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Lactobacillus acidophilus/enterococcus faecalis culture medium, preparations and technique

一种嗜酸乳杆菌、粪链球菌的技术，应用在链球菌/乳球菌、乳杆菌、双歧杆菌等方向，能够解决稳定性的效果不十分令人满意等问题，达到活菌收得率提高、提高稳定性、提高存活率的效果

Active Publication Date: 2013-09-25
SHANGHAI SINE PHARMA LAB
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the use of the above protective agents can improve the stability of Bifidobacterium, Lactobacillus acidophilus and Streptococcus faecalis to a certain extent, the effect of further improving the stability is not very satisfactory
[0004] In addition, there are currently no literature reports to improve the seed liquid culture medium and fermentation medium of Bifidobacterium, Lactobacillus acidophilus and Streptococcus faecalis to improve the stability of Bifidobacterium, Lactobacillus acidophilus and Streptococcus faecalis

Method used

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  • Lactobacillus acidophilus/enterococcus faecalis culture medium, preparations and technique
  • Lactobacillus acidophilus/enterococcus faecalis culture medium, preparations and technique
  • Lactobacillus acidophilus/enterococcus faecalis culture medium, preparations and technique

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0114] Embodiment 1: Preparation of the seed culture medium inoculated with Bifidobacterium longum

[0115] Use 500ml of pure water to dissolve the glucose (purchased from Hebei Shengxue Glucose Co., Ltd.), yeast powder (Germany MERCK KGAA.), ammonium sulfate (Jiangsu Yonghua Fine Chemicals Co., Ltd., AR grade), Potassium dihydrogen phosphate (Shantou Jinsha Chemical Factory Co., Ltd., AR grade), dipotassium hydrogen phosphate (Shantou Jinsha Chemical Factory Co., Ltd., AR grade) and allolactose (Italy INALCO S.P.A.) to obtain a solution. And use 1500ml of pure water to beat the skimmed milk powder (FONTERRALTD., New Zealand) of the quantity shown in the following table 1 with a homogenizer (Shanghai Specimen Model Factory, DS-1) to obtain a suspension.

[0116] Above-mentioned solution and suspension are put into the seed bottle after colloid mill (Wenzhou Lucheng Emulsification Machinery Company, JM-L80) grinds evenly, then add to 4000g with drinking water and prepare the ...

Embodiment 2

[0121] Embodiment 2: Preparation of the seed culture medium inoculated with Lactobacillus acidophilus and Streptococcus faecalis

[0122] Use 500ml of pure water to dissolve the glucose (Hebei Shengxue Glucose Co., Ltd.), yeast powder (Germany MERCK KGAA.), lactose (Shanghai Shenmei Pharmaceutical Development Technology Co., Ltd.), tomato juice (Shanghai Pudong New Area Jinyang Street Xinxin Non-staple Food Business Department), to get the solution. And use 1500ml of pure water to beat the tryptone (German MERCK KGAA.) and peptone (Nippon Pharmaceutical Co., Ltd.) in the quantity shown in the following table 2 with a homogenizer (Shanghai Specimen Model Factory, DS-1) to obtain a suspension.

[0123] Put the above solution and suspension into the seed bottle after being uniformly ground by a colloid mill, then add drinking water to 4000g to prepare the seed medium of Bifidobacterium longum, and then add a little dilute sodium hydroxide solution to adjust the pH of the medium...

Embodiment 3

[0128] Embodiment 3: make each bifidobacterium longum fermentation medium

[0129] Pour the components shown in Table 3 below except calcium carbonate into a stainless steel bucket, then pour 30 kg of drinking water into the stainless steel bucket, and stir with a stainless steel rod at the same time to obtain a suspension of the culture medium. Pour the resulting suspension into a colloid mill and grind evenly. Pour the uniformly ground suspension into a fermenter equipped with a stirrer, and replenish to 400kg with water. Turn on the fermenter mixer, adjust the pH value of the medium with NaOH, then add the calcium carbonate shown in Table 3 below, and add water to 400kg at the same time to obtain the fermentation medium. The fermentation culture was sterilized based on 121°C for 10-15 minutes.

[0130] table 3

[0131] 400kg preparation amount

[0132] components Prescription 1 Prescription 2 Prescription 3 Prescription 4 Prescription 5 skimme...

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Abstract

The present invention provides a triple vital bacteria composition, including: powder of Bifidobacterium longum, powder of Lactobacillus acidophilus, and powder of Streptococcus faecalis. The present invention further provides a method for preparing the above triple vital bacteria composition, as well as the protection agents, the fermenting culture medium and the seed culture medium used therein.

Description

technical field [0001] The invention relates to a triple live bacteria preparation and a preparation method thereof, more specifically to a triple live bacteria preparation comprising Bifidobacterium longum, Lactobacillus acidophilus and Streptococcus faecalis and a preparation method thereof. Background technique [0002] Bifidobacterium, Lactobacillus acidophilus and Streptococcus faecalis are important members of the normal flora found in humans and animals. They adhere concentratedly to the lower intestinal mucosa, exert beneficial effects on the body, and can inhibit the invasion of foreign pathogenic bacteria. Their metabolites provide Essential vitamins for the host, participate in and assist the digestion and absorption of nutrients. The above-mentioned bacteria can stimulate the immune function of the body. Reduce absorption of gut-derived toxicity. Bifidobacteria in the body gradually decrease due to digestive system diseases or heavy use of antibiotics and with ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61K35/744A61K35/745A61K35/747
CPCA61K35/745C12N1/20A23Y2240/33A23V2002/00A61K35/747A23L1/3014A23Y2300/55A61K35/744A23Y2220/03C12N1/04A23L33/135Y10S435/853Y10S435/854Y10S435/885A61P1/00A61P1/10A61P1/12A23V2200/32A23V2200/3204A23V2400/113A23V2400/225A23V2400/533
Inventor 陈彬华王雪松张亮
Owner SHANGHAI SINE PHARMA LAB
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