37 results about "Streptococcus fecalis" patented technology

Disclosed herein are brown algae-derived fucoidan and in particular a method for degrading fucoidan to a low-molecular weight so that fucoidan as sparingly-degradable polysaccharide (M.W. 3,000,000 to 5,000,000) can be more efficiently extracted from brown algae and it can be absorbed in the human body. The method comprises preparing a fucoidan extract containing as much fucoidan as possible from brown algae selected from Hizikia fusiforme, Undaria pinnatifida, Laminaria saccharina, Ecklonia cava, Cladosiphon Okamuranus and Nemacystus decipiens, and low-molecularizating the fucoidan extract using Vibrio halioticoli (Accession No. KCTC 10998BP) and a probiotic mixture containing Streptococcus faecalis, Bacillus mesentericus, Clostridium butyricum, and glycosyltransferase thereof. According to the method, fucoidan can be efficiently degraded to a low molecular weight using microorganisms so that it can be absorbed in the human body. The fucoidan obtained realizes practical utilization of beneficial efficiencies thereof in a variety of fields, in particular, health foods, meals for patients (e.g., cancer patients), medicine, cosmetics, agriculture, fishing and stockbreeding.

The invention discloses a compound microbial fish fertilizer special for silver carp and a preparation method thereof. The compound microbial fish fertilizer special for silver carp comprises the following components in parts by weight: 0.5-0.8 part of silicate bacteria, 1-3 parts of streptococcus faecalis, 0.6-0.8 part of trichoderma sp, 2-5 parts of rhodopseudomonas palustris, 0.3-0.5 part of saccharomycetes, 50-60 parts of fishbone dust, 65-70 parts of vinegar residue powder, 50-55 parts of effluent precipitate, 40-60 parts of rice bran, 12-14 parts of brown sugar, 10-14 parts of table salt, 80-85 parts of pig manure, 45-55 parts of waste of a pig meat slaughter house, 60-65 parts of aquatic plants, 12-16 parts of calcium bicarbonate, 14-17 parts of urea, 1-3 parts of ferric citrate, 0.6-0.8 part of vitamin B1, 0.2-0.6 part of Vitamin B12, 1-3 parts of rheum officinale, 2-6 parts of Astragalus membranaceus and 4-7 parts of medicated leaven. The fertilizer disclosed by the invention can promote silver carp to grow quickly, the EPA content of fish is increased, the fish diseases are reduced, and the number of dead fish is reduced, and meanwhile, beneficial microorganisms in water are increased. Finally, both the quality and the weight of silver carp are improved.

The invention discloses a quadruple fluorescent PCR primer set for detecting four pathogenic bacteria in drinking water. The quadruple fluorescent PCR primer set comprises primers for coli groups, streptococcus faecalis, pseudomonas aeruginosa and clostridium perfringens, and is specifically shown as Seq. ID No. 1 to Seq. ID No. 8. The invention belongs to the technical field of gene engineering detection, mutual interference cannot be caused for each primer and each probe, amplification can be carried out on specific objective sequences only, a fluorescence signal is stimulated, non-objectivesequences are free of amplification or fluorescence signal, accurate detection for the four pathogenic bacteria in drinking water can be realized, and the quadruple fluorescent PCR primer set has theadvantages of high specificity, small standard error, short detection time, reagent cost saving and the like and is applicable for detecting the indexes of the pathogenic bacteria in the standard ofdrinking water.

The invention relates to a method for symbiotic culture of streptococcus faecalis and saccharomycetes. The technical scheme is that the optimum formulation of a culture medium is 2.4 g% peptone, 1.2-2.4 g% glucose, and 0.5-1 g% yeast powder, and the initial PH value is 7.0; the cultivation method comprises the following steps: the inoculum sizes of saccharomycetes seed solution and streptococcus faecalis seed solution are 2.5% and 1.0% respectively, the streptococcus faecalis seed solution is firstly inoculated in the culture medium to be cultured to the early exponential phase (about 4 hours), then the saccharomycetes seed solution is added, and at the temperature of 30 DEG C, the oscillating rotational speed is 180 rpm, the cultivation time is 20 hours, and next streptococcus faecalis and saccharomycetes are subjected to enlarge cultivation in a 5L fermentation tank, wherein the cultivation condition is that the throughput is 2.4 Lpm, the stirring rate is 100 rpm, the tank temperature is 30 DEG C, the broth content is 60%, and the cultivation time is 24 hours. The method is low in cost and stable in action effect, improves the number of compound bacteria, and enhances the action effect of a streptococcus faecalis and saccharomycetes complex microbial inoculant.

The present invention provides a triple vital bacteria composition, including: powder of Bifidobacterium longum, powder of Lactobacillus acidophilus, and powder of Streptococcus faecalis. The present invention further provides a method for preparing the above triple vital bacteria composition, as well as the protection agents, the fermenting culture medium and the seed culture medium used therein.

The invention discloses a biological preparation for balancing water quality for aquaculture. The biological preparation comprises the components of: sodium humate accounting for 49 percent, sodium thiosulfate accounting for 35 percent, biological flocculants accounting for 10 percent, bacillus subtilis accounting for 3 percent, streptococcus faecium accounting for 3 percent, wherein the bacillus subtilis is 3,000,000,000 / g and the streptococcus faecium is 3,000,000,000 / g. According to the biological preparation, the nutrition of a water body is balanced, the water quality is improved, the blue-green algae outburst is controlled, the growth of beneficial algae is activated, dissolved oxygen of the water body is increased, the content of beneficial microorganisms in the water body is increased, the occurrence of chronic nitrate and nitrous acid poison incidents of fishes can be prevented, the immune globulin and the antibody level of the fishes are improved, and the herd immunity of the fishes is improved; and by adding the streptococcus faecium, the true nutritive value of feed is improved, the concentration of ammoniacal nitrogen in excrements also can be reduced, and the pollution to the water quality is reduced.