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Preparation method for synergistic bacterium type straw decomposing agent with high enzyme yield

A technology of straw decomposing agent and bacterial type, which is applied in the field of preparation of high-yield enzyme-amount synergistic bacterial type straw decomposing agent, can solve the problems of low bacterial content, small enzyme activity, and poor decomposing effect, and achieve high bacterial content , the content of enzyme activity is increased, and the effect of promoting the decomposing effect

Inactive Publication Date: 2017-04-26
孙祎
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention: most of the present straw decomposing agents are prepared by mixing various bacterial agents in a certain proportion, and the product has a low bacterial content and too small enzyme activity, and the enzyme production of the bacterial strains cannot be considered The symbiosis and antagonism between the ability and strains lead to the disadvantages of poor decomposing effect. A method is provided that uses dry rice straw extract and wood chips as fermentation substrates, inoculates Trichoderma viride and ferments in pumpkins to produce bacteria, and transfers mycelia to screen A method for producing compound strains of cellulase, mutagenizing with cobalt 60, and finally blending and fermenting with rice straw and wheat bran to prepare a decomposing agent

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0017] Take a fresh pumpkin, dig a round hole with a diameter of 10 cm at the center point of the melon pedicle, hollow out the inside of the pumpkin from the round hole to obtain a hollow pumpkin, then collect the dried straw and put it in a jet mill to crush it into 100-mesh hay fragments. Then, according to the mass ratio of 1:10, after mixing the hay fragments and the biogas liquid, put them into a fermenter for sealing and fermentation for 3 days, after the fermentation ends, filter and separate to remove the filter residue to obtain the hay extract; Mix and stir the straw extract and sawdust to form a slurry, add glucose with a mass of 10% of the slurry to the slurry, continue to stir evenly, and then put it into the above-mentioned hollow pumpkin. Add Trichoderma viride strains with a mass of 3% of the slurry to the slurry, seal the round hole on the pumpkin with plastic wrap, then put the pumpkin in the greenhouse and ferment for 15 days at 25°C; The surface of the slu...

example 2

[0020]Take a fresh pumpkin, dig a round hole with a diameter of 10 cm at the center point of the melon pedicle, hollow out the inside of the pumpkin from the round hole to obtain a hollow pumpkin, then collect the dried straw and put it in a jet mill to crush it into 100-mesh hay fragments. Then, according to the mass ratio of 1:10, after mixing the hay fragments and the biogas liquid, put them into a fermenter for sealing and fermentation for 4 days, after the fermentation, filter and separate to remove the filter residue to obtain the hay extract; Mix and stir the straw extract and sawdust to form a slurry, add glucose with a mass of 10% of the slurry to the slurry, continue to stir evenly, and then put it into the above-mentioned hollow pumpkin. Add Trichoderma viride strains with a mass of 3% of the slurry to the slurry, seal the round hole on the pumpkin with plastic wrap, then put the pumpkin in the greenhouse and ferment for 18 days at 30°C; The surface of the slurry is...

example 3

[0023] Take a fresh pumpkin, dig a round hole with a diameter of 10 cm at the center point of the melon pedicle, hollow out the inside of the pumpkin from the round hole to obtain a hollow pumpkin, then collect the dried straw and put it in a jet mill to crush it into 100-mesh hay fragments. Then, according to the mass ratio of 1:10, after mixing the hay fragments and the biogas liquid, put them into a fermenter and seal them for fermentation for 5 days. Mix and stir the straw extract and sawdust to form a slurry, add glucose with a mass of 10% of the slurry to the slurry, continue to stir evenly, and then put it into the above-mentioned hollow pumpkin. Add Trichoderma viride strains with a mass of 3% of the slurry to the slurry, seal the round hole on the pumpkin with plastic wrap, then put the pumpkin in the greenhouse and ferment for 20 days at 35°C; The surface of the slurry is covered with hyphae, open the plastic wrap, and use an inoculation loop to evenly select 10 loop...

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Abstract

The invention discloses a preparation method for a synergistic bacterium type straw decomposing agent with high enzyme yield, and belongs to the technical field of straw decomposing agent preparation. The preparation method comprises the following steps: fermenting mixed strains which mainly comprise bacillus subtilis and trichoderma viride produced in a natural environment together; enabling the mixed strains which mainly comprise the bacillus subtilis and the trichoderma viride to perform natural symbiosis and competition to obtain mixed bacteria; selecting the strains which can produce cellulose through a screening culture medium; further improving the enzyme yield of the strains through irradiation improvement; providing attachments and nutrition sources by taking straws and wheat bran as mutant strains so as to improve the survival rate of the strains; freezing and drying to prepare the decomposing agent. According to the decomposing agent provided by the invention, the symbiotic synergistic effect among the strains is fully realized through natural screening; moreover, the content of effective bacteria is improved; the enzyme yield is increased; the activity is high; the decomposing agent has an extremely good straw decomposition promoting effect.

Description

technical field [0001] The invention relates to a preparation method of a high-yield enzyme-amount synergistic bacteria-type straw decomposing agent, which belongs to the technical field of straw decomposing agent preparation. Background technique [0002] In addition to carbon substances, crop straw also contains elements such as nitrogen, phosphorus, potassium, calcium, and magnesium, as well as nutrients or beneficial components such as cellulose, hemicellulose, lignin, protein, amino acids, and organic matter. Therefore, crop straw is a very important renewable resource in agricultural production. The processing methods of crop straws in our country include incineration and direct return to the field. The incineration not only wastes resources, but also produces a lot of smoke and pollutes the environment, affecting the health of humans and animals and traffic safety. Although the direct return to the field can make use of crop The straw itself and the microorganisms in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/01C12N13/00C12N1/20C12N1/14C12R1/885C12R1/125
CPCC12N15/01C12N1/14C12N1/20C12N13/00
Inventor 孙祎王树兰丁春美
Owner 孙祎
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