Liquid culture medium for industrialized production of stropharia rugoso-annulata and preparation method thereof

A technology of liquid culture medium and oyster mushroom, applied in the directions of biochemical equipment and methods, botanical equipment and methods, microorganism-based methods, etc., can solve the production and application blanks of liquid strains, stay, and less research on liquid strains and other problems, to achieve the effect of uniform size, improving unit yield and quality, and high content of liquid bacteria

Inactive Publication Date: 2019-12-17
江苏东越生物技术发展股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] As a newcomer in the fungus world and a blue ocean in the industry, Stropharia stropharia urgently needs to carry out a lot of basic research work, especially the problem of strain production that needs to be solved for industrialization development. At present, most of the

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A kind of industrial production liquid culture medium of Stropharia stropharia, is made up of the raw material of following ratio: soybean powder 6g / L, corn flour 6g / L, bran powder 6g / L, white granulated sugar 30g / L, dihydrogen phosphate Potassium 1g / L, magnesium sulfate 0.6g / L, peptone 0.3g / L.

[0020] A method for preparing a liquid culture medium for industrial production of stropharia, comprising the following steps:

[0021] S1: Grinding soybean flour, corn flour, bran flour, white granulated sugar, potassium dihydrogen phosphate, magnesium sulfate and peptone respectively, and then crushing and sieving with a 200-mesh sieve;

[0022] S2: Accurately weigh each raw material according to the proportion of the formula, put it into the bucket, stir and dissolve evenly;

[0023] S3: Dilute to the volume of the prepared medium, adjust the pH to 6.0;

[0024] S4: Use a dispenser to dispense the liquid culture medium with a constant volume into 1000ml Erlenmeyer flasks w...

Embodiment 2

[0027] A kind of industrial production liquid culture medium of Stropharia stropharia, is made up of the raw material of following ratio: soybean powder 5g / L, corn flour 5g / L, bran powder 5g / L, white granulated sugar 35g / L, dihydrogen phosphate Potassium 1g / L, magnesium sulfate 0.6g / L, peptone 0.6g / L.

[0028] A method for preparing a liquid culture medium for industrial production of Stropharia as above, comprising the following steps:

[0029] S1: Grinding soybean flour, corn flour, bran flour, white granulated sugar, potassium dihydrogen phosphate, magnesium sulfate and peptone respectively, and then crushing and sieving with a 200-mesh sieve;

[0030] S2: Accurately weigh each raw material according to the proportion of the formula, put it into the bucket, stir and dissolve evenly;

[0031] S3: Dilute to the volume of the prepared medium, adjust the pH to 6.1;

[0032] S4: Use a dispenser to dispense the liquid culture medium with a constant volume into 1000ml Erlenmeyer...

Embodiment 3

[0035] A kind of industrial production liquid culture medium of Stropharia stropharia, is made up of the raw material of following ratio: soybean powder 6g / L, corn flour 6g / L, bran powder 6g / L, white granulated sugar 30g / L, dihydrogen phosphate Potassium 0.6g / L, magnesium sulfate 0.6g / L, peptone 0.6g / L.

[0036] A method for preparing a liquid culture medium for industrial production of stropharia, comprising the following steps:

[0037] S1: Grinding soybean flour, corn flour, bran flour, white granulated sugar, potassium dihydrogen phosphate, magnesium sulfate and peptone respectively, and then crushing and sieving with a 200-mesh sieve;

[0038] S2: Accurately weigh each raw material according to the proportion of the formula, put it into the bucket, stir and dissolve evenly;

[0039] S3: Dilute to the volume of the prepared medium, and adjust the pH to 6.2;

[0040] S4: Use a dispenser to dispense the liquid culture medium with a constant volume into 1000ml Erlenmeyer fl...

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PUM

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Abstract

The invention discloses a liquid culture medium for industrialized production of stropharia rugoso-annulata. The liquid culture medium is prepared from 4-6 g/L soybean powder, 4-6 g/L corn powder, 4-6g/L bran powder, 25-35 g/L white granulated sugar, 0.5-1 g/L potassium dihydrogen phosphate, 0.5-1 g/L magnesium sulfate and 0.3-1 g/L peptone in proportion. The invention further discloses a preparation method of the liquid culture medium for the industrialized production of the stropharia rugoso-annulata. The preparation method comprises the following steps of grinding and sieving of the raw materials, stirring for dissolution, adjustment of the ph value, magnetic stirring and high-pressure sterilization. According to the liquid culture medium for the industrialized production of the stropharia rugoso-annulata, the sources of the raw materials of the culture medium are rich, the nutrients of the culture medium are rich, the growth period is short, and the nutrient requirements for the growth of the stropharia rugoso-annulata are met; the culture medium is applied to industrialized liquid strain production of the stropharia rugoso-annulata and has the advantages that the nutrients are rich, the fungus content of a liquid strain is high, fungus pellets are formed, the size is uniform, spawn running is quick after the fungus pellets are inoculated into a cultivation material, and the increase of the per unit yield of the stropharia rugoso-annulata and the improvement of the quality of the stropharia rugoso-annulata are facilitated.

Description

technical field [0001] The invention belongs to the technical field of edible fungus cultivation, and in particular relates to a liquid medium for industrial production of Stropharia stropharia and a preparation method thereof. Background technique [0002] The large stropharia is also known as the wrinkled stropharia, stropharia stropharia, wine red stropharia, brown stropharia, stropharia stropharia, kidney-nourishing mushroom, colorful cloud mushroom, etc., and is commonly known as "red matsutake". The color is bright, the mushroom shape is beautiful, the cover is soft and smooth, the handle is crisp, the taste is fragrant, and the nutrition is rich, which is very popular among consumers. It is one of the characteristic mushrooms recommended by the International Food and Agriculture Organization for cultivation in developing countries, and it is also one of the top ten traded mushrooms in the international edible fungus market. Stropharia is an expert in biodegrading crop...

Claims

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Application Information

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IPC IPC(8): A01G18/20C12N1/14C12R1/645
CPCA01G18/20C12N1/14
Inventor 刘记水
Owner 江苏东越生物技术发展股份有限公司
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