Liquid culture medium for industrialized production of stropharia rugoso-annulata and preparation method thereof
A technology of liquid culture medium and oyster mushroom, applied in the directions of biochemical equipment and methods, botanical equipment and methods, microorganism-based methods, etc., can solve the production and application blanks of liquid strains, stay, and less research on liquid strains and other problems, to achieve the effect of uniform size, improving unit yield and quality, and high content of liquid bacteria
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Embodiment 1
[0019] A kind of industrial production liquid culture medium of Stropharia stropharia, is made up of the raw material of following ratio: soybean powder 6g / L, corn flour 6g / L, bran powder 6g / L, white granulated sugar 30g / L, dihydrogen phosphate Potassium 1g / L, magnesium sulfate 0.6g / L, peptone 0.3g / L.
[0020] A method for preparing a liquid culture medium for industrial production of stropharia, comprising the following steps:
[0021] S1: Grinding soybean flour, corn flour, bran flour, white granulated sugar, potassium dihydrogen phosphate, magnesium sulfate and peptone respectively, and then crushing and sieving with a 200-mesh sieve;
[0022] S2: Accurately weigh each raw material according to the proportion of the formula, put it into the bucket, stir and dissolve evenly;
[0023] S3: Dilute to the volume of the prepared medium, adjust the pH to 6.0;
[0024] S4: Use a dispenser to dispense the liquid culture medium with a constant volume into 1000ml Erlenmeyer flasks w...
Embodiment 2
[0027] A kind of industrial production liquid culture medium of Stropharia stropharia, is made up of the raw material of following ratio: soybean powder 5g / L, corn flour 5g / L, bran powder 5g / L, white granulated sugar 35g / L, dihydrogen phosphate Potassium 1g / L, magnesium sulfate 0.6g / L, peptone 0.6g / L.
[0028] A method for preparing a liquid culture medium for industrial production of Stropharia as above, comprising the following steps:
[0029] S1: Grinding soybean flour, corn flour, bran flour, white granulated sugar, potassium dihydrogen phosphate, magnesium sulfate and peptone respectively, and then crushing and sieving with a 200-mesh sieve;
[0030] S2: Accurately weigh each raw material according to the proportion of the formula, put it into the bucket, stir and dissolve evenly;
[0031] S3: Dilute to the volume of the prepared medium, adjust the pH to 6.1;
[0032] S4: Use a dispenser to dispense the liquid culture medium with a constant volume into 1000ml Erlenmeyer...
Embodiment 3
[0035] A kind of industrial production liquid culture medium of Stropharia stropharia, is made up of the raw material of following ratio: soybean powder 6g / L, corn flour 6g / L, bran powder 6g / L, white granulated sugar 30g / L, dihydrogen phosphate Potassium 0.6g / L, magnesium sulfate 0.6g / L, peptone 0.6g / L.
[0036] A method for preparing a liquid culture medium for industrial production of stropharia, comprising the following steps:
[0037] S1: Grinding soybean flour, corn flour, bran flour, white granulated sugar, potassium dihydrogen phosphate, magnesium sulfate and peptone respectively, and then crushing and sieving with a 200-mesh sieve;
[0038] S2: Accurately weigh each raw material according to the proportion of the formula, put it into the bucket, stir and dissolve evenly;
[0039] S3: Dilute to the volume of the prepared medium, and adjust the pH to 6.2;
[0040] S4: Use a dispenser to dispense the liquid culture medium with a constant volume into 1000ml Erlenmeyer fl...
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