverage and a preparation method thereof and relates to an active probiotics beverage in the technical field of microorganism fermentation. The beverage comprises the following components in percentage by mass: 25 to 40 percent of fermented milk, 8 to 12 percent of white granulated sugar, 0.3 to 0.5 percent of stabilizing agent, 0.03 to 0.05 percent of food flavor and 50 to 70 percent of purified water. The preparation method comprises the following steps of: 1, preparing base materials; 2, fermenting the base materials; (3) preparing and disinfecting sugar liquor; and (4) preparing the beverage. Compared with the prior art, the method has the advantages of remarkably shortened product fermentation time, remarkably increased bacteria content of lactobacillus casei, lowered product cost and fermentation pollution risk, lowered equipment investment and suitability for producing the lactobacillus casei probiotics beverage under the conventional equipment condition by national dairy enterprises.

The invention discloses a water quality improver for aquaculture. The water quality improver is prepared from the following components in percentage by mass: 30 percent to 40 percent of a microorganism strain, 20 percent to 30 percent of a plant mixture, 5 percent to 10 percent of a biological growth accelerant, 2 percent to 5 percent of an amino acid, 0.1 percent to 0.5 percent of chitosan and the balance of water; the microorganism strain disclosed by the invention is prepared from the following components: rhodopseudomonas palustris, bacillus subtilis, denitrifying bacteria, bacillus licheniformis, candida utilis, enterococcus faecalis, enterococcus faecium, bacillus laterosporus, denitrifying bacteria, photosynthetic bacteria and lactic acid bacteria; the water quality improver disclosed by the invention has a long acting time and is convenient to use; when the water quality improver is used for adjusting the water quality, the water quality improver also can be used for accelerating photosynthesis of alga to generate oxygen for alleviating a hypoxia phenomenon of fishes. The water quality improver has the advantages of high bacterium content, strong activity and small utilization dosage.

The invention relates to a bacillus amyloliquefaciens insecticide-fertilizer for farm onsite fermentation, a preparation method and applications thereof. The insecticide-fertilizer comprises the following raw materials: bacillus amyloliquefaciens, colistin sulfate, natamycin, L-alanine, asparagine, yeast extract powder, peptone, disodium hydrogen phosphate, sodium chloride, glucose, fructose, and brown sugar. The colistin sulfate and natamycin in the insecticide-fertilizer can inhibit the growth of fungi (such as pathogenic fungi, yeast, etc.) and gram negative bacteria (such as colibacillus, salmonella, etc.) during the fermentation process. The spore germinator (alanine, fructose, and asparagine) can rapidly promote the germination of spores of bacillus amyloliquefaciens and improve the germination rate of spores. The provided insecticide-fertilizer can be applied to the onsite fermentation and propagation in a simple and crude farm. The required equipment is simple. The using amount of bacterium is little. The cost is low. The fermentation broth obtained through onsite fermentation has the advantages of high content and good activity. The insecticide-fertilizer can prevent disease, promote growth, and increase output and improve quality, and can be used as a microbial pesticide, and the effect is stable.

The invention relates to a paenibacillus polymyxa pesticide-fertilizer for farm onsite fermentation, a preparation method, and applications thereof. The pesticide-fertilizer comprises the following raw materials: paenibacillus polymyxa powder, colistin sulfate, natamycin, L-alanine, valine, yeast powder, corn steep liquor powder, disodium hydrogen phosphate, sodium chloride, fructose, and brown sugar. The colistin sulfate and natamycin in the insecticide-fertilizer can inhibit the growth of fungi (such as pathogenic fungi, yeast, etc.) and gram negative bacteria (such as colibacillus, salmonella, etc.) during the fermentation process. The spore germinator (alanine, fructose, and valine) can rapidly promote the germination of spores of paenibacillus polymyxa and improve the germination rate of spores. The provided pesticide-fertilizer can be applied to the onsite fermentation and propagation in a simple and crude farm. The required equipment is simple. The using amount of bacterium is little. The cost is low. The fermentation broth obtained through onsite fermentation has the advantages of high content and good activity. The insecticide-fertilizer can prevent disease, promote growth, and increase output and improve product quality, and can be used as a microbial pesticide, and the effect is stable.

The invention discloses lactobacillus acidophilus fermented milk and a preparation method thereof, and relates to the lactobacillus acidophilus fermented milk in the technical fields of microbial fermentation and food processing. Firstly, the lactobacillus acidophilus fermented milk mainly comprises the following components by mass percent: 64-92% of raw milk, 3-6% of white granulated sugar, 4-8% of high fructose corn syrup, 0.1-20% of fruit juice and / or confiture, 0.1-0.3% of buffer salt, 0.1-1.0% of trehalose and 0.1-1.0% of bioactive peptide; secondly, the lactobacillus acidophilus fermented milk is fermented by using a single strain of lactobacillus acidophilus; the inoculation quantity is 1*10<6> to 1*10<7> CFU / g; thirdly, a proper thickener is added. The method comprises the following steps: (1) burdening for the product; (2) homogeneous sterilization; (3) product fermentation; (4) stirring or turning over a jar to cool; (5) bulking the product and cooking after refrigerating. Compared with the prior art, the bacteria content of the lactobacillus acidophilus disclosed by the invention is obviously improved; the fermentation time of the product is shortened; the product is exquisite and fresh in taste, and good in flavor.

The present invention relates to a method for producing a probiotic agent of live Lactobacillus bulgaricus, belonging to the field of microbial technology and specially relates to a liquid anaerobic fermentation method for producing the probiotic agent of live Lactobacillus bulgaricus. The method comprises the steps of: employing the Lactobacillus bulgaricus as a fermentation strain which is inoculated on an optimized liquid fermentation medium, fermenting at a temperature of 37-42 DEG C for 12-14h, drawing off and determining the number of viable cells, adsorbing fermentation broth by using vegetable starch, drying, crushing, and packaging to prepare the probiotic agent of live Lactobacillus bulgaricus with stable viability. The method of the invention is advantageous in that: high density fermentation of the Lactobacillus bulgaricus can be completed by using an ordinary fermentation tank, with simple operation, stable effect and low energy consumption; and centrifuges, freeze-drying machines, etc. are not required, thus reducing production costs and improving production efficiency.

The invention discloses a yogurt fermentative lactic bacteria combination and a fermenting agent, belonging to the technical field of fermentative milk product preparation. The yogurt fermentative lactic bacteria combination consists of streptococcus thermophilus and lactobacillus bulgaricus; the streptococcus thermophil comprises at least two different strains; the production properties of the two different strains are complementary to each other, and the production property is the acid production capability, the viscosity production capability and the antiphagin capability; or the streptococcus thermophilus comprises at least two different strains, the acid production capability and the viscosity production capability of the two different strains are complementary to each other, and the antiphagin mode of different strains is different. According to the fermenting agent prepared by adopting the yogurt fermentative lactic bacteria combination, the streptococcus thermophilus strains with different performance advantages are compounded, the production property is good and stable, the fermenting time is shortened, the bacteriophage pollution in the production environment is alleviated, the practicability is strong, and the prepared fermented product is good in taste, viscosity and flavor and high in quality.

The invention provides a microbial agent and a preparing method thereof. The microbial agent is prepared from jelly type bacillus, bacillus subtilis and bacillus licheniformis. In the microbial agent, the viable count of jelly type bacillus ranges from 1.8 billon cfu / g to 2.2 billon cfu / g, the viable count of bacillus subtilis ranges from 1.7 billon cfu / g to 1.9 billon cfu / g, and the viable count of bacillus licheniformis ranges from 1.2 billon cfu / g to 1.3 billon cfu / g. The preparing method of the microbial agent comprises the steps of strain selection, strain activation and expanding culture, strain fermentation and finished product obtaining. The microbial agent is high in strain content, low in infectious microbe rate and capable of effectively improving soil properties.

The invention relates to a production method for a streptococcus thermophilus viable bacteria preparation, belonging to the field of microbial technology and particularly relating to a liquid anaerobic fermentation production method for a streptococcus thermophilus micro-ecological preparation. The production method comprises the following steps of taking the streptococcus thermophilus as the fermented strain, inoculating into an optimized liquid medium, cultivating for 16-20 hours at 40-42 DEG C, putting the fermentation liquid in a tank and measuring the viable bacteria quantity, adsorbing the fermentation liquid through vegetable starch and drying, after treatment by polyethylene glycol (PEG) 4000 and sodium polyacrylate, drying, smashing and packaging, and preparing the streptococcus thermophilus preparation with stable activity. The production method has the advantages of completing high density fermentation of the streptococcus thermophilus through a common fermentation tank, being simple in operation, having stable effect and low energy consumption, using no equipment such as a centrifugal machine and a freeze dryer and the like, lowering the production cost, and improving the production efficiency.

A microbial process for treating the emulsified sewage containing oil features that a combined flora obtained through screening, inoculation and decontamination is added to the said sewage and the sewage flows through regulatable aerating pool, slane oil-removing pool and biologic aerating filter pool. Its advantages are short time (4-6 hr), and high removal rate of 98% for oil, or 95% for CODcr,or 95% for BOD5, so completely reaching the national standard for discharge.

The invention relates to a production method of a Pediococcus acidilactici viable preparation, particularly a production method of a Pediococcus acidilactici viable preparation by liquid anaerobic fermentation, belonging to the technical field of microorganisms. The method comprises the following steps: inoculating Pediococcus acidilactici used as a zymophyte strain into an optimized liquid nutrient medium, culturing at 37-40 DEG C for 16-18 hours, putting in a tank, determining the viable count, adsorbing the fermentation liquid with plant starch, drying, pulverizing, and packaging to obtain the stable-activity Pediococcus acidilactici preparation. The invention can implement high-density fermentation of Pediococcus acidilactici in a common fermentation tank, is simple to operate, has the advantages of stable effect and low energy consumption, does not use any centrifugal machine, freeze drier or the like, lowers the production cost, enhances the production efficiency, and thus, is easy to popularize and use.

The invention relates to a bacillus megaterium bacterial fertilizer for onsite fermentation in a farm and applications thereof. The bacterial fertilizer comprises the following raw materials: bacillus megaterium powder, colistin sulfate, natamycin, inosine, lysine, corn flour, soybean cake powder, disodium hydrogen phosphate, potassium chloride, and brown sugar. The bacillus megaterium bacterial fertilizer can be applied to onsite fermentation and propagation in a farm. The colistin sulfate and natamycin in the bacterial fertilizer can inhibit the growth of fungi (such as pathogenic fungi, yeast, etc.) and gram negative bacteria (such as colibacillus, salmonella, etc.) during the fermentation process. The spore germinator (inosine and lysine) can rapidly promote the germination of spores of bacillus megaterium and improve the germination rate of spores. The provided preparation can be applied to the onsite fermentation and propagation in a simple and crude farm. The required equipment is simple. The using amount of bacterium is little. The cost is low. The fermentation broth obtained through onsite fermentation has the advantages of high content, good activity and few contaminating microorganisms. The bacterial fertilizer can decompose phosphorus, prevent disease, promote growth, increase output, and improve product quality. The effect is stable.

The invention relates to a production method of a streptococcus faecium live bacteria preparation, belongs to the technical field of microorganisms, and in particular relates to a liquid anaerobic fermentation production method of the streptococcus faecium live bacteria preparation. The method comprises the following steps: by using streptococcus faecium as a fermenting culture, inoculating in an optimized liquid culture medium; cultivating for 16-20 hours at 40-42 DEG C; placing in a tank and measuring the number of live bacteria; absorbing the fermentation liquor by plant starch; and drying and crushing and packaging to prepare the streptococcus faecium live bacteria preparation with stable activity. The preparation provided by the invention has the advantages that high density fermentation of the streptococcus faecium live bacteria preparation can be completed by a common fermenting tank, and the preparation is simple to operate, stable in effect and low in energy consumption; devices such as a centrifugal machine and a freezer dryer are not used, so that the production cost is lowered and the production efficiency is improved, and the preparation is easy to popular and use.

The invention relates to the field of river management, in particular to a superior microbial acclimation method used for river water pollution management. The superior microbial acclimation method comprises the steps of microbial acclimation stoste preparation, enriched medium preparation and microbial acclimation. According to the method provided by the invention, a microbial acclimation stoste is prepared according to actual conditions of the water sample and the bottom mud of a river to be managed, then an enriched medium is prepared, and finally, through repeated and continuous microbial acclimations and cultivations, a microorganism mixed culture is obtained. In the microorganism mixed culture, enriched cultivating goal bacterium has absolute advantage. On one hand, the acclimation process is simple, the operation is easy, and the cost is low, and on the other hand, goal bacterium can quickly survive, adapt to environment and propagate, environmental requirements are low, acclimated goal bacterium is high in content and has an absolute advantage, and the effect of river water pollution management is good.

The invention relates to a method for high-density fermentation of bacillus subtilis. A mixed fermentation mode of combining liquid fermentation with solid fermentation is adopted, meanwhile, coconut shell powder is added to a solid fermentation culture medium; and the coconut shell powder contains 36.51% of lignin, 53.06% of cellulose and 29.27% of pentosan; and therefore, proliferation of bacillus subtilis can be promoted.

The invention discloses a preparation method for a synergistic bacterium type straw decomposing agent with high enzyme yield, and belongs to the technical field of straw decomposing agent preparation. The preparation method comprises the following steps: fermenting mixed strains which mainly comprise bacillus subtilis and trichoderma viride produced in a natural environment together; enabling the mixed strains which mainly comprise the bacillus subtilis and the trichoderma viride to perform natural symbiosis and competition to obtain mixed bacteria; selecting the strains which can produce cellulose through a screening culture medium; further improving the enzyme yield of the strains through irradiation improvement; providing attachments and nutrition sources by taking straws and wheat bran as mutant strains so as to improve the survival rate of the strains; freezing and drying to prepare the decomposing agent. According to the decomposing agent provided by the invention, the symbiotic synergistic effect among the strains is fully realized through natural screening; moreover, the content of effective bacteria is improved; the enzyme yield is increased; the activity is high; the decomposing agent has an extremely good straw decomposition promoting effect.

The invention relates to a method for fermenting soybean meal from sisal wastewater. To be specific, a waste by-product, namely, the sisal wastewater, of a sisal factory replaces externally-added water in the fermented soybean meal for soybean meal fermentation, special substances such as protease, organic acid and the like in the sisal wastewater are utilized, and the degradation rate of anti-nutritional factors in the soybean meal is greatly increased while the content of small peptides in the fermented soybean meal is increased. The method is simple in technology and low in cost, the problem of the sisal wastewater is solved for the sisal factory, the development of cyclic economy and environmental protection are promoted, the product quality of the fermented soybean meal is improved, and the content of the small peptides is greatly increased in particular.

The invention relates to a solid microbial agent for preventing diseases and increasing yield of potatoes and a preparation method, wherein the active ingredient of the solid microbial agent for preventing diseases and increasing yield of potatoes includes bacillus amyloliquefaciens PHODG36, and also contains a dispersing agent, a wetting agent and filler. The invention also discloses the method for preparing the microbial agent, which comprises the following steps: preparing the bacillus amyloliquefaciens PHODG36 fermentation liquor by fermentation, and fully and uniformly mixing the preparedfermentation liquor with other raw materials in a mechanical mixing manner, thereby obtaining the solid microbial agent. The invention also discloses a specific effect of the solid microbial agent onprevention and treatment of potato verticillium wilt and yield. By using the solid microbial agent disclosed by the invention, the occurrence of potato verticillium wilt can be greatly reduced, and the yield of potatoes can be remarkably increased.

The invention discloses a yoghurt starter culture medium and application thereof. The yoghurt starter culture medium comprises the following raw materials in percentage by weight: 3%-8% of fruit and vegetable juice and / or fruit and vegetable powder, 1%-2% of whey protein, 1%-2% of fish meat, 0.5%-1% of pork livers, 1.5%-2% of glucose power and / or high fructose syrup, 0.1%-0.2% of sodium citrate and the balance of purified water. All the raw materials of the yoghurt starter culture medium are food raw materials or food additives and are low in price and high in safety. According to the yoghurt starter culture medium, streptococcus thermophilus and lactobacillus bulgaricus are faster in growth and propagation and high in microbial content, and the microbial content can reach 109 CFU / mL for about 12 hours; in addition, because the yoghurt starter culture medium is rich in components, namely polypeptide, free amino acid and the like, a fermentation cultivation bacterium solution as a producing strain is directly inoculated to cow milk, and the formation of a yoghurt flavor substance is specially facilitated, so that a final yoghurt product is strong in flavor, short in yoghurt fermentation time and high in microbial content.

The invention discloses a liquid culture medium for industrialized production of stropharia rugoso-annulata. The liquid culture medium is prepared from 4-6 g / L soybean powder, 4-6 g / L corn powder, 4-6g / L bran powder, 25-35 g / L white granulated sugar, 0.5-1 g / L potassium dihydrogen phosphate, 0.5-1 g / L magnesium sulfate and 0.3-1 g / L peptone in proportion. The invention further discloses a preparation method of the liquid culture medium for the industrialized production of the stropharia rugoso-annulata. The preparation method comprises the following steps of grinding and sieving of the raw materials, stirring for dissolution, adjustment of the ph value, magnetic stirring and high-pressure sterilization. According to the liquid culture medium for the industrialized production of the stropharia rugoso-annulata, the sources of the raw materials of the culture medium are rich, the nutrients of the culture medium are rich, the growth period is short, and the nutrient requirements for the growth of the stropharia rugoso-annulata are met; the culture medium is applied to industrialized liquid strain production of the stropharia rugoso-annulata and has the advantages that the nutrients are rich, the fungus content of a liquid strain is high, fungus pellets are formed, the size is uniform, spawn running is quick after the fungus pellets are inoculated into a cultivation material, and the increase of the per unit yield of the stropharia rugoso-annulata and the improvement of the quality of the stropharia rugoso-annulata are facilitated.

The invention discloses an application of bacillus siamensis B11 in prevention and / or treatment of bamboo shoot blight. The separated bacillus siamensis B11 strain has a special effect on the bamboo shoot blight, is high in environmental suitability and good in affinity, is suitable for commercial production, meets the strategic requirement of sustainable development, and has a wide market prospect.

The invention discloses a preparation method of a solid decomposing inoculant, which comprises the following steps: 1, manufacturing a solid fermentation device; 2, preparing a seed solution; step 3, preparing fermentation liquor; and 4, carrying out solid fermentation to obtain the solid decomposition agent. The invention has the following beneficial effects: 1, bacteria and fungi are mixed in the product, spores are not easy to germinate after the fungi are fermented, the bacteria content is relatively stable after the bacteria are fermented, and the shelf life is longer than that of common products; 2, fermentation functional strains are directionally screened according to main components such as cellulose, protein, starch and lignin in a fermentation material in a test, and the product has the function of decomposing the cellulose, the protein, the starch and the lignin and is wide in application range; 3, a special fermentation formula for solid is researched through tests, and the bacterium content in unit product is increased, so that the use amount of the product is reduced, and the decomposition cost is reduced.