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Charcoal-based microbial compound bacterial powder, bacterial agent as well as preparation method and application of charcoal-based microbial compound bacterial agent

A technology of composite bacterial powder and composite bacterial agent, which is applied in the field of microorganisms, can solve problems such as high operating costs, large investment, and unsatisfactory effects, and achieve the effects of improving processing performance and reducing pollution

Pending Publication Date: 2020-01-24
SHANGHAI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although these methods can remove nitrogen, phosphorus, organic matter and heavy metals in some wastewater, they have disadvantages such as large investment, high operating cost, and complicated treatment process, and the effect is not ideal.

Method used

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  • Charcoal-based microbial compound bacterial powder, bacterial agent as well as preparation method and application of charcoal-based microbial compound bacterial agent
  • Charcoal-based microbial compound bacterial powder, bacterial agent as well as preparation method and application of charcoal-based microbial compound bacterial agent
  • Charcoal-based microbial compound bacterial powder, bacterial agent as well as preparation method and application of charcoal-based microbial compound bacterial agent

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preparation example Construction

[0032] In the present invention, the solid carrier includes biochar and zeolite, and the mass ratio of biochar to zeolite is preferably 20-30:20-30, more preferably 25:25. The present invention has no special limitation on the source of the biochar, which can be prepared by conventional commercially available products or conventional methods. In the specific embodiment of the present invention, the preparation method of the biochar preferably includes: thermally cracking the waste left over from the processing of bamboo products at 450-550°C under anaerobic conditions for 1-5 hours, and passing through 80-100 meshes after crushing Sieve, the under-sieve is biochar. The present invention has no special limitation on the source of the zeolite, and it can be prepared by using commercially available products or conventional preparation methods. In the specific embodiment of the present invention, the preparation method of the zeolite preferably includes: , crushed, passed through...

Embodiment 1

[0045] Preparation of carbon-based microbial composite bacterial powder:

[0046] 1) Various microorganisms used for sewage treatment are sequentially subjected to seed liquid culture and liquid fermentation culture to obtain a composite microbial fermentation liquid;

[0047] 2) Mixing and adsorbing the composite microbial fermentation liquid with biochar and zeolite carrier according to the mass ratio of 1:25:25 to prepare the carbon-based carrier microbial composite bacterial liquid;

[0048] 3) drying at 30°C, crushing, and passing through a 40-mesh sieve is carbon-based microbial composite bacterial powder, with a water content below 15%.

[0049] Among them, the microorganisms are Bacillus and streptococcus faecalis, the mass ratio is 3:1, and the Bacillus is Bacillus subtilis (CGMCC1. Bacillus (CGMCC1.10291), the preservation number of Streptococcus faecalis is CGMCC1.15321.

[0050] The microbial seed liquid culture steps for sewage treatment are as follows:

[0051...

Embodiment 2

[0059] Preparation of carbon-based microbial composite bacterial powder:

[0060] 1) Various microorganisms used for sewage treatment are sequentially subjected to seed liquid culture and liquid fermentation culture to obtain a composite microbial fermentation liquid;

[0061] 2) Mixing and adsorbing the composite microbial fermentation liquid with biochar and zeolite carrier according to the mass ratio of 1:20:20 to prepare the carbon-based carrier microbial composite bacterial liquid;

[0062] 3) drying at 30°C, crushing, and passing through a 40-mesh sieve is carbon-based microbial composite bacterial powder, with a water content below 15%.

[0063] All the other conditions are with embodiment 1.

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Abstract

The invention provides charcoal-based microbial compound bacterial powder, bacterial agent as well as a preparation method and application of the charcoal-based microbial compound bacterial agent andbelongs to the technical field of microbes. Microbes are adsorbed on a solid carrier to obtain the charcoal-based microbial compound bacterial agent; the solid carrier comprises charcoal and zeolite;microbes comprise bacillus and streptococcus faecalis; the mass ratio of the solid carrier and microbes is (40-60):1. The high porosity and high specific surface area of the charcoal are used and matched with high-adsorption material zeolite, the treatment performance of single microbial agent for livestock and poultry breeding sewage is improved, the pH of water is kept stable, nitrogen, phosphorus, organic pollutants and harmful heavy metal residues in wastewater are effectively reduced, and the pollution degree of surrounding water environments by livestock and poultry breeding sewage is relieved.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a carbon-based microbial composite bacterial powder, a bacterial agent, a preparation method and an application thereof. Background technique [0002] With the rapid development of large-scale, intensive and modernized livestock and poultry breeding industry in China, the discharge of livestock and poultry breeding sewage has increased sharply, and the problem of breeding pollution has become more and more prominent. According to incomplete statistics, in 2011, the output of livestock and poultry manure and urine in my country was about 2.7 billion tons, which was about twice the total industrial solid waste discharge in that year; livestock and poultry manure and urine contained a large amount of organic pollutants, COD alone has reached 120 million tons, far exceeding the sum of COD in industrial and domestic waste, and has become one of the three major source...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C02F3/34
CPCC02F3/34C12N11/14
Inventor 秦秦薛永宋科孙丽娟孙雅菲
Owner SHANGHAI ACAD OF AGRI SCI
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