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Alga lysing biological preparation and application thereof

A biological preparation and algae-dissolving technology, which is applied in the direction of biocide, application, biocide, etc., can solve the problems of achieving good results, the difficulty of microorganisms to Microcystis, etc., and achieve a reasonable ratio, good degradation and improvement , the effect of high concentration of live bacteria

Active Publication Date: 2016-02-10
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Once the cyanobacteria bloom breaks out in the aquaculture water body, it is difficult for microorganisms to control Microcystis to achieve good results within an effective time

Method used

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  • Alga lysing biological preparation and application thereof
  • Alga lysing biological preparation and application thereof
  • Alga lysing biological preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1-4

[0037] A kind of algae-dissolving biological preparation is formulated by weight parts from the following raw materials:

[0038]

Example 1 Example 2 Example 3 Example 4 Bacillus subtilis 1 2 1 1 Bacillus licheniformis 3 3 1 1 Bacillus paraspora 2.5 2.8 2.5 2 Streptococcus faecalis 2 1.5 1 1 Photosynthetic bacteria 2.5 2 2 2 Sphingomonas 0.8 0.5 1 1 Vitamin C 0.5 1 1 0.5 Vitamin E 0.5 0.5 0.6 1 lactose 0.2 0.2 0.3 0.3

[0039] The bacteria powders of Bacillus licheniformis and Bacillus lateralis used in the embodiment of the present invention were purchased from Hubei Lvtiandi Biotechnology Co., Ltd.; the powders of Sphingomonas and Bacillus subtilis were purchased from Shanghai Loeb Biotechnology Co., Ltd., each The bacteria content is 30 billion / g. Streptococcus faecalis and photosynthetic bacteria powder were purchased from Hubei Tianchen Biotechnology Co., Ltd., and the...

Embodiment 5

[0043] Algicidal effect of algicidal biological preparations with different formulations:

[0044] 500ml of Microcystis aeruginosa 905 algae solution at an algae concentration of 1*10 7 When cell / ml, algae-dissolving biological agent was added at a ratio of 10 mg / L, monitored every 12 hours, and three parallels were made for each treatment group. Microcystis aeruginosa was cultured with light (12:12 hours light / dark cycle) using BG11 algae medium, and the removal effect of alginolytic biological agents on Microcystis was calculated. The absorbance (OD680) at the wavelength of 680nm was used as the index of Microcystis population density. An increase in the data represents good growth of the algae, a decrease in the data represents the death of the algae, and the data are expressed as mean ± standard error (SE).

[0045] According to the formula: removal rate = (m-n) / m*100 (m: OD of algae solution without adding any substance680 The value of; n = algae solution OD after addin...

Embodiment 6

[0051] A kind of application of algae-dissolving biological agent, its application process is:

[0052] Take by weighing powdery microbial bacterial agent, vitamin and lactose according to the ratio of Example 1, take an appropriate amount of the mixed powder algae-dissolving composition material according to the ratio of 0.01g / L, drop into Microcystis aeruginosa solution, its stock solution OD 680 = 0.58, the absorbance value of algae cells in the water body was measured every 12 hours, and the total treatment and monitoring was 72 hours.

[0053] The specific preparation and application process of Examples 2, 3, and 4 are as in Example 1.

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Abstract

The invention discloses an aga lysing biological preparation and an application thereof. The preparation is prepared through mixing Bacillus subtilis, Bacillus licheniformis, Bacillus laterosporus, Streptococcus faecalis, photosynthetic bacteria, Sphingomonas sp., vitamin C, vitamin E and lactose. The above product has good stability and high efficiency, and consumes an extremely low amount of oxygen and rapidly degrades microcystis and other pollutants in culture water in the application process in order to effectively improve the environment of the water. The preparation is nontoxic and harmless to environment, people and animals, has no residuals, can substantially improve and purify the environment and the culture body and effectively avoid bursting of cyanobacterial bloom, and also can improve the immunity of aquatic product animals and reduce the incidence of diseases to make the ecologic system of the whole culture water tend to balance and go well.

Description

technical field [0001] The invention relates to the application field of removing blue-green algae in eutrophic water bodies, more specifically relates to an algae-dissolving biological preparation, and also relates to the application of the preparation in removing blue-green algae. Background technique [0002] In recent years, with the intensification of eutrophication in global water bodies, the frequency and magnitude of harmful algal blooms around the world are increasing day by day. Algal blooms have become a global problem that seriously affects the quality of the water environment and the ecological security of water bodies. It not only greatly endangers human health and other biological safety, but also causes huge losses to the global economy. Therefore, how to quickly control and eliminate algae bloom pollution has become a hot issue in the field of water environment. The water environment is closely related to human life, and it also seriously affects the develo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/00A01P13/00A01P1/00C02F3/34
Inventor 张学振陈元元魏晋吴康
Owner HUAZHONG AGRI UNIV
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