Method for cleanly and efficiently producing microorganism bactericide

A technology of microbial agent and production method, which is applied in the direction of microorganisms, microorganisms, biochemical equipment and methods, etc., can solve the problems of difficult product extraction and high energy consumption, and achieve the reduction of organic matter content, high utilization rate of nutrients, and high yield of products. high rate effect

Active Publication Date: 2013-04-03
HANGZHOU XIANGTIANGE BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to improve the difficulty in extracting the products of the existing liquid fermentation process and the problems of high energy consumption; the purpose of the present invention is also to reduce the content of organic matter in the fermentation wastewater, reduce the treatment cost of the fermentation wastewater, or directly reuse the product after obtaining it In the fermentation production of the product, the fermentation wastewater is zero-discharged; the purpose of the present invention is to increase the utilization rate of the culture substrate and the bacterial content per unit volume of the fermentation liquid by adding mineral carriers; the purpose of the present invention is to obtain the product not only Live microbial cells, as well as metabolites such as bacteriocins and enzymes, improve the use value of the product

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Fermentation of Bacillus subtilis by adding a composite carrier with a particle size of less than 10um in the fermentation medium

[0031] The specific steps are:

[0032] (1) Activate the freeze-dried bacteria stored at 4°C or the liquid bacteria stored at -80°C. Mark Bacillus subtilis ACCC10619 on the LB (peptone 10 g / L, yeast extract powder 5 g / L, sodium chloride 5 g / L) solid culture dish prepared in advance, and then place it in the Incubate for 18 hours in a biochemical incubator.

[0033] (2) Preparation of primary seed solution. Pick a typical uniform single colony from the activated culture dish and inoculate it into the prepared LB liquid sterile medium, then place it in a constant temperature shaking incubator, set the temperature at 37°C, and the rotation speed at 150rpm, and cultivate for 18 hours . Obtain first-class seed liquid.

[0034] (3) Preparation of secondary seed solution. Firstly, the air filter, air duct and fermenter of the fermentation sy...

Embodiment 2

[0042] Fermentation of Bacillus licheniformis by adding a composite carrier with a particle size of less than 50um in the fermentation medium

[0043] The specific steps are:

[0044] (1) Activate the freeze-dried bacteria stored at 4°C or the liquid bacteria stored at -80°C. Bacillus licheniformis CICC23584 was streaked on the LB solid culture dish prepared in advance, and then placed in a biochemical incubator set at 35°C for 24 hours.

[0045] (2) Preparation of primary seed solution. Pick a typical uniform single colony from the activated culture dish and inoculate it into the LB liquid sterile medium prepared in advance, then place it in a constant temperature shaking incubator, set the temperature at 32°C and rotate at 150rpm, and incubate for 22 hours . Obtain first-class seed liquid.

[0046] (3) Preparation of secondary seed solution. Firstly, the air filter, air duct and fermenter of the fermentation system for making the seed liquid are sterilized with steam, a...

Embodiment 3

[0055] Fermentation of Pseudomonas stutzeri by adding zeolite powder with particle size less than 50um in the fermentation medium

[0056] The specific steps are:

[0057] (1) Activate the freeze-dried bacteria stored at 4°C or the liquid bacteria stored at -80°C. Pseudomonas stutzeri CICC23621 was streaked on the LB solid culture dish prepared in advance, and then placed in a biochemical incubator set at 30°C for 24 hours.

[0058] (2) Preparation of primary seed solution. Pick a typical uniform single colony from the activated petri dish and inoculate it into the prepared LB liquid sterile medium, then place it in a constant temperature shaking incubator, set the temperature at 30°C, and the rotation speed at 150rpm, and incubate for 22 hours . Obtain first-class seed liquid.

[0059] (3) Preparation of secondary seed solution. Firstly, the air filter, air duct and fermenter of the fermentation system for making the seed liquid are sterilized with steam, and then the pr...

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PUM

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Abstract

The invention relates to a method for cleanly and efficiently producing a microorganism bactericide, and the method comprises the following steps: adding adsorptive carriers in a liquid yeast culture medium, such as one or more of active carbon, zeolite, medical stone, sepiolite, active soil, diatomite, montmorillonite, attapulgite or all other artificial porous adsorptive materials, carrying outhigh-temperature sterilization together with a culture medium, cooling at a proper temperature, inserting bacteria, fermenting according to proper bacteria breeding conditions, wherein viable bacteria and metabolic product are adsorbed on the carriers after the fermentation is completed; and quickly settling after standing, and collecting a settlement, and drying or drying after pelletizing the settlement. A supernatant fluid after the fermentation product is collected and can be used as recycled water after being subjected to sterilization, or be used for preparing a culture medium for recycling.

Description

technical field [0001] The invention relates to a clean and high-efficiency production method for microbial bacterial agents, belonging to the field of fermentation engineering. Background technique [0002] Microbial agents refer to live microorganisms and their metabolites obtained from one or more strains of microorganisms through a series of fermentation and post-processing processes. Its dosage forms are liquid, powder or granules. The main fermentation methods are solid fermentation and liquid fermentation. The main uses are: used as medicine, feed additive, crop insecticide, used for crop disease prevention and production increase, ecological environment protection and pollution prevention and control. Among them, environmental protection microbial agents are widely used in the improvement of aquaculture water, domestic waste treatment, harmless treatment of livestock and poultry manure, denitrification and phosphorus removal of industrial and domestic sewage, and C...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/00C12N11/14C12N11/00
Inventor 黄海智邱晓力储卫华黄丽丽陈瑞
Owner HANGZHOU XIANGTIANGE BIO TECH
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