Streptococcus faecium live bacteria preparation and preparation method thereof

A technology of Streptococcus faecalis and live bacteria preparations, which is applied in the field of preparation of probiotic feed additives through anaerobic fermentation of Streptococcus faecalis in liquid culture medium, which can solve the problems of insufficient density of viable bacteria in fermentation products, non-reusable raw materials, complex processes, etc. , to achieve the effects of inhibiting bacterial contamination, shortening the fermentation cycle, and complicating the operation

Inactive Publication Date: 2013-09-11
GUANGZHOU GLAM BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The previous fermentation method of Streptococcus faecalis mainly has the following problems and deficiencies: (1) The anaerobic culture tank is used or the anaerobic environment is maintained by continuously feeding carbon dioxide or nitrogen. The raw materials cannot be reused, and the operation is more complicated and the cost is higher. High; (2) The post-processing of liquid fermentation products requires high-speed refrigerated centrifuges for centrifugation and low-temperature freeze-drying, the process is complicated, the cost is high, and the yield is low; (3) The viable bacteria density of fermentation products is not high enough

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1 The technical process for preparing the stabilized Streptococcus faecalis live bacteria preparation comprises the following steps:

[0027] (1) Strain activation:

[0028] The present invention uses Streptococcus faecalis as the fermentation strain, which is purchased from the Guangdong Provincial Microbial Strain Preservation Management Center, preservation number: GIM1.130, and the live Streptococcus faecalis strain preserved at -80°C is stored in MRS medium (peptone 10.0 g , beef extract 10.0 g, yeast extract 5.0 g, diammonium hydrogen citrate 2.0 g, glucose 20.0 g, Tween 80 1.0 mL, sodium acetate 5.0 g, dipotassium hydrogen phosphate 2.0 g, magnesium sulfate 0.58 g, manganese sulfate 0.25 g , agar powder 15.0 g, distilled water 1 000 mL, pH 6.2~6.6, high-temperature and high-pressure sterilization) to draw a line on the solid slant, incubate at 37°C for about 16 hours, and the bacteria are generally activated 1 to 3 times;

[0029] (2) Prep...

Embodiment 2

[0037] Embodiment 2 is different from above-mentioned embodiment 1:

[0038] (1) Medium formula: corn flour 60, wheat flour 25, bean cake powder 15, glucose 10, yeast extract powder 1.5, peptone 3.0, the raw materials should be calculated in grams, and the corresponding parts in mL of distilled water should be 800~1000. 100°C, 30min high temperature and high pressure sterilization, when the temperature of the fermenter is lowered to 35~45°C, add 1.0g / 1000mL of inorganic salt base material, the composition of inorganic salt: MgSO 4· 7H 2 O 0.04%, MnSO 4 ·H 2 O 0.04%, in order to avoid the formation of precipitation, stir evenly during the addition process, then add liquid paraffin to a thickness of about 1cm;

[0039] (2) Temperature-controlled fermentation: The inoculum amount of Streptococcus faecalis liquid seeds was 3%, stirred evenly, the initial pH was 6.6, the pH was controlled at 6.0-6.8, the fermentation temperature was controlled at 37-40°C, and the fermentation ...

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Abstract

The invention relates to a production method of a streptococcus faecium live bacteria preparation, belongs to the technical field of microorganisms, and in particular relates to a liquid anaerobic fermentation production method of the streptococcus faecium live bacteria preparation. The method comprises the following steps: by using streptococcus faecium as a fermenting culture, inoculating in an optimized liquid culture medium; cultivating for 16-20 hours at 40-42 DEG C; placing in a tank and measuring the number of live bacteria; absorbing the fermentation liquor by plant starch; and drying and crushing and packaging to prepare the streptococcus faecium live bacteria preparation with stable activity. The preparation provided by the invention has the advantages that high density fermentation of the streptococcus faecium live bacteria preparation can be completed by a common fermenting tank, and the preparation is simple to operate, stable in effect and low in energy consumption; devices such as a centrifugal machine and a freezer dryer are not used, so that the production cost is lowered and the production efficiency is improved, and the preparation is easy to popular and use.

Description

[0001] technical field [0002] The invention relates to the field of microbial fermentation engineering, in particular to a method for preparing a probiotic feed additive through anaerobic fermentation of streptococcus faecalis in a liquid medium. Background technique [0003] Streptococcus faecalis ( Enterococcus faecalis ) is also known as Enterococcus faecalis, its shape is streptococcus or spherical, the diameter of the bacteria is 0.3 μm to 0.7 μm, and there are no spores; the colony formed on the broth agar plate after dilution is round and milky white, and the surface is convex and wet , shiny, with neat edges, most arranged in pairs or short chains, usually immobile. Colonies on rich medium are large and smooth, 1-2 mm in diameter. It has low nutritional requirements and can grow on ordinary nutrient agar. It can grow in pH 9.6 or 6.5% NaCl broth medium at 10°C or 45°C, and can withstand high temperature at 65°C for 30 minutes. It can use arginine as energy, fe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/00A23K1/16C12N1/20C12R1/46
Inventor 李帅伟李本涛
Owner GUANGZHOU GLAM BIOTECH
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