36 results about "Streptococcus fecalis" patented technology

The invention provides a method for detecting fluorescent quantitative PCR of 12 pathogenic bacteria in real time at the same time, including the steps: collecting specific pathogenic gene or toxin gene of the target pathogen and using it as a target gene to design primers and probes so as to make the reaction conditions consistent; extracting a genome template of a sample to be detected; adding the template respectively into tubules equipped with different specific upstream and downstream primers and probes, and then adding the corresponding fluorescent quantitative PCR reagents; under the same cycle of fluorescent quantitative PCR, the corresponding primers and probes are used to detect the samples simultaneously, quickly and quantitatively in their respective reaction tubes. Easier, Quick and efficient, Twelve common pathogenic bacteria (Escherichia coli O157: H7, Listeria monocytogenes, Salmonella, Vibrio parahaemolyticus, Streptococcus betae, Yersinia enterocolitica, Streptococcusfaecalis, Shigella, Proteus mirabilis, Vibrio fluvialis, Campylobacter jejuni, Staphylococcus aureus) can be detected simultaneously in drinking water and food economically.

The invention relates to a composite biological living bacteria preparation and a preparation method thereof. The total number of effective viable bacteria is not less than 108cfu/ml. The method includes: selecting optimal probiotics, carrying out supporting medium activation, performing enlarged culture and mixing, conducting culture by means of a mixed bacteria medium and a fermentation tank, carrying out filtration, concentration and proportioning so as to form the composite living bacteria preparation. The preparation contains the following bacteria with the total number of effective viable bacteria of: 22-25% of photosynthetic bacteria, 21-24% of yeast, 15-20% of bacillus subtilis, 15-20% of Pediococcus acidilactici, 12-16% of Streptococcus faecalis, and 12-16% of Lactobacillus acidophilus. The composite biological living bacteria preparation provided by the invention can be applied to agricultural cultivation, livestock and poultry breeding, aquaculture, feed adding, municipal sewage and domestic garbage treatment and other fields.

The invention discloses an aga lysing biological preparation and an application thereof. The preparation is prepared through mixing Bacillus subtilis, Bacillus licheniformis, Bacillus laterosporus, Streptococcus faecalis, photosynthetic bacteria, Sphingomonas sp., vitamin C, vitamin E and lactose. The above product has good stability and high efficiency, and consumes an extremely low amount of oxygen and rapidly degrades microcystis and other pollutants in culture water in the application process in order to effectively improve the environment of the water. The preparation is nontoxic and harmless to environment, people and animals, has no residuals, can substantially improve and purify the environment and the culture body and effectively avoid bursting of cyanobacterial bloom, and also can improve the immunity of aquatic product animals and reduce the incidence of diseases to make the ecologic system of the whole culture water tend to balance and go well.

The invention discloses a chromogenic medium and a quick test slip of streptococcus faecium. The chromogenic medium of the streptococcus faecium comprises peptone, yeast powder, sodium chloride, lactose, maltose, TTC (Triphenyltetrazolium Chloride), a bacteriostatic agent and an indicator. The quick test slip is a stable microbial culture device comprising a base plate, a water absorbing gel layer, a culture medium layer and a covering film; the chromogenic medium is absorbed on the culture medium layer. The chromogenic medium of the streptococcus faecium is accurate in testing results, obvious in display of positive results, high in specificity, convenient to observe and capable of performing qualitative detection and quantitative analysis to the streptococcus faecium at the same time. According to the quick test slip of the streptococcus faecium, the conventional multi-step detection and identification process is simplified to be done in one step; and in addition, the test slip is small in size and convenient to carry, can sample on site at any time, is simple and convenient to operate, and provides scientific basis and guidance for the water quality safety.

The invention discloses a Streptococcus faecalis powdering LAMP rapid detection kit, and a rapid detection method based on the kit. The kit comprises a freeze-dried isothermal amplification agent, a re-dissolving solution, a positive contrast dry powder, a negative contrast substance, a colorimetric solution, a lysate and a sealing solution, and the freeze-dried isothermal amplification agent contains dNTPs, Bst DNA polymerase, an amplimer and a freeze-drying protection agent. The primer in the kit corresponds to eight segments of a Streptococcus faecalis specific gene sequence, the colorimetric solution is added after a reaction, and a negative and positive colorimetric difference is obvious. The kit has the advantages of strong specificity, high sensitivity, easiness in operation and simplicity in result judgment, and is suitable for the quality control and the safe and rapid detection of foods, beverages, water and other raw and auxiliary materials. The kit is convenient for normal temperature transportation, is simple and fast to use without special devices, reduces the cost, widens the application range of products, is suitable for onsite rapid detection and base remote region related detection, and has wide application prospect.

The invention relates to a Pseudobagrus fulvidraco feed. The invention is characterized in that the Pseudobagrus fulvidraco feed comprises the following raw materials in parts by weight: 0.5-0.8 part of silicate bacterium, 1-3 parts of Streptococcus faecalis, 0.6-0.8 part of Trichoderma, 2-5 parts of Rhodopseudomonas palustris, 0.3-0.5 part of microzyme, 50-60 parts of fishbone dust, 65-70 parts of vinegar residue powder, 50-55 parts of waste liquor precipitate, 40-60 parts of rice bran, 12-14 parts of brown sugar, 10-14 parts of table salt, 80-85 parts of pig manure, 45-55 parts of pork abattoir waste, 60-65 parts of aquatic, 12-16 parts of calcium bicarbonate, 14-17 parts of urea, 1-3 parts of ferric citrate, 0.6-0.8 part of vitamin B1, 0.2-0.6 part of vitamin B12, 20-30 parts of pepper and the like. A comparison shows that the composite microbial fertilizer can promote quick growth of stichopus japonicus, increase the EPA content, reduce the fish diseases and the amount of fish dying of illness, and increase the beneficial microbes in the water body, thereby enhancing the final quality and grade.

The invention discloses a mixed strain, strain vector and method capable of continuously degrading total ammonia nitrogen in black and odorous water in a river. The mixed strain consists of the following microorganisms: bacillus subtilis, bacillus licheniformis, photosynthetic bacteria, EM bacteria, streptococcus faecalis, lactobacillus, lactobacillus plantarum, lactobacillus acidophilus, nitrobacteria and denitrifying bacteria (all strains are commercially available); and moreover, all microorganisms contained in the mixed strain are domesticated into plant-sourced microorganisms, and the mixed strain is mixed with short-chain fatty acid. By adopting the synergism of various means, the total ammonia nitrogen contained in the black and odor water in the river is effectively treated.

The invention discloses an organic waste fermentation method which comprises the following steps: stirring organic waste and compound bacteria, and stacking into a fermentation pile; covering the fermentation pile with a breathable film; introducing a gas containing oxygen into the fermentation heap; wherein the compound bacteria comprise bacillus subtilis, bacillus subtilis natto, bacillus brevis, lactobacillus acidophilus, lactobacillus plantarum, lactobacillus delbrueckii, streptococcus faecalis, streptococcus lactis, streptococcus thermophilus, candida utilis, pichia pastoris, nitrifying bacteria, methane acidifying bacteria, sulfur reducing bacteria and photosynthetic bacteria. The organic waste fermentation method provided by the invention can quickly ferment, heat and evaporate water, and quickly decompose the generated harmful smell, thereby achieving the goal of innocent treatment of organic wastes.

The invention relates to the technical field of microbial preparation, and discloses an environment-friendly biological agent for secondary fermentation of an organic fertilizer. The environment-friendly biological agent comprises 400-500 parts of pig manure, 70-110 parts of sweet potato seedlings, 60-100 parts of a nitrogen phosphorus potassium fertilizer, 70-100 parts of soybean meal, 20-40 parts of diatomite, 80-120 parts of stone straw, 10-30 parts of saccharides, 10-20 parts of waste fungus spores, 50-100 parts of turf, 50-80 parts of rice hull powder, 30-50 parts of mushroom residues, 15-25 parts of streptococcus faecalis, 4-7 parts of saccharomycetes, 3-5 parts of cellulase, 5-15 parts of cassava residues, 5-15 parts of sawdust, 6-14 parts of distillers' grains, 3-8 parts of wheat bran, 2-4 parts of tobacco powder and 2-4 parts of furfural residues. The environment-friendly biological agent for secondary fermentation of the organic fertilizer has the advantages of being comprehensive in nutrient and capable of achieving the effects of improving soil, improving soil fertility, increasing yield, enhancing quality and the like; and the selenium content of the soil can be remarkably increased by applying a large amount of the environment-friendly biological agent for the organic fertilizer, and therefore, the absorption performance of the soil is improved.

The invention belongs to the technical field of ecological breeding, and discloses a preparation method of traditional Chinese medicine probiotic fermentation liquor for fish feed. The preparation method comprises the following steps: decocting nine traditional Chinese medicines, namely andrographis paniculata, radix isatidis, cyrtomium fortunei, radix astragali seu hedysari, codonopsis pilosula, red dates, pericarpium citri reticulatae, hawthorn and liquorice, and brown sugar in water, diluting a formed decoction, conducting heating to 80-100 DEG C, and adding starch for gelatinization reaction to obtain a suspension fermentation culture solution; and adding composite bacterial powder consisting of bacillus subtilis, saccharomycetes, streptococcus faecalis and rhodospirillum into the suspension fermentation culture solution, conducting stirring, conducting mixing and dispersing uniformly, then adding a nitrogen source and inorganic salt, conducting mixing uniformly, and carrying out suspension fermentation culture under the temperature condition of 10-38 DEG C and the air condition until white to beige fermentation bacterial plaques grow on the surface of the suspension fermentation culture solution, so as to obtain the traditional Chinese medicine probiotic fermentation liquor. The preparation process is simple, and the obtained fermentation liquor is used for feed preparation and can remarkably reduce the feed coefficient.

The invention discloses a dual fluorescent quantitative PCR (Polymerase Chain Reaction) detection method and kit for two pathogenic bacteria in pre-packaged drinking water. The kit comprises primers and probes (SEQ ID NO: 1-6) for detecting the pseudomonas aeruginosa and the streptococcus faecalis. The kit is good in specificity, the detection method is rapid and simple, the accuracy is high, the sensitivity is good, and a new method is provided for detecting the two pathogenic bacteria of the pseudomonas aeruginosa and the streptococcus faecalis in the pre-packaged drinking water. The dual fluorescent quantitative PCR method provided by the invention can accurately and rapidly realize detection of pseudomonas aeruginosa and streptococcus faecalis in drinking water, can complete detection within one day, has the advantages of rapidness, specificity, sensitivity, high flux, good stability, reagent cost saving and the like, and is suitable for two pathogenic bacteria indexes of pseudomonas aeruginosa and streptococcus faecalis in drinking water standards.

The invention discloses a preparation and use method of a biochemical agent for biological treatment, the biochemical agent comprises a plant nutrient, a microbial carrier and fish feed, the plant nutrient comprises cytokinin, gibberellin, furacilin, gentamicin, forchlorfenuron and the like; the microbial carrier is composed of rhodopseudomonas palustris, photosynthetic bacteria, lactic acid bacteria, streptococcus faecalis, clostridium butyricum and the like. The feed can eliminate harmful germs in a water body and inhibit fester and suppuration at the injured part of the body surface of the fish, the quality of the water body is improved while the fish body is treated, high-nutrient substances are provided for the fish by feeding fish feed, and the resistance of the fish body is improved; adjustment from the fish body and the environment is beneficial for accelerating recovery of the fish body, and the pretreatment method for the water body can be suitable for optimizing water quality of a local water area and improving the environment; when the first nutrient substance is prepared, the concentrated mineral substance can also be applied to industries such as food, beverage, medical treatment, health care, beauty, make-up, wine brewing and pet supplies.

The invention provides a probiotic honey grapefruit tea and a preparation method thereof. In the technical scheme, lactobacillus casei and streptococcus faecium are taken as probiotic components and are blended with a conventional beverage, so that an intestinal tract probiotic effect is achieved. On such basis, the invention researches an approach for inhibiting growth of two probiotics, in orderto prevent the lactobacillus casei and streptococcus faecium from propagating in the beverage and influencing the flavor of the beverage. An experiment proves that an antagonism effect on propagationefficiency can be achieved when two probiotics are co-cultured with pyrococcus furiosus and methanosarcina barkeri; on such basis, an intergrowth environment and a nutritional condition of four microorganisms are researched; a treating method according to the invention can guarantee the biomass accumulation in early stage and can achieve the balance of flora scale of four microorganisms after mixed cultivation; the blending of the beverage and the mixed bacteria acquired according to the method is capable of keeping bacteria propagation basically stagnated under storage and transportation environments, so that the flavor of the beverage is prevented from being influenced.