Streptococcus faecium live bacteria preparation and preparation method thereof
A technology of Streptococcus faecalis and live bacteria preparations, which is applied in the field of preparation of probiotic feed additives through anaerobic fermentation of Streptococcus faecalis in liquid culture medium, which can solve the problems of insufficient density of viable bacteria in fermentation products, non-reusable raw materials, complex processes, etc. , to achieve the effects of inhibiting bacterial contamination, shortening the fermentation cycle, and complicating the operation
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Embodiment 1
[0026] Embodiment 1 The technical process for preparing the stabilized Streptococcus faecalis live bacteria preparation comprises the following steps:
[0027] (1) Strain activation:
[0028] The present invention uses Streptococcus faecalis as the fermentation strain, which is purchased from the Guangdong Provincial Microbial Strain Preservation Management Center, preservation number: GIM1.130, and the live Streptococcus faecalis strain preserved at -80°C is stored in MRS medium (peptone 10.0 g , beef extract 10.0 g, yeast extract 5.0 g, diammonium hydrogen citrate 2.0 g, glucose 20.0 g, Tween 80 1.0 mL, sodium acetate 5.0 g, dipotassium hydrogen phosphate 2.0 g, magnesium sulfate 0.58 g, manganese sulfate 0.25 g , agar powder 15.0 g, distilled water 1 000 mL, pH 6.2~6.6, high-temperature and high-pressure sterilization) to draw a line on the solid slant, incubate at 37°C for about 16 hours, and the bacteria are generally activated 1 to 3 times;
[0029] (2) Prep...
Embodiment 2
[0037] Embodiment 2 is different from above-mentioned embodiment 1:
[0038] (1) Medium formula: corn flour 60, wheat flour 25, bean cake powder 15, glucose 10, yeast extract powder 1.5, peptone 3.0, the raw materials should be calculated in grams, and the corresponding parts in mL of distilled water should be 800~1000. 100°C, 30min high temperature and high pressure sterilization, when the temperature of the fermenter is lowered to 35~45°C, add 1.0g / 1000mL of inorganic salt base material, the composition of inorganic salt: MgSO 4· 7H 2 O 0.04%, MnSO 4 ·H 2 O 0.04%, in order to avoid the formation of precipitation, stir evenly during the addition process, then add liquid paraffin to a thickness of about 1cm;
[0039] (2) Temperature-controlled fermentation: The inoculum amount of Streptococcus faecalis liquid seeds was 3%, stirred evenly, the initial pH was 6.6, the pH was controlled at 6.0-6.8, the fermentation temperature was controlled at 37-40°C, and the fermentation ...
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